Effect of packaging conditions and temperature on viability of microencapsulated bifidobacteria during storage

Abstract

In this study, Bifidobacterium longum B6 and B infantis CCRC 14633 were microencapsulated in various wall materials, including skim milk, gum arabic, gelatin and soluble starch. The stability of these microencapsulated bifidobacteria held at 25 or 4 • C in glass or polyester bottles with or without deoxidant and desiccant was determined. Microencapsulated cells of B longum B6 were generally more stable than the corresponding microencapsulated cells of B infantis CCRC 14633 under the various storage conditions tested. The presence of deoxidant and desiccant, especially at 25 • C, increased the survival of microencapsulated cells. Furthermore, the survival of bifidobacteria was enhanced when they were stored at 4 • C in glass bottles. It was also found that the wall material affected the survival of microencapsulated bifidobacteria. The viability of B longum B6 and B infantis CCRC 14633 was best when they were encapsulated in skim milk and held at 4 • C in glass bottles. Skim milk-encapsulated B longum B6 cells showed a relatively low viability reduction of only 0.15-0.20 log (colony-forming units (cfu g −1 )) after 42 days of storage at 4 • C in glass bottles, regardless of the presence of deoxidant and desiccant. A reduction of 0.38-0.76 log (cfu g −1 ) was noted for skim milk-encapsulated cells of B infantis CCRC 14633 under similar storage conditions.

Supercritical CO2 interpolymer complex encapsulation improves heat stability of probiotic bifidobacteria

“…All samples were stored in glass vials, which could also have aided in shielding them from the negative effects of oxygen toxicity. Better survival of bifidobacteria stored in glass than polyester bottles was reported previously (Dave and Shah 1997;Hsiao et al 2004). Glass has also been shown to improve survival of bacteria when compared to poly (ethylene terepthalate) (PET) bottles (O'Riordan et al 2001).…”

“…Glass has also been shown to improve survival of bacteria when compared to poly (ethylene terepthalate) (PET) bottles (O'Riordan et al 2001). PET has higher oxygen permeability than glass (Ishibasi and Shimamura 1993; Hsiao et al 2004). However, since all samples in this study were stored in glass, improved probiotic viability can be attributed mainly to the protective effect of the interpolymer complex.…”

Technology and potential applications of probiotic encapsulation in fermented milk products

“…The assimilative reductions of cholesterol by nonencapsulated and encapsulated L. acidophilus ATCC 43,121 were 35.98 % and 32.84 %, respectively. Moreover, encapsulation did not significantly (P>0.05) affect the adherence of 10-20 μm (Lian et al, 2002;Lian et al, 2003;Hisiao et al 2004) L. acidophilus ATCC 43,121 onto the human intestinal epithelial cell lines HT-29. Studies suggest that microencapsulation of free probiotic cells can increase their viability by ≥2 log cycles in fermented milks during a refrigerated storage period.…”

“…It is well known that thermal and osmotic resistance of LAB is species-dependent. The survival of probiotics after spray drying also depends on the kinds and concentrations of carriers used as well as on the outlet temperature of the spray dryer (Ananta et al, 2005;Hisiao et al, 2004;Lian et al, 2003;Lian et al, 2002). Among different probiotic microorganisms, thermophilic or thermotolerant ones such as S. salivarius subsp.…”

Active and Intelligent Packaging Food - Research and Development – A Review

“…Kudelka [2005] analyzed the effect of pasteurization and package type on the acidity of probiotic yogurts made from goat's and cow's milk during 21 days refrigerated storage. Others studied the effect of the packaging material and the storage temperature on the viability of microencapsulated bifidobacteria [Hsiao et al, 2004].…”