Effect of Papaver rhoeas L. extract on in vitro maturation of sheep oocytes

Rajabi-Toustani, Reza; Motamedi-Mojdehi, Rasool; Mehr, Mohammad Roostaei-Ali; Motamedi-Mojdehi, Reihaneh

doi:10.1016/j.smallrumres.2013.06.010

Cited by 20 publications

(12 citation statements)

References 34 publications

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“…Among the concentrations used, 100.0 μg/mL promoted an increase in the maturation rate (70.7 vs. 56.3%) and developmental competence of embryos (34.8 vs. 18.8%) compared to those in control group. In addition, the P. rhoeas extract at concentration of 50.0 µg/mL also showed a positive effect on the maturation rate of sheep oocytes compared to the control group (70.1 vs. 54.7%) (RAJABI-TOUSTANI et al, 2013).…”

Section: Essential Oils and Extractsmentioning

confidence: 83%

Use of natural antioxidants in in vitro mammalian embryo production

Santos

Borges

Neta

et al. 2018

SCA

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In vitro embryo production (IVEP) contributes to the quantitative and qualitative aspects of animal reproduction. Nevertheless, inherent technical factors such as oxidative stress can negatively influence the result and this can impair cell metabolism, thus decreasing the rates of in vitro development, and necessitating the supplementation of culture medium with antioxidants. In this context, compounds of natural origin with this property have been highlighted because of the positive results obtained at different stages of IVEP. Thus, this review aims to present the results obtained by using natural antioxidants to minimize the effects of oxidative stress on gametes and embryos. A variety of natural isolated substances and mixtures (essential oils and extracts) have been studied for supplementation of IVEP media, at stages of in vitro maturation, sperm capacitation, in vitro fertilization, and in vitro development of embryos in different mammalian species. Generally, beneficial effects are observed according to the concentration used, thus demonstrating the potential of several natural antioxidants. Therefore, the main challenges in using these compounds as antioxidants during IVEP include proving their efficiency against free radicals and determining the best concentration at each stage. In addition, understanding the mechanisms of action of such antioxidants is crucial to establishing their use in IVEP biotechnology. Key words: Culture medium. Natural bioactive. Oxidative stress. Reproduction. ResumoA produção in vitro de embriões (PIVE) contribui para os aspectos quantitativos e qualitativos da reprodução animal. Contudo, fatores inerentes da técnica, como o estresse oxidativo, podem influenciar negativamente o resultado e isso pode prejudicar o metabolismo celular, diminuindo assim as taxas de desenvolvimento in vitro, e exigindo a suplementação do meio de cultivo com antioxidantes. Neste contexto, os compostos de origem natural com essa propriedade têm se destacado devido aos resultados positivos obtidos em diferentes estágios da PIVE. Assim, esta revisão pretende apresentar os resultados obtidos usando antioxidantes naturais para minimizar os efeitos do estresse oxidativo em gametas e embriões. Uma variedade de substâncias isoladas e de misturas naturais (óleos essenciais e extratos) tem sido estudada para a suplementação de meios de PIVE, nas etapas de maturação in vitro, capacitação espermática, fecundação in vitro e desenvolvimento in vitro de embriões em diferentes espécies de mamíferos. Geralmente, os efeitos benéficos são observados de acordo com a concentração utilizada,

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Section: Essential Oils and Extractsmentioning

confidence: 83%

Use of natural antioxidants in in vitro mammalian embryo production

Santos

Borges

Neta

et al. 2018

SCA

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“…It has been shown that the optimum concentration of Papaver rhoeas extract (100 µg/mL) in mouse oocyte maturation medium improves the rate of oocyte maturation and subsequently enhanced the rate of embryo development [28]. Rajabi-Toustani et al [16] showed that Papaver rhoeas extract enhanced maturation rate of follicls dependent on the extract concentration, so that P. rhoeas extract with an appropriate concentrations (50 µg/mL) in maturation medium elevated the sheep oocyte maturation rate, while the results of this investigation elucidated the beneficial effect of natural extracts such as sea cucumber as supplementation in IVM…”

Section: Discussionmentioning

confidence: 99%

“…The formation of reactive oxygen species (ROS) is a natural process that happens in the cell during electron transfer reactions. Cellular molecules such as lipids, proteins and nucleic acids are altered by ROS [5]. In the recent stud-ies different antioxidants such as ß-mercaptoethanol (ß-ME), cysteine, cysteamine [6] and anthocyanin were used to supplement IVM of oocyte [7].…”

