Effect of pH, agitation and aeration on hyaluronic acid production byStreptococcus zooepidemicus

Johns, Michael R.; Goh, Lin-Tang; Oeggerli, A.

doi:10.1007/bf01023334

Cited by 85 publications

(83 citation statements)

References 6 publications

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“…Interestingly, increased molecular weight in response to an empty plasmid was also observed during heterologous production of HA in Bacillus subtilis (44) suggesting that foreign DNA stress is beneficial for HA molecular weight. Indeed, there is evidence that stress in general (plasmid stress, aerobic conditions, changes in pH, and low temperature) is beneficial for high molecular weight HA production (16,17,20). Transcriptome, proteome, and metabolome analyses are underway to identify the molecular basis of this stress effect.…”

Section: Discussionmentioning

confidence: 99%

Hyaluronan Molecular Weight Is Controlled by UDP-N-acetylglucosamine Concentration in Streptococcus zooepidemicus

Chen

Marcellin

Hung

et al. 2009

Journal of Biological Chemistry

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The molecular weight of hyaluronan is important for its rheological and biological function. The molecular mechanisms underlying chain termination and hence molecular weight control remain poorly understood, not only for hyaluronan synthases but also for other ␤-polysaccharide synthases, e.g. cellulose, chitin, and 1,3-betaglucan synthases. In this work, we manipulated metabolite concentrations in the hyaluronan pathway by overexpressing the five genes of the hyaluronan synthesis operon in Streptococcus equi subsp. zooepidemicus. Overexpression of genes involved in UDP-glucuronic acid biosynthesis decreased molecular weight, whereas overexpression of genes involved in UDP-N-acetylglucosamine biosynthesis increased molecular weight. The highest molecular mass observed was at 3.4 ؎ 0.1 MDa twice that observed in the wild-type strain, 1.8 ؎ 0.1 MDa. The data indicate that (a) high molecular weight is achieved when an appropriate balance of UDP-N-acetylglucosamine and UDP-glucuronic acid is achieved, (b) UDP-N-acetylglucosamine exerts the dominant effect on molecular weight, and (c) the wild-type strain has suboptimal levels of UDP-Nacetylglucosamine. Consistent herewith molecular weight correlated strongly ( ‫؍‬ 0.84, p ‫؍‬ 3 ؋ 10 ؊5 ) with the concentration of UDP-N-acetylglucosamine. Data presented in this paper represent the first model for hyaluronan molecular weight control based on the concentration of activated sugar precursors. These results can be used to engineer strains producing high molecular weight hyaluronan and may provide insight into similar polymerization mechanisms in other polysaccharides.

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Section: Discussionmentioning

confidence: 99%

Hyaluronan Molecular Weight Is Controlled by UDP-N-acetylglucosamine Concentration in Streptococcus zooepidemicus

Chen

Marcellin

Hung

et al. 2009

Journal of Biological Chemistry

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“…Huang et al (2006) [9] found that under homogeneous agitation, aeration has no effect on cell growth but markedly enhances the HA yield with the threshold for HA synthesis set at the dissolved oxygen (DO) level of 5% air saturation. The above finding and other studies [3,5,10] 5 suggested that DO may plays a stimulant role for the cells to over-produce HA.…”

Section: Introductionmentioning

confidence: 58%

“…where HA is a capsular biopolymer shedding to the medium [4]. It has been known that the HA yield can be greatly enhanced under aerobic relative to anaerobic culture [3,5,6]. The culture conditions for streptococcal HA fermentation, such as temperature, pH, and medium composition have been well investigated by many groups and these studies have found several important features by implementing HA production by S.…”

Section: Introductionmentioning

confidence: 99%

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Proteomic investigation of the impact of oxygen on the protein profiles of hyaluronic acid‐producing Streptococcus zooepidemicus

Huang

Chen

et al. 2010

Proteomics Clinical Apps

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Abbreviations: HA, hyaluronic acid; 2-DE, two-dimensional gel electrophoresis; OD, optical density; DO, dissolved oxygen; IEF, isoelectric focusing; SDS-PAGE SDS-polyacrylamide gel electrophoresis; pI, isoelectric point; S. zooepidemicus, Streptococcus zooepidemicus; S. pyogenes, Streptococcus pyogenes; AoDHES, acetoin dehydrogenase enzyme system; glmU, UDP-N-acetyl-glucosamine pyrophosphorylase; NOX, NADH oxidase; acoA, acetoin dehydrogenase enzyme system E1 component α subunit; acoB, acetoin dehydrogenase enzyme system E1 component β subunit; acoC, dihydrolipoamide S-acetyltransferase; acoL,

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“…Previous investigators [5,6] have demonstrated that HA isolated from Streptococci has an average molecular weight in excess of 1 x 106. The inherent viscosity of HA which increases with increase in MW presents a major obstacle during fermentation as well as in purification [16]. Streptococcus species are already demonstrated in HA production.…”

Section: Fig 1: Ha Electrophoresis Separationmentioning

confidence: 99%

Hyaluronan-a Novel Polymer Isolated From Mutated Clinical Bacterial Isolate

Natarajan

Chandy

Sirajudeen

2018

Int J Pharm Pharm Sci

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Objective: This study was done to optimize the production parameters involved in the isolation of hyaluronan (HA) from UV mutated a clinical strain of Klebsiella pneumonia (M 3020).Methods: Glucose and nitrogen enriched media (D-glucose, L-glutamic acid, and peptone) were utilized to cultivate the clinical isolate K. pneumoniae. The strain was Ultra Violet (UV) radiation mutated (254 nm, 25 min) and HA production was optimized by parameters such as pH and temperature. The isolated HA from the fermented broth was subjected to purification by isopropyl alcohol and silica gel and further dried by lyophilization. Produced HA was confirmed with UV and Fourier Transform Infra-Red (FT-IR) spectroscopy.Results: UV treated strain at 254 nm for 25 min predominantly produce a high quantity of HA (3.5 g/l) in 37 °C, 300 rpm and pH 6.8 at 24 h run. UV and IR spectrum of produced HA showed strong similarity with the standard hyaluronan. Conclusion:To conclude, high quantity and quality of HA can be isolated from mutated clinical strains of K. pneumoniae.

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Effect of pH, agitation and aeration on hyaluronic acid production byStreptococcus zooepidemicus

Cited by 85 publications

References 6 publications

Hyaluronan Molecular Weight Is Controlled by UDP-N-acetylglucosamine Concentration in Streptococcus zooepidemicus

Hyaluronan Molecular Weight Is Controlled by UDP-N-acetylglucosamine Concentration in Streptococcus zooepidemicus

Proteomic investigation of the impact of oxygen on the protein profiles of hyaluronic acid‐producing Streptococcus zooepidemicus

Hyaluronan-a Novel Polymer Isolated From Mutated Clinical Bacterial Isolate

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