Effect of Phenolic Extract from Red Beans (Phaseolus vulgaris L.) on T-2 Toxin-Induced Cytotoxicity in HepG2 Cells

Abstract

Red beans contain human bioactive compounds such as polyphenols. Several in vitro studies have proposed the natural compounds as an innovative strategy to modify the toxic effects produced by mycotoxins. Hence, in this work, a complete investigation of the polyphenolic fraction of red beans was performed using a Q-Orbitrap high-resolution mass spectrometry analysis. Notably, epicatechin and delphinidin were the most detected polyphenols found in red bean extracts (3.297 and 3.108 mg/Kg, respectively). Moreover, the red bean extract was evaluated against the T-2 toxin (T-2) induced cytotoxicity in hepatocarcinoma cells (HepG2) by direct treatment, simultaneous treatment, and pre-treatment assays. These data showed that T-2 affected the cell viability in a dose-dependent manner, as well as observing a cytotoxic effect and a significant increase in ROS production at 30 nM. The simultaneous treatment and the pre-treatment of HepG2 cells with red bean extract was not able to modify the cytotoxic T-2 effect. However, the simultaneous treatment of T-2 at 7.5 nM with the red bean extract showed a significant decrease in ROS production, with respect to the control. These results suggest that the red bean extract could modulate oxidative stress on HepG2 cells.

Evaluation of the Bioaccessible Fraction of T-2 Toxin from Cereals and Its Effect on the Viability of Caco-2 Cells Exposed to Tyrosol

“…Similarly, 15 and 30 nM T-2 significantly decreased ( p ≤ 0.05) the cell viability, while cells exposed to 7.5 nM T-2 did not show significant differences in cell viability with respect to the control. On the other hand, Martínez-Alonso et al [ 27 ] studied the viability of human hepatocarcinoma HepG2 cells exposed to T-2 (7.5, 15 and 30 nM) and observed a cytotoxic effect with 30 nM T-2. However, these authors reported that 15 nM T-2 did not affect cell viability, and 7.5 nM T-2 significantly increased cell viability due to a possible hormetic effect in HepG2 cells.…”

Effect of Red Bean Extract (Phaseolus Vulgaris L. Sp) on Il-6 Levels and AMH Levels as The Prevention of Premature Ovarian Failure in Mice Model of The Systemic Lupus Erythematosus

“…Phenolic compounds, besides from contributing to the smell, taste, and color of food, have a long-term intake that could play a bioactive role due to their antioxidant activity which has been related to the prevention of obesity, cardiovascular and neurodegenerative diseases, cancer, and diabetes, as well as exhibiting anti-inflammatory, antimutagenic, and antibacterial properties [11]. Madhujith et al demonstrated that beans, especially those with colored skins, possess strong antioxidant activity as measured by different model systems [12].…”

In Vitro Cytotoxic Effect of Aqueous Extracts from Leaves and Rhizomes of the Seagrass Posidonia oceanica (L.) Delile on HepG2 Liver Cancer Cells: Focus on Autophagy and Apoptosis

“…Delphinidin-3-glucoside and quercetin 3-O-galactoside were the most abundant polyphenols in RE and lower amounts of vanillic acid and some procyanidin dimers were also above the quantitation-allowing threshold. Interestingly, Maciel et al [ 68 ] reported that a low amount (5.39 μg/mL) of the delphinidin-3-glucoside-rich aqueous extract from Hibiscus sabdariffa acted as LC 50 (50% lethal concentration) in HepG2 cells, whereas Martìnez-Alonso et al [ 69 ] showed that the delphinidin-rich red bean extract acted by reducing ROS production. In addition, quercetin 3-O-galactoside (also known as hyperoside) was reported to arrest the HepG2 cell cycle by down-regulating the BMP-7/PI3K/AKT signaling [ 70 ].…”