Effect of Passage Number of Conditioned Medium Collected from Equine Amniotic Fluid Mesenchymal Stem Cells: Porcine Oocyte Maturation and Embryo Development

Park, Ahyoung; Oh, Hyun Ju; Ji, Kuk-Bin; Choi, Eunha Miri; Kim, Dongern; Kim, Eun Young; Kim, Min Kyu

doi:10.3390/ijms23126569

Cited by 8 publications

(5 citation statements)

References 59 publications

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“…The normal development of early embryos serves as the primary indicator in the assessment of the cytoplasmic maturation of oocytes and is an important criterion for evaluating oocyte quality [33][34][35][36]. Our study demonstrated a significant improvement in the cleavage rates and blastocyst formation rates of embryos that were subjected to subsequent IVF and SCNT following FA supplementation.…”

Section: Discussionmentioning

confidence: 58%

Ferulic Acid Enhances Oocyte Maturation and the Subsequent Development of Bovine Oocytes

Wang,

Qi,

Yin

et al. 2023

IJMS

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Improving the quality of oocytes matured in vitro is integral to enhancing the efficacy of in vitro embryo production. Oxidative stress is one of the primary causes of quality decline in oocytes matured in vitro. In this study, ferulic acid (FA), a natural antioxidant found in plant cell walls, was investigated to evaluate its impact on bovine oocyte maturation and subsequent embryonic development. Bovine cumulus–oocyte complexes (COCs) were treated with different concentrations of FA (0, 2.5, 5, 10, 20 μM) during in vitro maturation (IVM). Compared to the control group, supplementation with 5 μM FA significantly enhanced the maturation rates of bovine oocytes and the expansion of the cumulus cells area, as well as the subsequent cleavage and blastocyst formation rates after in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT). Furthermore, FA supplementation was observed to effectively decrease the levels of ROS in bovine oocytes and improve their mitochondrial function. Our experiments demonstrate that FA can maintain the levels of antioxidants (GSH, SOD, CAT) in oocytes, thereby alleviating the oxidative stress induced by H2O2. RT-qPCR results revealed that, after FA treatment, the relative mRNA expression levels of genes related to oocyte maturation (GDF-9 and BMP-15), cumulus cell expansion (HAS2, PTX3, CX37, and CX43), and embryo pluripotency (OCT4, SOX2, and CDX2) were significantly increased. In conclusion, these findings demonstrate that FA supplementation during bovine oocyte IVM can enhance oocyte quality and the developmental potential of subsequent embryos.

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Section: Discussionmentioning

confidence: 58%

Ferulic Acid Enhances Oocyte Maturation and the Subsequent Development of Bovine Oocytes

Wang,

Qi,

Yin

et al. 2023

IJMS

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“…Cancer cells during the cell metabolism usually excrete various metabolites into the intracellular media. Among them are GSH and other products that may play the role of reducing agents [ 45 , 46 ]. Therefore, when redox-responsive nanoparticles are added to the cancer cell medium, the metabolites can cause some reduction processes in their disulfide sites, and even aggregation.…”

Section: Resultsmentioning

confidence: 99%

“…This might be due to partial aggregation of the MNP-SS in the intercellular medium containing cell metabolites, some of which can be relatively active reducing agents, such as GSH, cysteine, etc. [ 45 , 46 ]. Tests on the colloidal stability in the conditioned medium showed that the MNP-SS partially aggregates and becomes more polydispersed ( Figure 3 D).…”

Section: Resultsmentioning

confidence: 99%

Study of Cytotoxicity and Internalization of Redox-Responsive Iron Oxide Nanoparticles on PC-3 and 4T1 Cancer Cell Lines

