Effect of Peucedanum japonicum Thunb on the Expression of Obesity-Related Genes in Mice on a High-Fat Diet

Nukitrangsan, Natthanan; Okabe, Takafumi; Toda, Takayoshi; Inafuku, Masashi; Iwasaki, Hiromichi; Yanagita, Teruyoshi; Oku, Hirosuke

doi:10.5650/jos.60.527

Cited by 17 publications

(15 citation statements)

References 31 publications

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“…The expression of Pcsk9, associated with hypercholesterolemia, decreased after berberine treatment of HFD-induced obese mice [54]. The gene Serpina12 was found to be downregulated by Peucedanum japonicum extract in HFD-induced obese mice [55]. These results suggest that PTE inhibits lipid accumulation not only by lipid transfer and oxidation mediated by PPARα regulation, but also by inhibition of genes participating in lipogenesis and cholesterol biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

Polygala tenuifolia extract inhibits lipid accumulation in 3T3-L1 adipocytes and high-fat diet–induced obese mouse model and affects hepatic transcriptome and gut microbiota profiles

Wang

Yen

Cheng

et al. 2017

Food & Nutrition Research

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Obesity, the excessive accumulation of lipids in the body, is closely associated with many prevalent human disorders. Continued efforts to identify plant extracts that exhibit anti-obesity effects have drawn much attention. This study investigated whether a Polygala tenuifolia extract (PTE) possesses anti-obesity activity and how PTE may affect liver gene expression and gut microbiota. We used 3T3-L1 adipocytes and a high-fat diet–induced obese mouse model to determine the effects of PTE on lipid accumulation. Next-generation sequencing analysis of liver gene expression and gut microbiota profiles following PTE treatment were conducted to elucidate possible mechanisms. We found that treatment of fully differentiated 3T3-L1 adipocytes with PTE inhibited lipid accumulation in the cells through reducing lipid formation and triglyceride content and by increasing lipase activity. No cytotoxicity was observed from the PTE treatment. After 5 weeks of treatment with PTE, the increased body weight, elevated serum triglyceride content, and liver steatosis in the high-fat diet–induced obese mice were each reduced. Liver transcriptomic analysis revealed that expression of genes involved in lipid and cholesterol metabolism was significantly altered. The low-grade chronic inflammation of obesity caused by a high-fat diet was also decreased after PTE treatment. In addition, treatment with PTE improved the relatively low Bacteroidetes/Firmicutes ratio in the gut of high-fat diet–fed mice through enrichment of the Proteobacteria population and reduction of the Deferribacteres population. In conclusion, treatment with PTE inhibited lipid accumulation by inducing the expression of the master transcription factor PPARα, attenuated the low-grade chronic inflammation of obesity, and also altered gut microbiota profiles. These results indicate that PTE has the potential to be developed into an anti-obesity food supplement and therapy. Abbreviations: Abcg5: ATP-binding cassette subfamily G member 5; ALT: alanine aminotransferase; AMPK: adenosine monophosphate-activated protein kinase; AST: aspartate aminotransferase; B/F: Bacteroidetes to Firmicutes [ratio]; C/EBPα: CCAAT/enhancer-binding protein alpha; CR: creatinine; Cyp51: cytochrome P450 family 51; DMEM: Dulbecco’s modified Eagle’s medium; Fabp5: fatty acid-binding protein 5; FBS: fetal bovine serum; Fdps: farnesyl diphosphate synthase; Glc: Glucose; HFD: high-fat diet; GO: gene ontology; HPRT: hypoxanthine guanine phosphoribosyl transferase; IBMS: 3-isobutyl-1-methylxanthine; Idi1: isopentenyl-diphosphate delta isomerase 1; IL-1β: interleukin-1-beta; Lpin1: phosphatidic acid phosphohydrolase; LPS: lipopolysaccharide; Mvd: mevalonate diphosphate decarboxylase; ND: normal diet; OTU: operational taxonomic units; Pcsk9: proprotein convertase subtilisin/kexin 9; Pctp: phosphatidylcholine transfer protein; PPARα: peroxisome proliferator-activated receptor alpha; PPARγ: peroxisome proliferator-activated receptor gamma; PTE: Polygala tenuifolia extract; Saa1: serum amyloid A1; SD: ...

