Effect of pharynx epithelial cells surface desialylation on receptor-mediated adherence of<i>Staphylococcus aureus</i>

Sakarya, Serhan; Ertuğrul, Mustafa Bülent; Ozturk, Turgut; Gökbulut, Cengiz

doi:10.1111/j.1365-2672.2009.04525.x

Cited by 10 publications

(7 citation statements)

References 53 publications

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“…In contrast to other staphylococcal genome sequences, S. pseudintermedius ED99 and S. delphini 8086 genomes both contain genes encoding a putative sialidase or neuraminidase. The neuraminidase modification of host sugars may contribute to host colonization by providing a carbon source for growth, contributing to biofilm formation, or by enhancing adherence by exposing receptors on the host cell (Sakarya et al, 2010). Overall, the SIG species share a large number of toxins and exoenzymes reflecting their recent common ancestry and possibly their common skin niche.…”

Section: Resultsmentioning

confidence: 99%

Comparative Genomics of the Staphylococcus intermedius Group of Animal Pathogens

Zakour¹,

Beatson²,

Broek³

et al. 2012

Front. Cell. Inf. Microbio.

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The Staphylococcus intermedius group consists of three closely related coagulase-positive bacterial species including S. intermedius, Staphylococcus pseudintermedius, and Staphylococcus delphini. S. pseudintermedius is a major skin pathogen of dogs, which occasionally causes severe zoonotic infections of humans. S. delphini has been isolated from an array of different animals including horses, mink, and pigeons, whereas S. intermedius has been isolated only from pigeons to date. Here we provide a detailed analysis of the S. pseudintermedius whole genome sequence in comparison to high quality draft S. intermedius and S. delphini genomes, and to other sequenced staphylococcal species. The core genome of the SIG was highly conserved with average nucleotide identity (ANI) between the three species of 93.61%, which is very close to the threshold of species delineation (95% ANI), highlighting the close-relatedness of the SIG species. However, considerable variation was identified in the content of mobile genetic elements, cell wall-associated proteins, and iron and sugar transporters, reflecting the distinct ecological niches inhabited. Of note, S. pseudintermedius ED99 contained a clustered regularly interspaced short palindromic repeat locus of the Nmeni subtype and S. intermedius contained both Nmeni and Mtube subtypes. In contrast to S. intermedius and S. delphini and most other staphylococci examined to date, S. pseudintermedius contained at least nine predicted reverse transcriptase Group II introns. Furthermore, S. pseudintermedius ED99 encoded several transposons which were largely responsible for its multi-resistant phenotype. Overall, the study highlights extensive differences in accessory genome content between closely related staphylococcal species inhabiting distinct host niches, providing new avenues for research into pathogenesis and bacterial host-adaptation.

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Section: Resultsmentioning

confidence: 99%

Comparative Genomics of the Staphylococcus intermedius Group of Animal Pathogens

Zakour¹,

Beatson²,

Broek³

et al. 2012

Front. Cell. Inf. Microbio.

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“…The nanB gene for sialidase may contribute to host colonization by providing a carbon source for growth, contributing to biofilm formation, or by enhancing adherence by exposing receptors on the host cell (69). Sialidase enzymes help regulate sialic acid on cell surfaces, which are important determinants of eukaryotic cell-cell interactions (71). Perhaps this virulence gene has played a role in the success of this ST.…”

Section: Discussionmentioning

confidence: 99%

Molecular Epidemiology of Clinical and Colonizing Methicillin-Resistant Staphylococcus Isolates in Companion Animals

