Effect of pairing periods on mating and spawning success of the red swamp crayfish &lt;i&gt;Procambarus clarkii&lt;/i&gt; (Decapoda: Cambaridae) under laboratory conditions

Nishimoto, Sota; Hamasaki, Katsuyuki; Dan, Shigeki

doi:10.18353/crustacea.51.0_103

Cited by 3 publications

(1 citation statement)

References 23 publications

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“…Crayfish were cultured for mating and spawning at 25°C according to the methods detailed in our previous studies (Hamasaki et al, 2022;Nishimoto et al, 2022). We used embryos from three females spawned on 20 September 2019 (no.…”

Section: Crayfish and Test Embryosmentioning

confidence: 99%

Artificial incubation and hatching of embryos of the red swamp crayfish <i>Procambarus clarkii</i> (Decapoda: Cambaridae)

2023

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We studied the artificial incubation method of red swamp crayfish Procambarus clarkii embryos using cell culture plates. In the first experiment, cleavage stage embryos or egg-nauplius stage embryos were individually cultured at 20°C in the well of cell culture plates with different water volumes per well [12 well (4 ml), 24 well (2 ml), 48 well (1 ml) and 96 well (0.25 ml)]. In the second experiment, eggnauplius stage embryos were cultured using 12-well plates at 15, 20 and 25°C. Almost all cleavage stage embryos died without hatching. Egg-nauplius stage embryos survived to hatch. In the 96-well plates, the embryo hatched, but all first instar juveniles died soon. In other plates, the mortality of the first instar juveniles tended to increase with decreasing culture water volume; particularly, high mortality was recorded in the 48-well plates. Hatching and moulting rates were high at 15°C and 20°C, but approximately half of the first instar juveniles died at 25°C. We concluded that a 12-well cell culture plate (4 ml per well) is suitable for incubating P. clarkii embryos to hatch and the first instar juveniles to moult to the second instar at 15-20°C.

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Section: Crayfish and Test Embryosmentioning

confidence: 99%

Artificial incubation and hatching of embryos of the red swamp crayfish <i>Procambarus clarkii</i> (Decapoda: Cambaridae)

2023

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Reproductive biology of the red swamp crayfish Procambarus clarkii (Girard, 1852) (Decapoda: Astacidea: Cambaridae): A review

Hamasaki,

Dan,

Kawai

2023

Journal of Crustacean Biology

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The red swamp crayfish Procambarus clarkii (Girard, 1852) is an alien invasive species as well as a commercially exploited animal. The sterile-male-release technique (SMRT) using infertile but sexually active males has been an effective candidate measure to control this invasive crayfish. Additionally, the limited supply of juveniles due to females’ spawning seasonality hinders the further development of the P. clarkii aquaculture industry. Information on its reproductive biology, such mating strategies and factors affecting offspring production, is crucial for developing an effective SMRT and aquaculture systems for P. clarkii. We review the reproductive biology of this species in terms of 1) sexual dimorphism and reproductive status, 2) sex recognition, mating, and mate preferences, and 3) egg production, embryonic development, and hatching. Intra- and intersexual dimorphisms of chelae are evident in P. clarkii, and a reproductive morphotype (form I) with larger chelae and a non-reproductive morphotype (form II) with smaller chelae are detected in both females and males, allowing us to easily determine the reproductive status of both sexes. The crayfish exhibits mutual mate choice (i.e., preference for larger mates), and small-sized males reproductive potential is low. Female fitness (i.e., number of offspring) generally depends on their body size. Female brood care appears to promote early embryo development before the egg-nauplius stage. Further study is required to identify the maternal factors affecting early embryonic development, which may improve measures for controlling invasive populations and enhancing aquaculture productivity.

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Reproductive biology of the introduced red-swamp crayfish Procambarus clarkii (Girard, 1852) (Decapoda: Astacidea: Cambaridae) in western Japan

Luong,

Shiraishi,

Kawai

et al. 2023

Journal of Crustacean Biology

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The red swamp crayfish Procambarus clarkii (Girard, 1852) has been responsible for negative impacts on native benthic fauna and flora in invaded freshwater ecosystems around the world, including Japan. We need to clarify the reproductive biology in the invaded habitats as basic information to effectively control the introduced populations, but the reproductive biology of P. clarkii in Japan (especially in western Japan) has not been well studied. We conducted monthly samplings of P. clarkii from November 2015 to November 2016 in a pond (which does not freeze, even in winter) in Okayama, western Japan, both by using shrimp cage traps and by hand nets to examine aspects of the reproduction, including a form alternation (i.e., Form I and II). We also reared spawning females in the laboratory and calculated the accumulated water temperature during the period to Stage-3 juveniles after spawning. The total number of individuals caught throughout the study period was 6,319 (2,601 males and 2,777 females, with eight of unknown sex and 933 juveniles). The males were all Form I with a breeding status from September to November 2016. We first found ovigerous females in July 2016 and females carrying hatchlings in October 2016, even in January 2016. The form alternation in males was confirmed not only in the cheliped length, but in the hook length. In laboratory observations, approximately 52 days with approximately 1,222 °C degree-days of the accumulated water temperature were necessary to Stage-3 juveniles after spawning. Our results indicate that P. clarkii can reproduce within approximately five months of hatching.

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Effect of pairing periods on mating and spawning success of the red swamp crayfish <i>Procambarus clarkii</i> (Decapoda: Cambaridae) under laboratory conditions

Cited by 3 publications

References 23 publications

Artificial incubation and hatching of embryos of the red swamp crayfish <i>Procambarus clarkii</i> (Decapoda: Cambaridae)

Artificial incubation and hatching of embryos of the red swamp crayfish <i>Procambarus clarkii</i> (Decapoda: Cambaridae)

Reproductive biology of the red swamp crayfish Procambarus clarkii (Girard, 1852) (Decapoda: Astacidea: Cambaridae): A review

Reproductive biology of the introduced red-swamp crayfish Procambarus clarkii (Girard, 1852) (Decapoda: Astacidea: Cambaridae) in western Japan

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