Effect of Oxygen on Several Enzymes Involved in the Aerobic and Anaerobic Utilization of Glucose in<i>Escherichia coli</i>

Thomas, A.D.; Doelle, Horst W.; Westwood, A.; Gordon, Geoffrey L. R.

doi:10.1128/jb.112.3.1099-1105.1972

Cited by 62 publications

(18 citation statements)

References 22 publications

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“…This would cause a metabolic shift toward a flux state described by MS2. These predictions appear to be consistent with reported experimental data since a-ketoglutarate dehydrogenase is down-regulated at both a genetic and enzymatic level during oxygen limitation (Amarasingham and Davis, 1965;Thomas et al, 1972). In addition, the modes predict that a shift from MS1 to MS2 would result in acetate production.…”

Section: Observed Regulation Patterns Are Consistent With Identified supporting

confidence: 88%

Fundamental Escherichia coli biochemical pathways for biomass and energy production: Identification of reactions

Carlson

Srienc

2003

Biotech & Bioengineering

105

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Cells grow by oxidizing nutrients using a complex network of biochemical reactions. During this process new biological material is produced along with energy used for maintaining cellular organization. Because the metabolic network is highly branched, these tasks can be accomplished using a wide variety of unique reaction sequences. However, evolutionary pressures under carbon-limited growth conditions likely select organisms that utilize highly efficient pathways. Using elementary-mode analysis, we demonstrate that the metabolism of the bacterium Escherichia coli contains four unique pathways that most efficiently convert glucose and oxygen into new cells and maintenance energy under any level of oxygen limitation. Observed regulatory patterns and experimental findings suggest growing cells use these highly efficient pathways. It is predicted that five knockout mutations generate a strain that supports growth using only the most efficient reaction sequence. The analysis approach should be generally useful for predicting metabolic capabilities and efficient network designs based on only genomic information.

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Section: Observed Regulation Patterns Are Consistent With Identified supporting

confidence: 88%

Fundamental Escherichia coli biochemical pathways for biomass and energy production: Identification of reactions

Carlson

Srienc

2003

Biotech & Bioengineering

105

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“…7K and L). The phosphofructokinase activity, which catalyses the irreversible, rate-limiting step of glycolysis, is highly regulated (Thomas et al, 1972;Doelle, 1975;Kotlarz et al, 1975), and the assay conditions may not reflect critical requirements for activity of this enzyme, such as the concentration of regulatory metabolites or relative oxygen tension.…”

Section: Adaptation Of Carbon Metabolism Of M Tuberculosis To Stressmentioning

confidence: 99%

Carbon flux rerouting during Mycobacterium tuberculosis growth arrest

Shi¹,

Sohaskey

Pfeiffer

et al. 2010

Molecular Microbiology

124

119

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SummaryA hallmark of the Mycobacterium tuberculosis life cycle is the pathogen's ability to switch between replicative and non-replicative states in response to host immunity. Transcriptional profiling by qPCR of~50 M. tuberculosis genes involved in central and lipid metabolism revealed a re-routing of carbon flow associated with bacterial growth arrest during mouse lung infection. Carbon rerouting was marked by a switch from metabolic pathways generating energy and biosynthetic precursors in growing bacilli to pathways for storage compound synthesis during growth arrest. Results of flux balance analysis using an in silico metabolic network were consistent with the transcript abundance data obtained in vivo. Similar transcriptional changes were seen in vitro when M. tuberculosis cultures were treated with bacteriostatic stressors under different nutritional conditions. Thus, altered expression of key metabolic genes reflects growth rate changes rather than changes in substrate availability. A model describing carbon flux rerouting was formulated that (i) provides a coherent interpretation of the adaptation of M. tuberculosis metabolism to immunity-induced stress and (ii) identifies features common to mycobacterial dormancy and stress responses of other organisms.

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“…A pH-inducible promoter and the production P-galactosidase were used as a model system. The pH-inducible promoter system has several distinct advantages: (1) The induction procedure involves straightforward changes in the medium pH and thus does not require any new equipment since monitoring and control of pH is simple and standard practice in most fermentation processes; (2) bulk chemicals are employed to change the reactor pH and therefore the induction protocol can be accomplished very inexpensively; and (3) the pH-inducible promoter is very strong, which allows high levels of recombinant protein production.…”

Section: Resultsmentioning

confidence: 99%

Strategies in High‐Level Expression of Recombinant Protein in Escherichia coli

San¹,

Bennett

Aristidou³

et al. 1994

Annals of the New York Academy of Sciences

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The accumulation of acetate is one of the most commonly encountered problems in attaining high levels of recombinant protein production using E. coli. Two different approaches are examined to reduce the rate of acetate formation. The effects of reduced acetate accumulation on recombinant protein production were also investigated. In the first approach, E. coli mutant strains deficient in enzymes involved in the acetate synthesis pathways were isolated and characterized. The level of specific production of beta-galactosidase by the mutant strain is three times higher than its parent strain. In another approach, metabolic engineering techniques were employed to fine-tune the central metabolic pathways to reduce the amount of acetate formation. The resulting strain, which carries the acetolactase synthase gene from B. subtilis, is successful in maintaining a very low level of acetate accumulation. The ALS-containing strain is also capable of producing higher levels of recombinant protein than its parent strain.

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Effect of Oxygen on Several Enzymes Involved in the Aerobic and Anaerobic Utilization of Glucose inEscherichia coli

Cited by 62 publications

References 22 publications

Fundamental Escherichia coli biochemical pathways for biomass and energy production: Identification of reactions

Fundamental Escherichia coli biochemical pathways for biomass and energy production: Identification of reactions

Carbon flux rerouting during Mycobacterium tuberculosis growth arrest

Strategies in High‐Level Expression of Recombinant Protein in Escherichia coli

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