Effect of parenteral immunization on the local immunoglobulin A response of the intestine to Shigella flexneri antigens

Keren, David F.; Scott, Patrícia; McDonald, Roderick A.; Wiatrak, M

doi:10.1128/iai.42.1.202-207.1983

Cited by 13 publications

(6 citation statements)

References 15 publications

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“…In all of the above groups, no or trivial amounts of IgG anti-shigella LPS were found in either serum or intestinal secretions. This lack of IgG in intestinal secretions after oral immunization was consistent with our previous studies (15,16) and is not due to the instability of IgG in the isolated ileal loops (19 Shedding of live bacteria. Loop fluids and stool samples were cultured on MacConkey agar plates to detect shedding of shigellae as described previously (17).…”

Section: Resultssupporting

confidence: 92%

“…A previous study from our laboratory has indicated that to achieve an enhanced local IgA memory response to Shigella flexneri, oral immunization is effective, but parenteral immunization (without adjuvant) is not (19). Others have found that parenteral immunization may actually suppress the subsequent local immune response to the same antigen (20,21).…”

Section: Discussionmentioning

confidence: 99%

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Effect of antigen form on local immunoglobulin A memory response of intestinal secretions to Shigella flexneri

Keren¹,

McDonald²,

Scott³

et al. 1985

Infect Immun

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An enhanced memory response, as shown by increased titers of specific immunoglobulin A (IgA), was seen in intestinal secretions from isolated Thiry-Vella loops in rabbits primed orally with live, locally invasive Shigella sp. X16 and challenged 60 days later with a single oral dose of the same antigen. Heat-killed shigella preparations, when used as either the priming or challenge antigen, did not elicit such a memory response in this system. In the present study, the role of antigen form and dosage in eliciting the enhanced local IgA response was investigated. A noninvasive strain, Shigellaflexneyi 2457-0, was capable of significantly enhancing the mucosal IgA memory response, whereas heat-killed Shigella sp. X16 was unable to augment the local IgA response, even when the priming dose was increased 100-fold. A proposed mucosal adjuvant, DEAE-dextran, given orally with live Shigella sp. X16, did not enhance the local IgA response. Viable, noninvasive shigellae were effective priming agents in enhancing the local IgA memory response. The poor mucosal response to heat-killed shigella preparations is thought to be related to an ineffective delivery of nonviable bacterial antigens into gut-associated lymphoid tissues. The ability of the live, noninvasive strain to elicit a vigorous local IgA memory response when given orally to rabbits was consistent with previous findings that live preparations elicit the best mucosal IgA response.

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Section: Resultssupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Effect of antigen form on local immunoglobulin A memory response of intestinal secretions to Shigella flexneri

Keren¹,

McDonald²,

Scott³

et al. 1985

Infect Immun

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“…Some workers have employed combinations of parenteral injections of antigen and oral stimulation (6,20,29). Most often, priming of animals by parenteral immunization several days to weeks before mucosal stimulation has been ineffec-tive in enhancing mucosal immunity (17,40). A notable exception is the use of intraperitoneal priming, which probably causes some direct stimulation of mucosal immunity (26,27).…”

mentioning

confidence: 99%

Combined parenteral and oral immunization results in an enhanced mucosal immunoglobulin A response to Shigella flexneri

1988

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Achieving a vigorous secretory immunoglobulin A (IgA) response in intestinal secretions usually requires multiple doses of antigen given orally, while systemic immunity is more easily attained by parenteral immunization. This study examines the role of combined parenteral and oral immunizations to enhance the early mucosal immune response to an enteropathogen. We have used a chronically isolated intestinal-loop model in rabbits as a probe to monitor kinetically the initial (primary) local immune response to shigella lipopolysaccharide (LPS) following combinations of parenteral immunization intramuscularly (i.m.) and oral stimulation with shigellae. Predictably, effective stimulation of systemic immunity was elicited when heat-killed preparations of Shigella sp. strain X16 were given i.m., as shown by strong serum IgG and weak intestinal IgA activity to shigella LPS. A single oral dose of live Shigella sp. strain X16 given to unprimed rabbits elicited only a typical weak IgA response in intestinal secretions. However, when an i.m. dose of heat-killed shigellae was followed 1 day later by an oral dose of live Shigella sp. strain X16, a hyperstimulation of the early secretory IgA response was elicited, and the response reached levels found previously only after multiple oral administrations of live shigellae. This stimulation did not require the use of an adjuvant. At the same time, the animals receiving this combined oral and i.m. regimen had a lower IgG antishigella LPS activity in serum compared with their response after receiving parenteral antigen in adjuvant alone. These findings indicate that while a dichotomy exists between the systemic and mucosal immune responses, careful orchestration of the stimulatory events can promote a vigorous early local IgA response.

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“…The presence of specific IgG immunoglobulins and the lack of specific IgA immunoglobulins in the milk of vaccinated dams was rather unexpected since parenteral immunization with other enteric organisms had previously led to production of a specific IgA as well as IgG response (Keren et al 1983). The ELISA assay used in this study detected low amounts of specific IgA immunoglobulins in the positive control wells, which seems to indicate a true absence of specific IgA immunoglobulins in the milk of vaccinated dams.…”

Section: Discussionmentioning

confidence: 51%

Comparison of different vaccines and induced immune response againstCampylobacter jejunicolonization in the infant mouse

1989

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The degree of protection conferred by vaccinated dams on infant mice against colonization by Campylobacter jejuni depended on the bacterial strain, preparation, and route of administration of the vaccine. In some instances of homologous protection, serum bactericidal titres correlated well with protection. However, boiled C. jejuni vaccine, which was non-protective, also elicited a strong bactericidal antibody response. Conversely, bactericidal activity could not be demonstrated against strains capable of cross-protection. There was a good correlation between high campylobacter-specific IgG response and bactericidal activity.

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Effect of parenteral immunization on the local immunoglobulin A response of the intestine to Shigella flexneri antigens

Cited by 13 publications

References 15 publications

Effect of antigen form on local immunoglobulin A memory response of intestinal secretions to Shigella flexneri

Effect of antigen form on local immunoglobulin A memory response of intestinal secretions to Shigella flexneri

Combined parenteral and oral immunization results in an enhanced mucosal immunoglobulin A response to Shigella flexneri

Comparison of different vaccines and induced immune response againstCampylobacter jejunicolonization in the infant mouse

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