Effect of pancreozymin on insulin and glucagon levels in blood and bile

Buchanan, KD; Je, Vance; Morgan, Anthony; Williams, RH

doi:10.1152/ajplegacy.1968.215.6.1293

Cited by 44 publications

(16 citation statements)

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“…The physiological significance of the inhibition of adenylate cyclase by glucagon in this subfraction is unclear. Glucagon and insulin are found in bile at a concentration similar to that in the portal vein (Buchanan et al, 1968) and insulin introduced into blood is eliminated 17min later in bile (Henderson, 1974). The inhibition of the adenylate cyclase by a polypeptide hormone, and the decreased insulin 13 binding observed at the bile canaliculus, may therefore form part of a control mechanism operating to prevent a delayed hormone response occurring.…”

Section: Bile-canicularfacementioning

confidence: 99%

Functional polarity of the rat hepatocyte surface membrane. Isolation and characterization of plasma-membrane subfractions from the blood-sinusoidal, bile-Canalicular and contiguous surfaces of the hepatocyte

Wisher¹,

Evans²

1975

Biochemical Journal

305

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1. Six rat liver plasma-membrane subfractions of different density and morphological, enzymric and chemical properties were prepared from homogenates by a combination of differential, rate-zonal and density-gradient centrifugation. They consisted of three vesicular 'light' subfractions of density 1.12-1.13 and three 'heavy' subfractions of density 1.16-1.18 containing membrane strips and intercellular junctions. 2. All six subfractions contained a basal adenylate cyclase activity. One of the 'light' subfractions that showed the highest glucagon-stimulated adenylate cyclase activity was identified as deriving from the blood-sinusoidal face of the hepatocyte. This subfraction, unlike the others, was contaminated by Golgi components, as indicated by its morphological properties and the presence of galactosyl-and sialyl-transferase activities. 3. All the six subfractions showed high activities of the following plasma-membrane marker enzymes: 5'-nucleotidase, alkaline phosphodiesterase (nucleotide pyrophosphatase), alkaline phosphatase, leucine naphthylamidase and Mg2+-activated adenosine triphosphatase. A 'light' subfraction that showed the highest specific activities of all the above marker enzymes, but lacked a glucagon-stimulated adenylate cyclase activity, was identified as deriving from the bilecanalicular face of the hepatocyte. 4. The 'heavy' subfractions, which showed generally the lowest activities of the above plasma-membrane enzyme markers, and were characterized by the presence of desmosomes and gap junctions, were taken to originate from the contiguous faces of the hepatocyte. 5. The protein composition of the six subfractions was generally similar, as shown by polyacrylamide-gel electrophoresis. Differences in the amounts of various protein and glycoprotein bands among the subfractions correlated with their morphology, enzymic composition and sialic acid content. 6. Hormonal and histochemical evidence supporting the identification of a bile-canalicular subfraction, a blood-sinusoidal subfraction and contiguous-face subfractions is discussed.

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Section: Bile-canicularfacementioning

confidence: 99%

Functional polarity of the rat hepatocyte surface membrane. Isolation and characterization of plasma-membrane subfractions from the blood-sinusoidal, bile-Canalicular and contiguous surfaces of the hepatocyte

Wisher¹,

Evans²

1975

Biochemical Journal

305

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“…This effect of CCK-PZ, either endogenous or exogenous, has not been reported in previous literature as far as we are aware. It is, however, not very much surprising, if we consider that the B cell is one of the targets of CCK-PZ and insulin is released from the cell by the action of this hormone (LINGER et al, 1967;MEADE et al, 1967;BUCHANAN et al, 1968;FUSSGANGER et al, 1969;DUPRE et al, 1969;OHARA, 1976). As in the acinar cell, CCK-PZ not only stimulates the secretory activity, but also causes mitotic division of the target cell.…”

Section: Endocrine Pancreasmentioning

confidence: 99%

Hypertrophy and Hyperplasia in the Endocrine and Exocrine Pancreas of Rats Fed Soybean Trypsin Inhibitor or Repeatedly Injected with Pancreozymin

