1988
DOI: 10.1016/0167-4889(88)90215-7
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Effect of phorbol myristate acetate and concanavalin A on the glycolytic enzymes of human peripheral lymphocytes

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Cited by 21 publications
(27 citation statements)
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“…Consistent with this observation, 3 H-thymidine incorporation into DNA increases with increasing glucose concentration in mitogen stimulated rat thymocytes (Hume et al 1978). In stimulated human peripheral lymphocytes, glycolytic enzyme activities, lactate production, and DNA synthesis reach maximum levels in S phase of the cell cycle (Marjanovic et al 1988). Further, studies of cell proliferation in the absence of glucose have demonstrated that HeLa cells, mouse L cells, early passage human fibroblasts, and primary chicken embryo fibroblasts can all grow indefinitely at near maximal growth rates without glucose as long as they are supplemented with glutamine and uridine (Wice et al 1981).…”
Section: A Major Function Of Aerobic Glycolysis Is To Support Macromosupporting
confidence: 64%
“…Consistent with this observation, 3 H-thymidine incorporation into DNA increases with increasing glucose concentration in mitogen stimulated rat thymocytes (Hume et al 1978). In stimulated human peripheral lymphocytes, glycolytic enzyme activities, lactate production, and DNA synthesis reach maximum levels in S phase of the cell cycle (Marjanovic et al 1988). Further, studies of cell proliferation in the absence of glucose have demonstrated that HeLa cells, mouse L cells, early passage human fibroblasts, and primary chicken embryo fibroblasts can all grow indefinitely at near maximal growth rates without glucose as long as they are supplemented with glutamine and uridine (Wice et al 1981).…”
Section: A Major Function Of Aerobic Glycolysis Is To Support Macromosupporting
confidence: 64%
“…When present, the final concentrations of Concanavalin A and PMA were 6/~g/ml and 25 ng/ ml, respectively [10]. DNA synthesis was measured by the 4 hr incorporation of (3H)thymidine (spec.act.…”
Section: Methodsmentioning
confidence: 99%
“…DNA synthesis was measured by the 4 hr incorporation of (3H)thymidine (spec.act. 18 Ci/mmole, Amersham) [10]. L D H isozymes were separated on cellulose acetate plates (Titan III, Helena Laboratories) and stained as in [8].…”
Section: Methodsmentioning
confidence: 99%
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