Effect of perturbing diacylglycerol metabolism on cytosolic free Ca<sup>2+</sup> oscillations induced in single hepatocytes

Sanchez-Bueno, A; Cobbold, P H

doi:10.1016/0014-5793(92)81409-f

Cited by 5 publications

(1 citation statement)

References 16 publications

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“…[19]. Diacylglycerol is indeed a possible candidate for involvement since it is a natural activator of protein kinase C, which is known to have an inhibitory effect on the oscillatory Ca# + response of hepatocytes [20,21]. On the other hand, activation of protein kinase C, e.g.…”

Section: Figure 8 Simultaneous Measurements Of Ca 2 + In the Cytosol mentioning

confidence: 99%

Agonist-specific behaviour of the intracellular Ca2+ response in rat hepatocytes

Chatton

Cao

Stucki

1997

Biochemical Journal

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A variety of agonists stimulate in hepatocytes a response that takes the shape of repetitive cytosolic free Ca2+ transients called Ca2+ oscillations. The shape of spikes and the pattern of oscillations in a given cell differ depending on the agonist of the phosphoinositide pathway that is applied. In this study, the response of individual rat hepatocytes to maximal stimulation by arginine vasopressin (AVP), phenylephrine and ADP was investigated by fluorescence microscopy and flash photolysis. Hepatocytes loaded with Ca2+-sensitive probes were stimulated with a first agonist to evoke a maximal response, and then a second agonist was added. When phenylephrine or ADP was used as the first agonist, AVP applied subsequently could elicit an additional response, which did not happen when AVP was first applied and phenylephrine or ADP was applied later. Cells microinjected with caged myo-inositol 1,4,5-trisphosphate (IP3) were challenged with the different agonists and, when a maximal response was obtained, photorelease of IP3 was triggered. Cells maximally stimulated with AVP did not respond to IP3 photorelease, whereas those stimulated with phenylephrine or ADP responded with a fast Ca2+ spike above the elevated steady-state level, which was followed by an undershoot. In contrast, with all three agonists, IP3 photorelease triggered at the top of an oscillatory Ca2+ transient was able to mobilize additional Ca2+. These experiments indicate that the differential response of cells to agonists is found not only during Ca2+ oscillations but also during maximal agonist stimulation and that potency and efficacy differences exist among agonists.

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Section: Figure 8 Simultaneous Measurements Of Ca 2 + In the Cytosol mentioning

confidence: 99%

Agonist-specific behaviour of the intracellular Ca2+ response in rat hepatocytes

Chatton

Cao

Stucki

1997

Biochemical Journal

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Suppression of agonist induced Ca2+ oscillations in cultured hepatocytes by nafenopin: possible involvement of protein kinase C

1996

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The alpha 1-agonist phenylephrine (5 microM) induces an increase in the free cytosolic Ca2+ concentration, followed by repetitive transients of the cytoplasmic Ca2+ concentration, in single Fura-2 loaded hepatocytes. The tumor promoting, hypolipidemic drug nafenopin suppressed the cellular Ca2+ response to phenylephrine. The effect of nafenopin on the Ca2+ increase and Ca2+ oscillations was largely prevented by the specific protein kinase C inhibitor Gö 6976. This finding suggests involvement of protein kinase C in the action of nafenopin on phenylephrine induced Ca2+ mobilization.

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Both activators and inhibitors of protein kinase C promote the inhibition of phenylephrine-induced [Ca2+]i oscillations in single intact rat hepatocytes

Berrie¹,

Cobbold²

1995

Cell Calcium

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Effect of perturbing diacylglycerol metabolism on cytosolic free Ca²⁺ oscillations induced in single hepatocytes

Cited by 5 publications

References 16 publications

Agonist-specific behaviour of the intracellular Ca2+ response in rat hepatocytes

Agonist-specific behaviour of the intracellular Ca2+ response in rat hepatocytes

Suppression of agonist induced Ca2+ oscillations in cultured hepatocytes by nafenopin: possible involvement of protein kinase C

Both activators and inhibitors of protein kinase C promote the inhibition of phenylephrine-induced [Ca2+]i oscillations in single intact rat hepatocytes

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Effect of perturbing diacylglycerol metabolism on cytosolic free Ca2+ oscillations induced in single hepatocytes

Cited by 5 publications

References 16 publications

Agonist-specific behaviour of the intracellular Ca2+ response in rat hepatocytes

Agonist-specific behaviour of the intracellular Ca2+ response in rat hepatocytes

Suppression of agonist induced Ca2+ oscillations in cultured hepatocytes by nafenopin: possible involvement of protein kinase C

Both activators and inhibitors of protein kinase C promote the inhibition of phenylephrine-induced [Ca2+]i oscillations in single intact rat hepatocytes

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Effect of perturbing diacylglycerol metabolism on cytosolic free Ca²⁺ oscillations induced in single hepatocytes