Effect of oxygenated polyethylene glycol decorated hemoglobin on microvascular diameter and functional capillary density in the transgenic mouse model of sickle cell anemia

Abstract

Malemide polyethylene glycol-conjugated Hb (MP4OX, Sangart Inc.), a high-affinity low-concentration acellular hemoglobin (P50 = 5 mmHg, 4.3 g/dl) solution, has been shown to optimize microvascular perfusion and target oxygen delivery to anoxic tissue. Microvascular perfusion during an acute hypoxic challenge in a transgenic anemic sickle cell disease mouse model was studied with MP4OX and saline. Arterioles were dilated in both groups. Functional capillary density (FCD) was maintained at a higher level with MP4OX. In conclusion, MP4OX treatment reduced the hypoxia-mediated decline in FCD, an effect in part due to higher arterial pressure resulting in increased microvascular perfusion pressures.

Artificial Oxygen Carriers—Past, Present, and Future—a Review of the Most Innovative and Clinically Relevant Concepts

“…After unloading CO, the compound is oxygenated in the lung and thereby transforms into MP4OX. The effects of MP4OX have been further investigated in animal models of sickle cell anemia (Tsai et al, 2015). Relevant clinical trials with Hemospan/ MP4OX and MP4CO are listed in Supplemental Table 3.…”

The artificial oxygen carrier erythrocruorin—characteristics and potential significance in medicine

“…. As a consequence, clinical trials have been halted in 2011 [30] and none of the listed polyHbs have been approved for general use by the U.S. Food and Drug Administration (FDA) or European Medicines Agency [15,[31][32][33][34][35][36].…”