Effect of oxytetracycline on the biosafety, gut microbial diversity, immune gene expression and withdrawal period in Pacific whiteleg shrimp, Penaeus vannamei

Avunje, Satheesha; Patil, Prasanna Kumar; Ezaz, Wasim; Praveena, Ezhil; Ray, Arabinda; Baskaran, Viswanathan; Alavandi, S.V.; Puthiyedathu, Sandeep Kizhakkekarammal; Vijayan, Koyadan Kizhakedath

doi:10.1016/j.aquaculture.2021.736957

Cited by 9 publications

(8 citation statements)

References 42 publications

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“…In recent years, growing evidence illustrated that gut microflora play an important role in regulating metabolism and immunity of an animal (Avunje et al, 2021;Wu et al, 2021). In this study, it was found that the gut microflora of large-scale loach was significantly altered by dietary BVC supplementation, which could be another rationale for the observed improvement in survival and growth.…”

Section: Discussionmentioning

confidence: 59%

Impacts of dietary supplementation of bamboo vinegar and charcoal powder on growth performance, intestinal morphology, and gut microflora of large-scale loach Paramisgurnus dabryanus

Kil²,

et al. 2023

J. Ocean. Limnol.

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Although accumulating data demonstrate that dietary supplementation of bamboo vinegar and charcoal powder (BVC) can significantly strengthen the immunity and boost the growth of domestic animals, its application potency still awaits verification in commercial fish species such as the large-scale loach Paramisgurnus dabryanus. The impacts of 90-day dietary supplementation of 1% and 2% BVC on survival and growth performance of the loach, and on the intestinal morphological characteristics and gut microflora were analyzed. Our data show that the large-scale loach supplied with BVC at the experimental doses had significant higher survival rates and better growth performance (indicated by greater weight gain (1.13-1.14 times), higher specific growth rate (1.04 times), and lower feed conversion ratio (0.88-0.89 times)) compared to that of the control (P<0.05). Histological examination revealed significant longer villus (3.22-5.54 times), deeper crypt (1.77-1.87 times), and thicker muscle (1.59-3.17 times) in the intestines of large-scale loach fed with BVC (P<0.05). Furthermore, we found that the gut microflora consisted of significantly fewer proportions of potential pathogenic bacterial species (Aeromonas veronii and Escherichia coli), but significantly greater proportions of beneficial microbes (Lactococus raffinolactis and Faecalibacterium prausnitzii). Therefore, dietary intake of BVC can promote intestinal tract development and optimize gut microflora, by which the survival and growth of large-scale loach may be improved.

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Section: Discussionmentioning

confidence: 59%

Impacts of dietary supplementation of bamboo vinegar and charcoal powder on growth performance, intestinal morphology, and gut microflora of large-scale loach Paramisgurnus dabryanus

Kil²,

et al. 2023

J. Ocean. Limnol.

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“…Dose and regimen optimization, clinical outcomes, pharmacokinetics and pharmacodynamics, withdrawal period and antibiotic degradation have been extensively studied 94 . Safety, tissue distribution, depletion and withdrawal period of OTC have been reported extensively in Oreochromis spp., 95,96 Gambusia holbrooki , 97 Oncorhynchus mykiss , 98 Oreochromis niloticus , 99–101 Trachinotus blochii , 102 Pangasianodon hypophthalmus , 103 Labeo rohita , 104 Dicentrarchus labrax , 105 Sparus aurata 105,106 and P. vannamei etc 107 . OTC is known to rapidly degrade in aquaculture environments through photo and thermo‐degradation 108 .…”

Section: Discussionmentioning

confidence: 99%

“…94 Safety, tissue distribution, depletion and withdrawal period of OTC have been reported extensively in Oreochromis spp., 95,96 Gambusia holbrooki, 97 Oncorhynchus mykiss, 98 Oreochromis niloticus, [99][100][101] Trachinotus blochii, 102 Pangasianodon hypophthalmus, 103 Labeo rohita, 104 Dicentrarchus labrax, 105 Sparus aurata 105,106 and P. vannamei etc. 107 OTC is known to rapidly degrade in aquaculture environments through photo and thermo-degradation. 108 Further, in an outdoor environment in the mid-summer day, the half-life was reported to be 21-25 min in aquaculture waters.…”

Section: Chemicals Biologicals and Vmps Used In Indian Aquaculturementioning

confidence: 99%

Usage pattern of chemicals, biologicals and veterinary medicinal products in Indian aquaculture

