Effect of phenobarbital on bile flow and bile salt excretion in the rat

Paumgartner, Gustav; Horak, W; Probst, Peter; Grabner, G

doi:10.1007/bf00997305

Cited by 124 publications

(40 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The bile flow rate was determined gravimetrically assuming the specific gravity of bile to be 1.0. Total bile salt concentration in the bile was deter-mined enzymatically as previously reported (PAUMGARTNER et al, 1971). The individual bile salt fractions, ursodeoxycholate, and its taurine and glycine conjugates in the bile were separated by thin layer chromatography and further subjected to enzymatic analysis as previously described (KITANI and KANAI, 1982).…”

mentioning

confidence: 99%

Ursodeoxycholate-induced choleresis in taurine-deprived and taurine-supplemented rats.

Kitani

Kanai

1985

Jpn.J.Physiol

View full text Add to dashboard Cite

The effect of i.v. infused ursodeoxycholate (1.2 ,umol/(min 100 g body weight)) was compared in control rats and in taurine supplemented (i.p. administration of a taurine solution) and taurine deprived (oral administration of 13-alanine) rats. In the control rats, the bile water output during the first hr was significantly lower than that of the second hr, while the bile salt output was significantly lower in the second hr. In j3-alanine pretreated rats, the increase in the bile flow in the first hr was more rapid compared to control rats, and the bile flow rate reached a peak earlier. The first hr and total 2 hr bile water outputs were significantly higher in the j3-alanine pretreated rats compared to the corresponding bile flow values of the control rats, while the bile salt outputs were significantly lower than respective control values. In taurine supplemented rats, the increase in bile flow in the second hr was less than in control rats, while the bile salt excretion rate was the highest among the three rat groups. Thus, among these three groups, the bile flow and bile salt excretion rate during the first hr and 2 hr total infusion periods were negatively correlated. On the other hand, a significant positive correlation was observed between the bile flow rate for 1 or 2 hr and bile salt concentration in the liver examined after a 1 or 2 hr bile salt infusion. These results seem most easily explained by our previous thesis that at least part of the bile is produced not by the excretion of bile salt into the bile canaliculus but by its presence inside the hepatocyte (secretory mechanism).

show abstract

mentioning

confidence: 99%

Ursodeoxycholate-induced choleresis in taurine-deprived and taurine-supplemented rats.

Kitani

Kanai

1985

Jpn.J.Physiol

View full text Add to dashboard Cite

show abstract

“…Total bile acid concentration in bile was deter mined enzymatically [6]. Cholesterol was measured by the Liebermann-Burchard reaction after extraction with petroleum ether [7], Phospholipids were ex tracted from bile by chloroform and determined with Fiske-Subbarow reagent according to the method of Bartlett [8].…”

Section: Methodsmentioning

confidence: 99%

Bile Acid Pattern and Cholesterol Saturation of Bile after Cholecystectomy and Endoscopic Sphincterotomy

Stellaard¹,

Sauerbruch²,

Brunhölzl³

et al. 1983

Digestion

Self Cite

View full text Add to dashboard Cite

The effect of endoscopic sphincterotomy on bile acid composition and cholesterol saturation of bile has been studied in cholecystectomized patients. Individual bile acids and biliary lipids were measured in hepatic bile of 13 cholecystectomized females aged 56.8 ± 16.6 years more than 9 months (mean 16.7 ± 8.8 months) after sphincterotomy and of 12 cholecystectomized females aged 59.3 ± 11.5 years who served as controls. The sphincterotomy group exhibited a significantly (p < 0.01) higher percentage of chenodeoxycholic acid in bile – 39.2 ± (SD) 7.7% – than the controls with cholecystectomy only (29.1 ± 7.4%), but showed no differences in the proportion of cholic acid (32.4 ± 6.2 vs. 33.6 ± 7.8%). The percentages of the secondary bile acids, deoxycholic acid (25.0 ± 8.8 vs. 32.3 ± 8.3%), and lithocholic acid (1.7 ± 0.8 vs. 2.6 ± 2.3%) were lower, but these differences were not statistically significant. The biliary lipid composition in the sphincterotomy group was not different from that in the controls, resulting in a similar cholesterol saturation index in both groups (1.87 ± 0.60 vs. 2.02 ± 0.60 according to Carey and Small; 1.45 ± 0.32 vs. 1.55 ± 0.32 according to Hegardt and Dam). These findings do not demonstrate any alterations of the bile composition after sphincterotomy which may be expected to have undesirable effects on the biliary and/or gastrointestinal system.

show abstract

“…Bile acid concentrations in bile were determined enzymically by the method ofTalalay (1960) as modified by Paumgartner et al (1971). Cholesterol concentrations in bile were determined by a modification of the method of Bolton et al (1980) using a commercial kit (Cholesterol enzymatique PAP; Biomerieux, Charboniers les Bains, France).…”

Section: Specific Determinationsmentioning

confidence: 99%

Inhibition of biliary cholesterol and phospholipid secretion by cefmetazole. The role of vesicular transport and of canalicular events

Cava

González

González-Buitrago

et al. 1991

Biochemical Journal

View full text Add to dashboard Cite

A number of organic anions selectively inhibit the biliary secretion of cholesterol and phospholipids without affecting bile acid secretion. We studied the effect of cefmetazole, a third-generation cephalosporin, on biliary lipid secretion in the rat. Injection of cefmetazole at a dose of 200,mol/kg body wt. induced a choleretic effect and a significant decrease in the biliary output of cholesterol and phospholipid, without changes in bile acid secretion. The decrease was more marked for cholesterol than for phospholipid secretion, with a significant decrease in their molar ratio in bile. The effects were apparently unrelated to an inhibition of intracellular vesicular transport because, after injection of horseradish peroxidase, both the time course and total amount secreted of the protein did not significantly differ between control animals and those receiving cefmetazole. The secretory rate of the lysosomal marker acid phosphatase was not affected by cefmetazole administration. Biliary outputs of the plasma-membrane enzymes alkaline phosphatase and y-glutamyltransferase were significantly decreased by the antibiotic. These results point to an effect of cefmetazole at the level of the canalicular membrane.

show abstract

Effect of phenobarbital on bile flow and bile salt excretion in the rat

Cited by 124 publications

References 5 publications

Ursodeoxycholate-induced choleresis in taurine-deprived and taurine-supplemented rats.

Ursodeoxycholate-induced choleresis in taurine-deprived and taurine-supplemented rats.

Bile Acid Pattern and Cholesterol Saturation of Bile after Cholecystectomy and Endoscopic Sphincterotomy

Inhibition of biliary cholesterol and phospholipid secretion by cefmetazole. The role of vesicular transport and of canalicular events

Contact Info

Product

Resources

About