et al. 2022

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Redox-responsive and magnetic nanomaterials are widely used in tumor treatment separately, and while the application of their combined functionalities is perspective, exactly how such synergistic effects can be implemented is still unclear. This report investigates the internalization dynamics of magnetic redox-responsive nanoparticles (MNP-SS) and their cytotoxicity toward PC-3 and 4T1 cell lines. It is shown that MNP-SS synthesized by covalent grafting of polyethylene glycol (PEG) on the magnetic nanoparticle (MNP) surface via SS-bonds lose their colloidal stability and aggregate fully in a solution containing DTT, and partially in conditioned media, whereas the PEGylated MNP (MNP-PEG) without S-S linker control remains stable under the same conditions. Internalized MNP-SS lose the PEG shell more quickly, causing enhanced magnetic core dissolution and thus increased toxicity. This was confirmed by fluorescence microscopy using MNP-SS dual-labeled by Cy3 via labile disulfide, and Cy5 via a rigid linker. The dyes demonstrated a significant difference in fluorescence dynamics and intensity. Additionally, MNP-SS demonstrate quicker cellular uptake compared to MNP-PEG, as confirmed by TEM analysis. The combination of disulfide bonds, leading to faster dissolution of the iron oxide core, and the high-oxidative potential Fe3+ ions can synergically enhance oxidative stress in comparison with more stable coating without SS-bonds in the case of MNP-PEG. It decreases the cancer cell viability, especially for the 4T1, which is known for being sensitive to ferroptosis-triggering factors. In this work, we have shown the effect of redox-responsive grafting of the MNP surface as a key factor affecting MNP-internalization rate and dissolution with the release of iron ions inside cancer cells. This kind of synergistic effect is described for the first time and can be used not only in combination with drug delivery, but also in treatment of tumors responsive to ferroptosis.

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“…Despite advances in culture conditions, media and protocols for oocyte maturation and embryo development, in vitro outcomes are still far from desirable compared with embryos produced in vivo ( 19 , 20 ). In particular, recent studies have shown the beneficial effect of co-culture with MSCs ( 21 ), oviduct cells, and cumulus cells ( 22 , 23 ) on the development of oocytes and embryos in various mammalian species, including cattle ( 24 ), horses ( 25 ), pigs ( 26 ) and canines ( 27 ). Therefore, culture conditions are crucial in determining the quality of in vitro -produced embryos.…”

Section: Discussionmentioning

confidence: 99%

Feline Wharton’s jelly-derived mesenchymal stem cells as a feeder layer for oocytes maturation and embryos culture in vitro

Baouche,

Ochota,

Mermillod

et al. 2023

Front. Vet. Sci.

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IntroductionDue to their capacity to release growth factors and cytokines, co-culture using mesenchymal stem cells has been considered a good alternative to promoting the maturation of the oocytes and the embryo’s development quality in vitro in different mammalian species. In this regard, we investigated the effect of feline Wharton’s jelly MSCs as feeders layer in oocyte maturation—consequently, the development of resulting embryos in co-culture.MethodsOocytes with dark cytoplasm and a few layers of cumulus cells were collected and subjected to in vitro maturation and embryo culture using commercial media with and without MSCs addition. The oocytes’ nuclear maturation and the degree of cumulus expansion in different groups were assessed after 24 h; the development of the embryo was evaluated every 12 h until day eight.ResultsAlthough MSCs increased the proportion of cumulus cells oocytes exhibiting cumulus expansion, there were no significant differences in the percentage of matured oocytes (metaphase II) among the groups (p > 0.05). However, the embryo development differs significantly, with a higher cleavage, morula, and blastocyst percentage in oocytes matured with MSC co-culture conditions than in commercial media alone (p < 0.05). Also, we observed higher morula and blastocyst rates in the embryos co-cultured with MSCs during the in vitro culture (p > 0.05).ConclusionBased on our results, the co-culture with MSCs during the oocyte maturation resulted in better embryo development, as well as the MSCs addition during embryo culture returned an increased number of morula and blastocysts. Further research is needed to fully understand and optimize the use of MSCs in oocyte maturation and embryo development.

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Effect of Passage Number of Conditioned Medium Collected from Equine Amniotic Fluid Mesenchymal Stem Cells: Porcine Oocyte Maturation and Embryo Development

Cited by 8 publications

References 59 publications

Ferulic Acid Enhances Oocyte Maturation and the Subsequent Development of Bovine Oocytes

Ferulic Acid Enhances Oocyte Maturation and the Subsequent Development of Bovine Oocytes

Study of Cytotoxicity and Internalization of Redox-Responsive Iron Oxide Nanoparticles on PC-3 and 4T1 Cancer Cell Lines

Feline Wharton’s jelly-derived mesenchymal stem cells as a feeder layer for oocytes maturation and embryos culture in vitro

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