Effect of pH on Inhibition and Enhancement of Luminol–H2O2–Co2+ Chemiluminescence by Phenolic Compounds and Amino Acids

Cui, Hua; Shi, Ming‐Juan; Rong, Meng; Zhou, Jian; Lai, Chaohua; Lin, Xucong

doi:10.1562/be-03-28.1

Cited by 55 publications

(46 citation statements)

References 20 publications

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“…The activity of a chemical enhancer, which is the most attractive mode for increasing the sensitivity, is strongly dependant upon pH of the CL reaction system [41,42]. Therefore, the effect of pH on CL reaction of L-012 was investigated.…”

Section: Optimization Of CL Conditionsmentioning

confidence: 99%

An ultrasensitive and highly selective determination method for quinones by high-performance liquid chromatography with photochemically initiated luminol chemiluminescence

Ahmed

Kishikawa

Ohyama

et al. 2009

Journal of Chromatography A

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Quinones are a class of compounds of substantial toxicological and pharmacological interest. An ultrasensitive and highly selective chemiluminescence (CL) method was newly developed for the determination of quinones based on the utility of photochemically initiated luminol CL. The method involves ultraviolet (UV) irradiation of quinones to generate reactive oxygen species (ROS) through the unique photosensitization reaction accompanied with the photolytical generation of 3,6-dihydroxyphthalic acid (DHPA) from quinones. The photoproducts were detected by luminol CL reaction. Interestingly, it was noticed that DHPA had enhancement effect for the luminol CL. The generation of the enhancer (DHPA) in association with the oxidant (ROS) in the photochemical reaction greatly increases the sensitivity and selectivity of the proposed luminol CL method. In order to elucidate the type of ROS produced by the photosensitizaion reaction in relation to the proposed CL reaction, we investigated the quenching effect of selective ROS scavengers in the luminol CL. Although several ROS were generated, superoxide anion was the most effective ROS for the generated CL. Moreover, the enhancement mechanism of DHPA for luminol CL was confirmed. The enhancement was found to be through the formation of stabilized semiquinone anion radical that provided long-lived CL. The generation of the semiquinone radical was confirmed by electron spin resonance technique. Furthermore, we developed an HPLC method with on-line photochemical reaction followed by the proposed CL detection for the determination of four quinones. A luminol analogue, L-012, was used for its high sensitivity. The detection limits for quinones obtained with the proposed method (S/N=3) were in the range 1.5-24 fmol that were 10-1000 times more sensitive compared with the previous methods. Finally, the developed HPLC-CL system was successfully applied for the determination of quinones in airborne particulate samples collected at Nagasaki city.

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Section: Optimization Of CL Conditionsmentioning

confidence: 99%

An ultrasensitive and highly selective determination method for quinones by high-performance liquid chromatography with photochemically initiated luminol chemiluminescence

Ahmed

Kishikawa

Ohyama

et al. 2009

Journal of Chromatography A

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“…In fact, it has been reported that some compounds either inhibit or enhance the luminol CL, depending on the pH of the solution. 23 With an increase in pH, inhibition for most compounds tended to convert toward enhancement. Furthermore, in the SIA methodology luminol was dissolved in dimethylformamide and sodium azide was dissolved in dimethylsulfoxide.…”

Section: Evaluation Of the Scavenging Capacity Against 1 O2mentioning

confidence: 99%

Automatic Multi-pumping Flow System for the Chemiluminometric Screening of Scavenging Capacity against Singlet Oxygen

et al. 2011

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“…33,36,37 In this way, the quenching of CL by SIN was perhaps due to competition for superoxide anion between luminol and SIN, in which a hydroxyl was included.…”

Section: Analytical Applicationsmentioning

confidence: 99%

Trace Analysis of Sinomenine Hydrochloride Using CdTe/CdS Quantum Dots-enhanced Chemiluminescence

Han

2015

ANAL. SCI.

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A novel flow-injection chemiluminescence (FI-CL) method was described for the determination of sinomenine hydrochloride (SIN). The method was based on the inhibitory effect of SIN on the CL reaction of luminol and K3Fe(CN)6 in an alkaline solution, which was sensitized by CdTe/CdS quantum dots (QDs). Under the optimized conditions, the linear range for the determination of SIN was 1.0 × 10 -8 to 1.4 × 10 -6 mol/L. The detection limit was 7.5 × 10 -9 mol/L, and the relative standard deviation was 2.47% (n = 11). The current CL method was applied to determine SIN in pharmaceutical formulations and biological fluids with satisfactory results. The possible CL reaction mechanism was discussed briefly.

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Effect of pH on Inhibition and Enhancement of Luminol–H2O2–Co2+ Chemiluminescence by Phenolic Compounds and Amino Acids

Cited by 55 publications

References 20 publications

An ultrasensitive and highly selective determination method for quinones by high-performance liquid chromatography with photochemically initiated luminol chemiluminescence

An ultrasensitive and highly selective determination method for quinones by high-performance liquid chromatography with photochemically initiated luminol chemiluminescence

Automatic Multi-pumping Flow System for the Chemiluminometric Screening of Scavenging Capacity against Singlet Oxygen

Trace Analysis of Sinomenine Hydrochloride Using CdTe/CdS Quantum Dots-enhanced Chemiluminescence

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