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Section: Discussionmentioning

confidence: 99%

Polygala tenuifolia extract inhibits lipid accumulation in 3T3-L1 adipocytes and high-fat diet–induced obese mouse model and affects hepatic transcriptome and gut microbiota profiles

Wang

Yen

Cheng

et al. 2017

Food & Nutrition Research

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“…It localizes across a 5 Mb domain near its site of transcription and is in close proximity to five distinct transchromosomal loci, 47 four of which were previously described as having regulatory roles in adipogenesis. 79-82 RNA-pulldown assays showed that the heterogeneous nuclear ribonucleoprotein U (hnRNPU) binds to FIRRE and that the distinct 156 bp RNA repeats are necessary for this interaction. 47 Importantly, the FIRRE –hnRNPU interaction is needed for colocalization of the transchromosomal loci contacted by FIRRE , indicating that this lncRNA is an important nuclear organization factor that brings loci encoding known regulators of adipogenesis into close proximity within the nucleus and enables their co-regulation.…”

Section: Lncrnas In Endocrine Physiology and Diseasementioning

confidence: 99%

Long non-coding RNAs as regulators of the endocrine system

Knoll¹,

Lodish²,

Sun³

2015

Nat Rev Endocrinol

205

147

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Long non-coding RNAs (lncRNAs) are a large and diverse group of RNAs that are often lineage-specific and that regulate multiple biological functions. Many are nuclear and are essential parts of ribonucleoprotein complexes that modify chromatin segments and establish active or repressive chromatin states; others are cytosolic and regulate the stability of mRNA or act as microRNA sponges. This Review summarizes the current knowledge of lncRNAs as regulators of the endocrine system, with a focus on the identification and mode of action of several endocrine-important lncRNAs. We highlight lncRNAs that have a role in the development and function of pancreatic β cells, white and brown adipose tissue, and other endocrine organs, and discuss the involvement of these molecules in endocrine dysfunction (for example, diabetes mellitus). We also address the associations of lncRNAs with nuclear receptors involved in major hormonal signalling pathways, such as estrogen and androgen receptors, and the relevance of these associations in certain endocrine cancers.

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“…Our previous study using microarray and RT-PCR analyses revealed modulation of obesity-related gene expression in PJT-fed mice: RORC and PBEF1 were upregulated and DUSP1, INSIG2 and SERPINA12 were downregulated in the liver; FXRα and PPARγ were upregulated and PEG1/MEST, the size-marker of adipocytes, was downregulated in the adipose tissue 8 . The trends seen in the previous study were reproduced in the mice fed PJT ethanol extract in the present study, albeit with some inconsistency in the statistical significance.…”

Section: Discussionmentioning

confidence: 99%

“…It is, therefore, speculated that the fiber component of PJT is largely responsible for the anti-obesity activity of PJT. In previous studies, the increased dietary fiber intake derived from PJT was compensated by decreasing the cellulose content of the diet 7,8 . However, a compensatory decrease in dietary cellulose does not decisively address the question whether fi ber or other phytochemical components are responsible for the anti-obesity activity of PJT.…”

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confidence: 99%

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Effect of Peucedanum japonicum Thunb Extract on High-fat Diet-induced Obesity and Gene Expression in Mice

Nukitrangsan

Okabe

Toda³

et al. 2012

J. Oleo Sci.

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Effect of Peucedanum japonicum Thunb on the Expression of Obesity-Related Genes in Mice on a High-Fat Diet

Cited by 17 publications

References 31 publications

Polygala tenuifolia extract inhibits lipid accumulation in 3T3-L1 adipocytes and high-fat diet–induced obese mouse model and affects hepatic transcriptome and gut microbiota profiles

Polygala tenuifolia extract inhibits lipid accumulation in 3T3-L1 adipocytes and high-fat diet–induced obese mouse model and affects hepatic transcriptome and gut microbiota profiles

Long non-coding RNAs as regulators of the endocrine system

Effect of Peucedanum japonicum Thunb Extract on High-fat Diet-induced Obesity and Gene Expression in Mice

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