Effect of pentobarbital or urethane on bile secretion and chemical composition of blood in the rabbit

Abstract

SummaryA study was made of the effects of anaesthesia with pentobarbital and urethane on the bile secretion and the chemical composition of the blood of New Zealand rabbits. Neither of the agents was observed to affect arterial pH or p02' but with urethane pC02 values decreased significantly. This was assoeiated with a pronounced hyperglycaemia. Bile flow was significantly higher in pentobarbital-anaesthetized animals than in urethane-anaesthetized animals, a phenomenon that can be attributed to a different canalicular flow and that will not be related to differences in the bile-aeid-dependent fraction of secretioQ. Under both anaesthetics, bile sodium concentrations were greater than those found in plasma, which can be explained by the formation of micelles with low osmotic activity. Bile bicarbonate concentrations proved to be greater than those observed in plasma. Plasma caleium concentrations were significantly lower with urethane than with pentobarbital, whereas in bile the situation was reversed; both these aspects are discussed.Keywords: Anaesthesia, general; Pentobarbital; Urethane; Bile; Blood; Rabbits Anaesthetics have several effects on the structure and functioning of numerous organs and biological systems and such effects have been the subject of diverse research work; the possible modifications that these compounds may induce in biliary secretion have received little attention, however. This is rather paradoxical in view of the fact that numerous experiments in hepatic physiology and

Using quantitative intravital multiphoton microscopy to dissect hepatic transport in rats

“…One concession that most investigators make is to use general anesthetics, which themselves can affect hepatic transport [73,74] and potentially introduce drug-drug interactions that complicate studies.…”

The effect of diethyl ether, pentobarbitone and urethane anaesthesia on diflunisal conjugation and disposition in rats

“…Pentobarbitone and its metabolites are excreted extensively into bile and exert a choleretic effect (Klaassen 1971, Kuipers et al 1985, Collado et al 1987. Biliary secretion of succinylsulphathiazole (Bailey et al 1979 (Cooke and Cooke 1983), indocyanine green and S( -)-acenocoumarol (Daemen et al 1986) were decreased under pentobarbitone anaesthesia, raising the possibility of competition between pentobarbitone and other administered compounds (and their metabolites) for available hepatobiliary transport systems.…”

Suplementação com caldo de cana de açucar comparado com outros carboidratos na reposição do glicogenio e cinetica de biomarcadores pos-exercicio agudo em ratos

“…DiscussãoOs resultados apresentados nesse estudo mostraram a comparação de diferentes anestésicos em ratos Wistar em relação ao grau de hemólise e concentrações de glicogênio muscular e hepático após anestesia profunda. Embora a anestesia possa modificar respostas bioquímicas, metabólicas e hormonais(BUSH, 1991; GONZÁLEZ GIL, 2005), poucos estudos têm investigado o efeito de diferentes anestésicos sobre esses parâmetros simultaneamente em animais(COLLADO, 1987; GONZÁLEZ GIL, 2005).Existem vários estudos na literatura comparando os efeitos cardiovasculares de diferentes anestésicos(SAHA, 2007). Porém, poucos analisam os efeitos em parâmetros bioquímicos de animais.OO anestésico KX produziu um grau de hemólise menos acentuado quando comparado ao CH, mas em contrapartida apresentou um potente efeito glicogenolítico hepático e muscular.…”