Effect of pH, pCO2 and bicarbonate on metabolism of glucose by perfused rat heart

Delcher, Harry K.; Shipp, Joseph C.

doi:10.1016/0304-4165(66)90114-0

Cited by 47 publications

(13 citation statements)

References 31 publications

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“…This effect has previously been observed and has been explained by a lactate production by the muscles and other tissues due to stimulation of the glycolysis (Delcher and Shipp, 1966) when the blood is alkalized. Different groups have shown that b-hydroxybutyrate increases the production of lactate by the brain due to an inhibition of the pyruvate dehydrogenase enzyme.…”

Section: Alterations In Cerebral Blood Flowmentioning

confidence: 86%

Global Cerebral Blood Flow and Metabolism during Acute Hyperketonemia in the Awake and Anesthetized Rat

Linde

Hasselbalch

Topp

et al. 2006

J Cereb Blood Flow Metab

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In the human setting, it has been shown that acute increase in the concentration of ketone bodies by infusion of b-hydroxybutyrate increased the cerebral blood flow (CBF) without affecting the overall cerebral metabolic activity. The mechanism by which this effect of ketone bodies was mediated is not known. Alterations in several parameters may possibly explain the increase in CBF and the resetting of the relation between CBF and cerebral metabolism. To study this phenomenon further, we measured global CBF and global cerebral metabolism with the Kety-Schmidt technique in the wakeful rat before and during infusion of ketone bodies. During acute hyperketonemia (average concentration of b-hydroxybutyrate: 6 mmol/L), global CBF increased 65% from 108 to 178 mL/ 100 g min and the cerebral metabolic rates for both oxygen and glucose remained constant. This resetting of the relation between CBF and cerebral metabolism could not be explained by alterations in blood pH or arterial CO 2 tension. By measuring cerebral intracellular pH by 31 P nuclear magnetic resonance spectroscopy, it could further be concluded that the brain pH was unchanged during acute hyperketonemia. These observations indicate that the mechanism responsible for the increase in CBF is rather a direct effect on the cerebral endothelium than via some metabolic interactions.

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Section: Alterations In Cerebral Blood Flowmentioning

confidence: 86%

Global Cerebral Blood Flow and Metabolism during Acute Hyperketonemia in the Awake and Anesthetized Rat

Linde

Hasselbalch

Topp

et al. 2006

J Cereb Blood Flow Metab

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“…Previous work has demonstrated that alkalosis increases glycolysis in the isolated perfused rat heart [22,11,28]. Analysis of myo cardial hexose-and triosc-phosphale compounds suggests that the release of phosphofructokina.se inhibition is an important factor in this glycolytic stimulation [28].…”

mentioning

confidence: 99%

The Effects of Respiratory and Metabolic Alkalosis on Coronary Flow, Hemodynamics and Myocardial Carbohydrate Metabolism

Scheuer¹

1968

Cardiology

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“…Rates of glucose consumption were found to increase as a linear function of pH and therefore it was possible to quantify the pH effect. A linear response of the glycolytic rate to changes in pH is evident in the data of Delcher & Schipp (1966), who studied this effect in perfused rat hearts. In contrast with our findings, Ibsen et al (1960) found that Ehrlich ascites cells obtained from animals increased glucose consumption rates as pH was increased from pH6.3 to 7.2, but not on further increases to pH7.6 (effects of pH above 7.6 were not tested).…”

Section: Discussionmentioning

confidence: 95%

“…After the suggestions by Delcher & Schipp (1966) and by Ui (1966), the effect of pH on glycolysis has been thought to be due to its effect on phosphofructokinase and other glycolytic enzymes. Halperin et al (1969) and Wilhelm et al (1971) concluded that phosphofructokinase, glyceraldehyde 3-phosphate dehydrogenase or phosphoglycerate kinase, and pyruvate kinase were the main control points in pHdependence of glycolysis.…”

Section: Discussionmentioning

confidence: 99%

“…The pH-induced changes in glycolytic rates are large, and may constitute a major cellular defence mechanism against alkalosis and acidosis. The effect of pH has been generally ascribed to changes in activities of several key glycolytic enzymes, in particular phosphofructokinase (Delcher & Schipp, 1966;Ui, 1966;Halperin et al, 1969;Wilhelm et al, 1971;Fodge & Rubin, 1973).…”

mentioning

confidence: 99%

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The pH-dependence of sugar-transport and glycolysis in cultured Ehrlich ascites-tumour cells

Kaminskas¹

1978

Biochemical Journal

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1. pH-dependence of glycolysis has generally been ascribed to the effects of pH on the activities of glycolytic enzymes. The present study shows that sugar transport is pHdependent in cultured Ehrlich ascites-tumour cells. 2. The rates of glucose consumption, of 3-0-methylglucose transport, and of 2-deoxyglucose transport and phosphorylation increased as linear functions of pH, as the pH ofthe cell culture medium was increased from 6.1 to 8.5. Transport of glucose, as measured in ATP-depleted cells, was pH-dependent to the same extent as transport of the non-metabolizable sugars. 3. Glucose consumption rates were about 8-fold higher at pH 8.5 than at pH 6.4. About 65-85% of glucose was converted into lactate. Sugar transport rates were 2.5-fold higher at pH 8.5 than at pH 6.3. 4. pH affected both simple diffusion and facilitated diffusion. pH effect was mainly on the Vmax. of 2-deoxyglucose uptake, and on the rapid-uptake phase of 3-0-methylglucose transport. 5. It was estimated that about 70% of the pH effect on the rates of glucose consumption may be due to the effect on sugar transport and the remainder to the effect on the activities of glycolytic enzymes.

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Effect of pH, pCO2 and bicarbonate on metabolism of glucose by perfused rat heart

Cited by 47 publications

References 31 publications

Global Cerebral Blood Flow and Metabolism during Acute Hyperketonemia in the Awake and Anesthetized Rat

Global Cerebral Blood Flow and Metabolism during Acute Hyperketonemia in the Awake and Anesthetized Rat

The Effects of Respiratory and Metabolic Alkalosis on Coronary Flow, Hemodynamics and Myocardial Carbohydrate Metabolism

The pH-dependence of sugar-transport and glycolysis in cultured Ehrlich ascites-tumour cells

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