Effect of peroxisome proliferation on ether phospholipid biosynthesizing enzymes in rat liver

Hardeman, D.; Zomer, Hanneke W.M.; Schutgens, R. B. H.; Tager, J. M.; Bosch, H. van den

doi:10.1016/0020-711x(90)90231-q

Cited by 23 publications

(17 citation statements)

References 34 publications

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“…Indeed, ADAPS might constitute a new PPARg-dependent gene. Although ADAPS is exclusively localized in peroxisomes (Singh et al, 1993) it is not induced by peroxisome proliferators, known PPARa activators (Hardeman et al, 1990), suggesting that it is not a PPARa-target gene. Further, PPARg agonists also up-regulate ADAPS in B12 oligodendrocyte-like cells .…”

Section: Discussionmentioning

confidence: 98%

A PPARs cross‐talk concertedly commits C6 glioma cells to oligodendrocytes and induces enzymes involved in myelin synthesis

Leisewitz

Urrutia

Martinez

et al. 2008

Journal Cellular Physiology

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Peroxisome proliferator activated receptors (PPARs, alpha, beta/delta, gamma) control lipid homeostasis and differentiation in various tissues and tumor cells. PPARbeta and PPARgamma increase oligodendrocyte maturation in glial mixed populations and spinal cord oligodendrocytes, respectively, and PPARbeta is known to modulate the activity of other PPARs. To assess a possible interaction between PPARs in glial cell differentiation we used the undifferentiated C6 glioma cell line as model. These cells express all three PPARs, but only PPARgamma shows transcriptional activity in agonist-based reporter gene assay. Agonist-activated PPARgamma up-regulates oligodendrocyte markers, down-regulates an astrocyte marker, and increases alkyl-dihydroxyacetone phosphate synthase, enzyme involved in the synthesis of myelin-rich plasmalogens. Similar effects are induced in PPARgamma overexpressing cells, which in addition show PPARbeta up-regulation. PPARbeta or PPARalpha agonists show no effect. Nevertheless, PPARbeta overexpression up-regulates PPARgamma and commits C6 cells to oligodendrocytes; effect that is abrogated by a PPARgamma antagonist or PPARgamma interference RNA. Moreover, PPARbeta overexpression also induces PPARalpha and its target genes, including acyl-CoA oxidase, enzyme involved in very long chain fatty acid recycling, and in the synthesis of myelin components such as docosahexaenoic acid. These results indicate for the first time, that PPARs concertedly cooperate in C6 glioma cell differentiation to oligodendrocytes. Further, they suggest that active PPARbeta might be essential for increasing oligodendrocyte distinctive markers and enzymes required for myelin synthesis in C6 glioma cells through up-regulation of PPARgamma and PPARalpha.

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Section: Discussionmentioning

confidence: 98%

A PPARs cross‐talk concertedly commits C6 glioma cells to oligodendrocytes and induces enzymes involved in myelin synthesis

Leisewitz

Urrutia

Martinez

et al. 2008

Journal Cellular Physiology

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“…In homogenates the specific activity of alkyl-DHAP synthase was hardly affected by proliferation (205,206), and a slight decrease in purified peroxisomes was observed (206) . In spite of the increase in total activity of the enzymes per liver cell, the plasmalogen content did not change appreciably upon proliferation (206).…”

Section: Localization and Topologymentioning

confidence: 95%

“…Peroxisome proliferation in liver by clofibrate or plasticizer results in a 2to 3-fold increase in total and specific activity of DHAP-AT (204)(205)(206) and a less than 2-fold increase in acyl/alkyl-DHAP reductase (205 , 207). However, in purified peroxisomes the specific activity of DHAP-AT exhibited no significant increase under conditions causing an at least 10-fold increase in fatty acid f3-oxidation specific activity (206).…”

Section: Localization and Topologymentioning

confidence: 99%

Biochemistry of Peroxisomes

Bosch

Schutgens²,

Wanders³

et al. 1992

Annu. Rev. Biochem.

796

233

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“…All experiments were carried out with a phospholipids mixture consisting of 3 volumes DOPE and 7 volumes DOPC. This composition was based on previous reports demonstrating that these lipids are the principal constituents, 28% phosphoethanolamine and 57% phosphocholine, respectively, of the membranes of rat liver peroxisomes (54). A mixture of both was spread on an aqueous subphase to form monolayers at the air/water interphase with an initial surface pressure of 20 mN/m.…”

Section: Membrane-associated Pex5p(w204a;w261a) Can Shuttle Back To Tmentioning

confidence: 99%

Membrane Association of the Cycling Peroxisome Import Receptor Pex5p

Kerssen

Hambruch

Klaas

et al. 2006

Journal of Biological Chemistry

106

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Peroxisomal proteins carrying a peroxisome targeting signal type 1 (PTS1) are recognized in the cytosol by the cycling import receptor Pex5p. The receptor-cargo complex docks at the peroxisomal membrane where it associates with multimeric protein complexes, referred to as the docking and RING finger complexes. Here we have identified regions within the Saccharomyces cerevisiae Pex5p sequence that interconnect the receptor-cargo complex with the docking complex. Site-directed mutagenesis of the conserved tryptophan residue within a reverse WXXXF motif abolished two-hybrid binding with the N-terminal half of Pex14p. In combination with an additional mutation introduced into the Pex13p-binding site, we generated a Pex5p mutant defective in a stable association not only with the docking complex but also with the RING finger peroxins at the membrane. Surprisingly, PTS1 proteins are still imported into peroxisomes in these mutant cells. Because these mutations had no significant effect on the membrane binding properties of Pex5p, we examined yeast and human Pex5p for intrinsic lipid binding activity. In vitro analyses demonstrated that both proteins have the potential to insert spontaneously into phospholipid membranes. Altogether, these data strongly suggest that a translocation-competent state of the PTS1 receptor enters the membrane via protein-lipid interactions before it tightly associates with other peroxins.

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Effect of peroxisome proliferation on ether phospholipid biosynthesizing enzymes in rat liver

Cited by 23 publications

References 34 publications

A PPARs cross‐talk concertedly commits C6 glioma cells to oligodendrocytes and induces enzymes involved in myelin synthesis

A PPARs cross‐talk concertedly commits C6 glioma cells to oligodendrocytes and induces enzymes involved in myelin synthesis

Biochemistry of Peroxisomes

Membrane Association of the Cycling Peroxisome Import Receptor Pex5p

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