Effect of PCL/nHAEA nanocomposite to osteo/odontogenic differentiation of dental pulp stem cells

Azaryan, Ehsaneh; Hanafi‐Bojd, Mohammad Yahya; Alemzadeh, Esmat; Razavi, Fariba Emadian; Naseri, Mohsen

doi:10.1186/s12903-022-02527-1

Cited by 12 publications

(13 citation statements)

References 40 publications

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“…Scaffold biomaterials for successful tooth regeneration applications should have some requirements such as being biocompatible, biodegradable, and possess mechanical properties that are consistent with the implanted area as well as being used in the appropriate amount and with an accessible volume of porosities for the diffusion of oxygen, cells, and nutrients [ 6 , 7 ]. To date, many polymeric materials have been reported to create biodegradable scaffolds for dental tissue engineering including poly(lactide) (PLA) [ 8 ], poly(lactide-co-glycolide) (PLGA) [ 9 , 10 ], and polycaprolactone (PCL) [ 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Microbial Poly(hydroxybutyrate-co-hydroxyvalerate) Scaffold for Periodontal Tissue Engineering

et al. 2023

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In this study, we fabricated three dimensional (3D) porous scaffolds of poly(hydroxybutyrate-co-hydroxyvalerate) with 50% HV content. P(HB-50HV) was biosynthesized from bacteria Cupriavidus necator H16 and the in vitro proliferation of dental cells for tissue engineering application was evaluated. Comparisons were made with scaffolds prepared by poly(hydroxybutyrate) (PHB), poly(hydroxybutyrate-co-12%hydroxyvalerate) (P(HB-12HV)), and polycaprolactone (PCL). The water contact angle results indicated a hydrophobic character for all polymeric films. All fabricated scaffolds exhibited a high porosity of 90% with a sponge-like appearance. The P(HB-50HV) scaffolds were distinctively different in compressive modulus and was the material with the lowest stiffness among all scaffolds tested between the dry and wet conditions. The human gingival fibroblasts (HGFs) and periodontal ligament stem cells (PDLSCs) cultured onto the P(HB-50HV) scaffold adhered to the scaffold and exhibited the highest proliferation with a healthy morphology, demonstrating excellent cell compatibility with P(HB-50HV) scaffolds. These results indicate that the P(HB-50HV) scaffold could be applied as a biomaterial for periodontal tissue engineering and stem cell applications.

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Section: Introductionmentioning

confidence: 99%

Microbial Poly(hydroxybutyrate-co-hydroxyvalerate) Scaffold for Periodontal Tissue Engineering

et al. 2023

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“…116 Recently, a new approach in material synthesis has been made possible by the green synthesis of nanoparticles. 115 According to a study conducted by our group, nanohydroxyapatite (nHA) synthesized via EA extract (nHAEA) demonstrated the capacity to modulate genes involved in dentin-pulp regeneration and immunomodulation in DPSCs. 80 Furthermore, we evaluated the effect of PCL/nHAEA nanofibers on DPSCs' ability to differentiate into odontogenic cells.…”

Section: Elaeagnus Angustifoliamentioning

confidence: 99%

“…Our findings will provide the groundwork for future cell-based dentin regeneration treatments utilizing PCL/nHAEA nanomaterials. 115…”

Section: Elaeagnus Angustifoliamentioning

confidence: 99%

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Dentin regeneration based on tooth tissue engineering: A review

Azaryan

Razavi

Hanafi‐Bojd

et al. 2023

Biotechnology Progress

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Missing or damaged teeth due to caries, genetic disorders, oral cancer, or infection may contribute to physical and mental impairment that reduces the quality of life.Despite major progress in dental tissue repair and those replacing missing teeth with prostheses, clinical treatments are not yet entirely satisfactory, as they do not regenerate tissues with natural teeth features. Therefore, much of the focus has centered on tissue engineering (TE) based on dental stem/progenitor cells to create bioengineered dental tissues. Many in vitro and in vivo studies have shown the use of cells in regenerating sections of a tooth or a whole tooth. Tooth tissue engineering (TTE), as a promising method for dental tissue regeneration, can form durable biological substitutes for soft and mineralized dental tissues. The cell-based TE approach, which directly seeds cells and bioactive components onto the biodegradable scaffolds, is currently the most potential method. Three essential components of this strategy are cells, scaffolds, and growth factors (GFs). This study investigates dentin regeneration after an injury such as caries using TE and stem/progenitor cell-based strategies. We begin by discussing about the biological structure of a dentin and dentinogenesis.The engineering of teeth requires knowledge of the processes that underlie the growth of an organ or tissue. Then, the three fundamental requirements for dentin regeneration, namely cell sources, GFs, and scaffolds are covered in the current study, which may ultimately lead to new insights in this field.

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“…Mesenchymal stem cells (MSCs) are multipotent stem cells found in a variety of adult tissues, including bone marrow, adipose tissue, and tooth pulp. These cells are distinguished by their ability to self-renew and multidifferentiate [16][17][18]. MSCs have a high paracrine influence on the body.…”

Section: Introductionmentioning

confidence: 99%

Effect of HM-Exos on the migration and inflammatory response of LPS-exposed dental pulp stem cells

et al. 2023

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Aim The purpose of this study was to investigate the effects of human milk exosomes (HM-Exos) on the viability, migration, and inflammatory responses of lipopolysaccharide (LPS)-exposed human dental pulp stem cells (HDPSCs) in vitro. Methods HM-Exos were isolated, and dynamic light scattering (DLS), scanning electron microscopy (SEM), and transmission electron microscopy (TEM) were used to analyze their physical properties (size and shape). To construct an in vitro inflammation model, HDPSCs were exposed to LPS. The MTT test and migration assay were used to investigate the effect of HM-Exos on cell proliferation and migration, and the quantitative polymerase chain reaction (qPCR) was used to assess the expression of inflammatory genes in HDPSCs. Data were analyzed using a one-way analysis of variance (ANOVA) with Tukey's post-test. Results DLS measurement revealed that HM-Exos were 116.8 ± 3.6 nm in diameter. The SEM and TEM images revealed spherical shapes with diameters of 97.2 ± 34.6 nm. According to the results of the cell viability assay, the nontoxic concentration of HM-Exos (200 µg/ml) was chosen for the subsequent investigations. The migration assay results showed that HM-Exos improved the potential of LPS-exposed HDPSCs to migrate. The qPCR results indicated that HM-Exos significantly reduced the expression of inflammatory cytokines such as TNF-α, IL-1β, and IL-6 in HDPSCs after LPS stimulation. Conclusions HM-Exos increased LPS-exposed HDPSCs migration and proliferation and reduced gene expression of inflammatory cytokines. They may be a viable candidate for pulpitis therapy.

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Effect of PCL/nHAEA nanocomposite to osteo/odontogenic differentiation of dental pulp stem cells

Cited by 12 publications

References 40 publications

Microbial Poly(hydroxybutyrate-co-hydroxyvalerate) Scaffold for Periodontal Tissue Engineering

Microbial Poly(hydroxybutyrate-co-hydroxyvalerate) Scaffold for Periodontal Tissue Engineering

Dentin regeneration based on tooth tissue engineering: A review

Effect of HM-Exos on the migration and inflammatory response of LPS-exposed dental pulp stem cells

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