Section: Introductionmentioning

confidence: 99%

“…In the recent stud-ies different antioxidants such as ß-mercaptoethanol (ß-ME), cysteine, cysteamine [6] and anthocyanin were used to supplement IVM of oocyte [7]. In the present time, there is an excessive interest in natural antioxidants instead of synthetic types, which may contribute to prevent oxidative damages [5].…”

Section: Introductionmentioning

confidence: 99%

“…It is indicated that during in vitro oocyte maturation, the levels of antioxidants have been reduced as compared with in vivo condition, therefore, the addition of a natural antioxidant to the medium may be appreciable for in vitro oocyte maturation [16].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Sea Cucumber Body Wall Extract Promoted in Vitro Maturation of NMRI Mice Follicles at Germinal Vesicle Stage

Khalilzadeh

Baharara

Jalali

et al. 2016

Zahedan J Res Med Sci

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Background: IVM (in vitro maturation) appear to be certified high pregnancy outcome. Therefore, attempt to find an appropriate culture system using natural products for increasing developmental competencies fascinating. Objectives: This experiment is aimed to evaluate the effect of sea cucumber methanol extract on in vitro maturation of immature mouse oocyte. Methods: In this experimental study, Preantral follicles at germinal vesicle stage were collected from 24-26 days NMRI female mouse ovaries and transferred to IVM medium supplemented with different concentration of sea cucumber methanol extract (5, 10, 20, 30 µg/mL). Antioxidant capacity of sea cucumber extract was evaluated using DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay. Further, oocyte maturation and TNF-α expression were recorded till day 10 th. Data were analyzed by one-way analysis of variance (ANOVA) through SPSS 16. Results: The percentage of arrested oocyte at germinal vesicle stage in the control group was significantly (P < 0.05) higher than treated group (20 µg/mL). There were significant (P < 0.01) differences in percentage of maturated oocyte to metaphase II (MII) stage between treated and control group. DPPH radical scavenging capacity and reduction of TNF-α expression in treated oocyte demonstrated that sea cucumber increased rate of oocyte maturation. In addition, the treated oocyte (20 µg/mL) achieved the highest percentage of MII stage. Conclusions: It was concluded that the sea cucumber extract with optimum concentration can improve oocyte maturation. Sea cucumber extract treatment can be suggested as a novel therapeutic strategy to be used in infertility modality in the future.

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First pregnancy after in vitro culture of early antral follicles in goats: Positive effects of anethole on follicle development and steroidogenesis

Sá

Ferreira

Sousa

et al. 2020

Molecular Reproduction Devel

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This study aimed to evaluate the role of anethole during the in vitro culture of caprine early antral follicles. Early antral follicles were isolated from caprine ovaries and cultured for 18 days without (control) or with anethole (300 µg/ml). After culture, the cumulus-oocyte complexes were subjected to in vitro maturation, followed by parthenogenetic activation or in vitro fertilization (IVF) and embryo culture. Follicular walls were used for the quantification of messenger RNA (mRNA) of CYP19A1, CYP17, MMP-9, TIMP-2, Bax, and Bcl-2 genes, and culture medium was used for evaluation of ferric reducing antioxidant power (FRAP) and estradiol levels. After in vitro follicle culture (IVFC), anethole induced higher total antioxidant capacity, that is, it produced higher FRAP levels, reduced the Bax/Bcl-2 ratio, and increased the levels of mRNA for CYP19A1 and CYP17, which was associated with a greater estradiol production (p < .05). Also, anethole improved the ability of oocytes to resume meiosis and reach metaphase II stage, as well as yielded higher (p < .05) embryo production (e.g., morulas and blastocysts) in both parthenogenetic activation and IVF techniques. One pregnancy (Day 30) was obtained from IVFC with anethole. In conclusion, anethole promoted in vitro growth and maturation of goat early antral follicles and oocytes and enabled embryo production. Furthermore, this study reports, for the first time in goats, a pregnancy after IVF using oocytes originated from early antral follicles grown in vitro. K E Y W O R D S anethole, antral follicle, FRAP, goat, oocyte and embryo How to cite this article: de Sá NAR, Ferreira ACA, Sousa FGC, et al. First pregnancy after in vitro culture of early antral follicles in goats: Positive effects of anethole on follicle development and steroidogenesis. Mol Reprod Dev.

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Effect of Papaver rhoeas L. extract on in vitro maturation of sheep oocytes

Cited by 20 publications

References 34 publications

Use of natural antioxidants in in vitro mammalian embryo production

Use of natural antioxidants in in vitro mammalian embryo production

The Sea Cucumber Body Wall Extract Promoted in Vitro Maturation of NMRI Mice Follicles at Germinal Vesicle Stage

First pregnancy after in vitro culture of early antral follicles in goats: Positive effects of anethole on follicle development and steroidogenesis

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