et al. 2021

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In this study, we aimed to investigate the molecular epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP) clinical and colonizing isolates of dogs and cats to profile contributing factors associated with their isolation. Nasal and rectal samples were collected from dogs and cats between 2015 and 2017 to identify colonizing isolates. Clinical isolates collected between 2003 and 2016 were retrieved from a Queensland university veterinary diagnostic laboratory. All isolates were identified using standard microbiological and molecular methods and were characterized by whole genome sequencing. Phylogenetic relationships and differences in epidemiological factors were investigated. Seventy-two MRSP isolates out of 1,460 colonizing samples and nine MRSP clinical isolates were identified. No MRSA was isolated. ST496 and ST749 were the most commonly isolated sequence types with different SCCmec types. ST496 clones spread both along the coast and more inland where ST749 was more centered in Brisbane. The resistance and virulence factors differed significantly between the two sequence types. ST496 colonizing and clinical isolates were similarly multidrug resistant. The virulence genes of ST749 colonizing and clinical isolates were similar as both contained the gene nanB for sialidase. There were no differences in the individual and clinical factors between predominant sequence types. High levels of antimicrobial resistance occurred in the majority of isolates, which is of potential concern to human and veterinary health. The phylogenetic clustering of isolates from this study and others previously identified in countries, particularly New Zealand, with which Australia has high volume of pet movements could suggest the importation of clones, which needs further investigation.

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“…The fluctuating glucose levels occurring in the nasal cavity implies that S. aureus has to resort to other carbon sources, which led us to examine how S. aureus sustains NO stress when utilizing other hexoses available in the nasal niche (King, 2010; Krismer et al, 2014; Blanchette et al, 2016). In particular, galactose is important for the colonization of S. aureus , as studies have shown that: (i) S. aureus binds human nasal galactose rich mucins during colonization (Shuter et al, 1996); (ii) S. aureus binds galactose molecules in host glycoproteins (Sakarya et al, 2010); (iii) the S. aureus tagatose 6-phosphate pathway is essential for the catabolism of lactose/galactose, which are sugars present in milk and derivative products (Miallau et al, 2007); and (iv) the malfunctioning of the S. aureus tagatose 6-phosphate pathway leads to accumulation of the dietary D -galactose causing bacterial cell death (Miallau et al, 2007). While in many bacteria, galactose is predominantly metabolized through the Leloir pathway, in S. aureus the galactose breakdown occurs through the tagatose 6-phosphate pathway.…”

Section: Introductionmentioning

confidence: 99%

The Staphylococcus aureus α-Acetolactate Synthase ALS Confers Resistance to Nitrosative Stress

et al. 2017

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Staphylococcus aureus is a worldwide pathogen that colonizes the human nasal cavity and is a major cause of respiratory and cutaneous infections. In the nasal cavity, S. aureus thrives with high concentrations of nitric oxide (NO) produced by the innate immune effectors and has available for growth slow-metabolizing free hexoses, such as galactose. Here, we have used deep sequencing transcriptomic analysis (RNA-Seq) and 1H-NMR to uncover how S. aureus grown on galactose, a major carbon source present in the nasopharynx, survives the deleterious action of NO. We observed that, like on glucose, S. aureus withstands high concentrations of NO when using galactose. Data indicate that this resistance is, most likely, achieved through a distinct metabolism that relies on the increased production of amino acids, such as glutamate, threonine, and branched-chain amino acids (BCAAs). Moreover, we found that under NO stress the S. aureus α-acetolactate synthase (ALS) enzyme, which converts pyruvate into α-acetolactate, plays an important role. ALS is proposed to prevent intracellular acidification, to promote the production of BCAAs and the activation of the TCA cycle. Additionally, ALS is shown to contribute to the successful infection of murine macrophages. Furthermore, ALS contributes to the resistance of S. aureus to beta-lactam antibiotics such as methicillin and oxacillin.

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Effect of pharynx epithelial cells surface desialylation on receptor-mediated adherence ofStaphylococcus aureus

Cited by 10 publications

References 53 publications

Comparative Genomics of the Staphylococcus intermedius Group of Animal Pathogens

Comparative Genomics of the Staphylococcus intermedius Group of Animal Pathogens

Molecular Epidemiology of Clinical and Colonizing Methicillin-Resistant Staphylococcus Isolates in Companion Animals

The Staphylococcus aureus α-Acetolactate Synthase ALS Confers Resistance to Nitrosative Stress

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