Effect of Particle Size of Nanospheres and Microspheres on the Cellular-Association and Cytotoxicity of Paclitaxel in 4T1 Cells

Abstract

Cell-association of paclitaxel increased in 4T1, Caco-2, and Cor-L23/R as particle size increased. Paclitaxel delivered from 1-mum microspheres was thrice more cytotoxic to 4T1 cells compared to the drug delivered from nanospheres or solution.

The behavior of lipid debris left on cell surfaces from microbubble based ultrasound molecular imaging

“…It is unlikely that the fragments were endocytosed by the cell because the large fragment sizes of 3 – 6 μm are difficult for these cells to endocytose. [37]…”

The Anti-Melanoma Efficiency of the Intratumoral Injection of Cucurbitacin-Loaded Sustained-Release Carriers: A PLGA Particle System

“…Chakravarthi and coworkers13,19,20 reported that small particles (∼1 μm) could be adsorbed onto the cell surface and thus enhance the therapeutic efficiency. In our studies, we noted that the fluorescent probe and the microparticles did not interact with the cells (the pictures are not shown).…”

“…As the endocytosis for NPs was inevitable and the particle size of the NPs was in the range of 200-400 nm in our experiments, the possible mechanism of the limited endocytosis may be attributed to the macropinocytosis. 42 Chakravarthi and coworkers 13,19,20 reported that small particles (∼1 :m) could be adsorbed onto the cell surface and thus enhance the therapeutic effi-ciency. In our studies, we noted that the fluorescent probe and the microparticles did not interact with the cells (the pictures are not shown).…”

“…A surprisingly small subset of these technologies has demonstrated potentially curative preclinical results for cancer applications, and fewer have progressed toward commercialization 12. Chakravarthi and cowokers13,19 studied the paclitaxel‐loaded PLGA particles of different sizes to treat the breast cancer, and the particles of about 1 μm showed better efficiencies after intratumoral injection to 4T1 cell‐bearing mouse, which was suggested to be mainly attributed to the particles–cells interactions 20…”

Nanomedicine of anastrozole for breast cancer: Physicochemical evaluation, in vitro cytotoxicity on BT-549 and MCF-7 cell lines and preclinical study on rat model

“…The less negative value of the other systems (PLA and PCL) was due to the higher molecular weight of polymers. Increase in polydispersity indicates poor stability of the dispersion as observed by the presence of turbidity [30]. Overall, the negative charge leads to strong repellent interactions among the nanoparticles (ANP-10, ANL-10 and ANC-10) in dispersion and thus high stability, did not aggregate upon storage for longer period.…”