Patil

Mishra

Pradhan

et al. 2022

Reviews in Aquaculture

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The use of chemicals, biologicals and veterinary medicinal products (VMPs) helps in healthy and sustainable fish production. Information on the use of these products is essential for assessing farming practices, potential human health and environmental risks. A questionnaire‐based nationwide survey covering aquaculture farms (n = 2936) producing carps, tilapia, pangasius and rainbow trout in freshwater and shrimp in brackishwater estimated the use of 52 different types of inputs which included disinfectants (597 g t−1), probiotics (2.28 kg t−1), environmental modifiers (22.82 kg t−1), nutritional supplements (1.96 kg t−1), natural anti‐infective agents (293 g t−1), herbicide and piscicides (844 g t−1), antibiotics (2 mg PCU−1), antifungal (4 mg PCU−1), and antiparasitic (14 mg PCU−1) agents. The bulk of these inputs was used for soil and water quality improvement and had low environmental and human safety concerns. The multivariate analysis revealed significant variation in the frequency and quantity of compounds use among farm groups. Redundancy analysis revealed a significant association between the number of products used and stocking density. The survey also showed a considerable influence of education and farming experience on the usage pattern of aquaculture inputs. Results of the study indicated greater reliance of farmers on the use of disinfectants for biosecurity, nutritional supplements for enhanced growth and environmental modifiers for maintaining soil and water quality in culture systems. Though there was no use of restricted antibiotics and antiparasitic agents, the development and implementation of standard regulatory guidelines are essential for safe and effective use of inputs for sustainable aquaculture.

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“…The withdrawal time is the time required to eliminate drug residues in edible tissues to a safe level for consumers [61]. MRLs for CFT have not been established in European legislation or elsewhere.…”

Section: Cefotaxime Depletion In White Leg Shrimpmentioning

confidence: 99%

“…In the current research, after 3 days of CFT medication at the dose of 25 mg/kg, the CFT WTs were lower than those of other groups of antibiotics. For example, the WT of oxytetracycline in white leg shrimp after oral administration at the dose of 4.5 g/kg feed for 14 consecutive days was 96 h at 29 • C (corresponding to 116 degree-days), for the MRL of 100 µg/kg set by the EU, Canada and India [61]; in another study of Wang et al [14], the WTs in shrimp muscle of chloramphenicol, sulfamethoxazole and oxytetracycline at the dose of 2 g/kg feed after twice-a-day oral administration for 3 consecutive days were 139.7 h (139.7 degree-days), 30.6 h (30.6 degree-days) and 90.3 h (90.3 degree-days) at 24 • C, respectively.…”

Section: Cefotaxime Depletion In White Leg Shrimpmentioning

confidence: 99%

Pharmacokinetics and Withdrawal Times of Cefotaxime in White Leg Shrimp (Litopenaeus vannamei) after Oral Administration

Huynh,

Scippo,

Devreese

et al. 2024

Fishes

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A high-performance liquid chromatography method coupled to tandem mass spectrometry was validated in order to study the pharmacokinetics of cefotaxime in shrimp hepatopancreases and plasma, as well as its withdrawal time related to a maximum residue limit (MRL) in shrimp muscle. Pharmacokinetics parameters were investigated through oral medication at a single dose of 25 mg/kg shrimp body weight and subsequent hepatopancreas and plasma cefotaxime concentration measurements at 0.5, 1, 2, 4, 8, 12 and 24 h after shrimp were fed with medication. The maximum concentration of cefotaxime was observed after one hour in the hepatopancreas (Cmax, 19.45 ± 2.10 mg/kg) and 4 h in plasma (0.184 ± 0.061 mg/L). Based on a minimum inhibitory concentration (MIC) of cefotaxime of 4.13 mg/L against Vibrio parahaemolyticus (known to cause acute hepatopancreatic necrosis disease (AHPND) in white leg shrimp), it was observed that the time during which the hepatopancreas cefotaxime concentration was above the MIC was 23 h. An every 24 h cefotaxime treatment could thus be effective in fighting against this bacterium in shrimp. The withdrawal time of cefotaxime was determined after shrimp were fed with medicated feed once a day and twice a day for three consecutive days. Shrimp muscle was collected on day 1 and day 3 during medication and 1, 3, 7, 14 and 21 days after medication was stopped. Considering an MRL of 50 μg/kg, the withdrawal times were 8.5 degree-days (corresponding to 6.9 h at 29.5 °C) after shrimp were fed with medicated feed once a day for 3 days and 95.5 degree-days (77.7 h at 29.5 °C) after shrimp were fed with medicated feed twice a day for 3 days. Moreover, histological analysis revealed that feeding shrimp with cefotaxime at the given dose in once- or twice-a-day treatments did not negatively impact the shrimp hepatopancreas.

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Effect of oxytetracycline on the biosafety, gut microbial diversity, immune gene expression and withdrawal period in Pacific whiteleg shrimp, Penaeus vannamei

Cited by 9 publications

References 42 publications

Impacts of dietary supplementation of bamboo vinegar and charcoal powder on growth performance, intestinal morphology, and gut microflora of large-scale loach Paramisgurnus dabryanus

Impacts of dietary supplementation of bamboo vinegar and charcoal powder on growth performance, intestinal morphology, and gut microflora of large-scale loach Paramisgurnus dabryanus

Usage pattern of chemicals, biologicals and veterinary medicinal products in Indian aquaculture

Pharmacokinetics and Withdrawal Times of Cefotaxime in White Leg Shrimp (Litopenaeus vannamei) after Oral Administration

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