Effect of pH on thrombin activity measured by calibrated automated thrombinography

Jackson, Joseph W.; Surov, Stepan S.; Liang, Yideng; Parunov, Leonid A.; Ovanesov, Mikhail V.

doi:10.1002/rth2.12370

Cited by 3 publications

(3 citation statements)

References 3 publications

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“…It is an interesting figure (Figure 1 in Jackson et al2) showing the effect of pH on thrombin activity toward the fluorogenic substrate, which is very distinct—data that we did not know. However, we do not think that it is possible to overcome the effect of a high pH in the plasma sample using a calibrator with a matching pH.…”

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confidence: 77%

“…In the assay, we mix 80 µL of plasma with either 20 µL of trigger reagent or 20 µL of calibrator reagent; that is, in both instances plasma with a high pH is added, and consequently the wells with trigger and calibrator are pH matched (we expect that both of these reagents contain 20 mM of HEPES). We imagine that the activity of several coagulation factors is affected by the abnormal pH as also stated by Jackson et al, 2 and this will reduce the measured TG.…”

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confidence: 80%

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Reply to a letter from Jackson J et al: Effect of pH on thrombin activity measured by calibrated automated thrombinography

Kristensen

Nybo

Pedersen

2020

Research and Practice in Thrombosis and Haemostasis

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We wish to thank Joseph Jackson and coworkers for their interest in our paper and for elaborating and commenting on effects of pH on thrombin generation (TG). We agree that it is possible to choose other buffers, and lactic acid has been used for investigation of thrombin-induced fibrin formation. 1 An advantage is that it is a physiological component in blood, although using the highest suggested concentra-

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confidence: 77%

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confidence: 80%

Reply to a letter from Jackson J et al: Effect of pH on thrombin activity measured by calibrated automated thrombinography

Kristensen

Nybo

Pedersen

2020

Research and Practice in Thrombosis and Haemostasis

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“…The anticoagulant effect is believed to, at least partly, be a result of residual lactic acid from the precursor, possibly causing lactic acidosis [ 42 ]. A recent publication has shown a very clear effect of pH on thrombin activity, where a decrease in one pH unit from 7.5 to 6.5 resulted in a reduced thrombin activity of about 65% [ 43 ]. Corresponding extrapolation down to pH 6, which was the actual pH of the CNF_PEG_TiBALDH surface, would result in a thrombin activity of less than 10%, which explains the inhibited thrombin generation as well as the subsequent TAT and clot formation.…”

Section: Discussionmentioning

confidence: 99%

Hemocompatibility of Nanotitania-Nanocellulose Hybrid Materials

et al. 2021

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In order to develop a new type of improved wound dressing, we combined the wound healing properties of nanotitania with the advantageous dressing properties of nanocellulose to create three different hybrid materials. The hemocompatibility of the synthesized hybrid materials was evaluated in an in vitro human whole blood model. To our knowledge, this is the first study of the molecular interaction between hybrid nanotitania and blood proteins. Two of the hybrid materials prepared with 3 nm colloidal titania and 10 nm hydrothermally synthesized titania induced strong coagulation and platelet activation but negligible complement activation. Hence, they have great potential as a new dressing for promoting wound healing. Unlike the other two, the third hybrid material using molecular ammonium oxo-lactato titanate as a titania source inhibited platelet consumption, TAT generation, and complement activation, apparently via lowered pH at the surface interface. It is therefore suitable for applications where a passivating surface is desired, such as drug delivery systems and extracorporeal circuits. This opens the possibility for a tailored blood response through the surface functionalization of titania.

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Thrombin Generation in Trauma Patients: How Do we Navigate Through Scylla and Charybdis?

Mitrophanov

Vandyck

Tanaka

2022

Curr Anesthesiol Rep

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Purpose of Review Coagulopathy is a complex pathological condition manifested through the insufficient or excessive tendency to form blood thrombi, which can be caused by trauma and surgery. A promising strategy to mitigate coagulopathy is the balanced restoration of the generation of thrombin — the main enzyme in the blood-coagulation system. We review studies focusing on thrombin, traumatic coagulopathy, and hemostatic balance — a concept aimed to avoid abnormally weak or excessively strong coagulation responses. Recent Findings Thrombin generation is impacted by coagulopathic conditions described by complex patterns, possibly depending on various factors. New methodologies of thrombin-generation measurement and analysis are emerging. Combinations of pro- and anticoagulant proteins can restore thrombin generation in coagulopathy. Balanced therapeutic strategies to improve thrombin generation may lead to favorable clinical outcomes. Summary Thrombin generation is an essential target of pharmacological intervention in traumatic and surgical coagulopathy. Graphic Abstract

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Effect of pH on thrombin activity measured by calibrated automated thrombinography

Cited by 3 publications

References 3 publications

Reply to a letter from Jackson J et al: Effect of pH on thrombin activity measured by calibrated automated thrombinography

Reply to a letter from Jackson J et al: Effect of pH on thrombin activity measured by calibrated automated thrombinography

Hemocompatibility of Nanotitania-Nanocellulose Hybrid Materials

Thrombin Generation in Trauma Patients: How Do we Navigate Through Scylla and Charybdis?

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