Effect of oxytetracycline on performance and microbial community of an anoxic–aerobic sequencing batch reactor treating mariculture wastewater

Abstract

The performance and microbial community of an anoxic-aerobic sequencing batch reactor (SBR) treating mariculture wastewater were evaluated at different oxytetracycline (OTC) concentrations. The COD and nitrogen removal efficiencies decreased with the increase in OTC concentration from 0 to 12 mg L À1 .No apparent NO 3 À -N accumulation was found during the whole operational period, whereas NO 2 À -N accumulation occurred at 12 mg L À1 OTC. The specific oxygen uptake rate (SOUR), specific ammonium oxidation rate (SAOR), specific nitrite oxidation rate (SNOR) and specific nitrate reduction rate (SNRR) decreased with increasing influent OTC concentration. The protein (PN) and polysaccharide (PS) contents in the loosely-bound extracellular polymeric substances (LB-EPS) and tightly bound EPS (TB-EPS) increased with the increase in OTC concentration from 0 to 12 mg L À1 , respectively. The appearance of OTC in the influent could obviously affect the functional groups of the PN and PS in the LB-EPS and TB-EPS. X-ray photoelectron spectroscopy (XPS) illustrated that amino, carboxyl and hydroxyl groups in the EPS might be involved in the interaction between the EPS and OTC. The denaturing gradient gel electrophoresis (DGGE) profile indicated that some variations were found in the microbial community of the activated sludge with increasing OTC concentration. Some microorganisms were depleted or weakened with the increase in OTC concentration, whereas others became the predominant microorganisms due to their ability to adapt to the OTC toxicity.

Microbial community distribution and dominant bacterial species analysis in the bio-electrochemical system treating low concentration cefuroxime

“…The presence of Pharmaceutical and Personal Care Products (PPCPs) may induce the occurrence of new dominant microbial species [10]. Wang et al noted that a concentration change in oxytetracycline had effects on the performance of a microbial community of anoxicaerobic SBR treating mariculture wastewater [11]. Matos et al concluded that at a concentration of 50 lgÁL À1 , tetracycline influenced biofilm bacterial composition [12].…”

Ecological impact of the antibiotic ciprofloxacin on microbial community of aerobic activated sludge

“…For instance, erythromycin caused adverse effects on the structure and chemistry of activated sludge when its presence at 10 mg/L (Louvet et al, 2010;Pala-Ozkok & Orhon, 2013). Recently, Wang et al (2015) observed an inhibition on COD and nitrogen removal in an anoxic-aerobic reactor under the presence of antibiotic oxytetracycline of 10 mg/L. Kang et al (2018) observed a change in the microbial community of aerobic granular and suspended activated sludge in the presence of sulfamethoxazole at 2 µg/L.…”

Effect of Amoxicillin on Nitrogen Oxidation Bacteria Present in Activated Sludge: Respirometry Investigation

“…When the AMX concentration was greater than 50 mg L −1 , the reduction was 74.0%, which clearly states that AMX inhibited the use of oxygen by NOB from this range. Some antibiotics such as ampicillin, norfloxacin, sulfadiazine, and oxytetracycline have a negative effect on the OUR of activated sludge and biofilms [20,[36][37][38][39].…”