Yanatori

Fujita

1976

Arch. Histol. Jap., Arch. Histol. Jpn

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Summary.Rats were 1) fed raw soybeans for 30 days, 2) given trypsin inhibitor as drinking water for 7 days, or 3) repeatedly injected with cholecystokinin-pancreozymin (CCK-PZ) for 7 days. After all these treatments the pancreas of the animals was markedly enlarged.Microscopic examination revealed, besides hypertrophy of acinar cells, increased mitotic figures in the acinar, centro-acinar, intercalated portion and excretory duct cells. The islets of Langerhans were found conspicuously enlarged due to mitotic proliferation of B cells. The endogenous CCK-PZ released by the intraluminal trypsin inhibitor and the exogenous CCK-PZ given by injections thus exerts not only secretagogous, but also trophic effects upon the endocrine and exocrine pancreas.The exocrine pancreas likely is affected also by the increased release of insulin from the B cells stimulated by CCK-PZ.

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“…Therefore, these three gastrointesinal hormones could be included in a gastrin group. According to the previous studies (Unger et al 1967;Buchanan et al 1968 ;Ohneda et al 1978), cholecystokinin-pancreozymin, gastrin or caerulein stimulates the release of pancreatic glucagon. Consequently, it is concluded from the present study that extrapancreatic glucagon seems to be enhanced by one of the gastrointestinal hormones of the gastrin family, similarly to the response of pancreatic glucagon.…”

Section: Discussionmentioning

confidence: 99%

Response of extrapancreatic glucagon to gastrointestinal hormones in pancreatectomized dogs.

Ohneda

Kobayashi

Nihei

1985

Tohoku J. Exp. Med.

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In order to investigate the effect of gastrointestinal hormones upon the secretion of extrapancreatic glucagon, tetragastrin, secretin, caerulein and cholecytokinin-pancreozymin octapeptide (CCK-octa) were administered during saline or arginine infusion in pancreatectomized dogs. Intravenous administration of tetragastrin (7 p g/kg) did not elicit any changes in plasma glucagon during saline infusion, while the plasma glucagon increased significantly following tetragastrin infusion during arginine infusion. The administration of secretin (3 U/kg) did not affect the plasma level of glucagon during saline or arginine infusion at all. Plasma glucagon did not change after the administration of caerulein (0.5pg/kg) during saline infusion, whereas it increased significantly following caerulein administration during arginine infusion. Intravenous administration of CCK-octa in a dose of 20 U/kg did not affect the plasma level of glucagon during saline infusion but exerted a significant rise of extrapancreatic glucagon during arginine infusion. It is concluded from the present experiment that the administration of tetragastrin, caerulein or CCK-octa enhances the release of extrapancreatic glucagon stimulated by arginine infusion while secretin infusion does not affect the secretion of extrapancreatic glucagon.tetragastrin ; secretin ; caerulein ; CCK-octapeptide ; arginine Since the establishment of the presence of extrapancreatic glucagon (Matsuyama and Foa 1974;Vranic et al. 1974), the regulation of extrapancreatic glucagon has been reported by many authors (Lefebvre and Luyckx 1981;Ohneda 1981). Among various factors, gastrointestinal factors have been considered to play an important role in the secretion of extrapancreatic glucagon. In a previous study from our laboratory it was demonstrated that extrapancreatic glucagon responds to intraluminal administration of glucose and arginine (Ohneda et al. 1984). Therefore, a participation of gastrointestinal hormones can be presumed in the regulation of extrapancreatic glucagon during nutrient ingestion. In this study, the effect of gastrointestinal hormones upon the secretion of extrapancreatic glucagon was investigated using pancreatectomized dogs.

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Effect of pancreozymin on insulin and glucagon levels in blood and bile

Cited by 44 publications

References 0 publications

Functional polarity of the rat hepatocyte surface membrane. Isolation and characterization of plasma-membrane subfractions from the blood-sinusoidal, bile-Canalicular and contiguous surfaces of the hepatocyte

Functional polarity of the rat hepatocyte surface membrane. Isolation and characterization of plasma-membrane subfractions from the blood-sinusoidal, bile-Canalicular and contiguous surfaces of the hepatocyte

Hypertrophy and Hyperplasia in the Endocrine and Exocrine Pancreas of Rats Fed Soybean Trypsin Inhibitor or Repeatedly Injected with Pancreozymin

Response of extrapancreatic glucagon to gastrointestinal hormones in pancreatectomized dogs.

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