EFFECT OF PHOSPHATE ION AND 2,4-DINITROPHENOL ON THE ACTIVITY OF INTACT CELLS OF
            <i>THIOBACILLUS FERROOXIDANS</i>

Beck, James S.; Shafia, Fred

doi:10.1128/jb.88.4.850-857.1964

Cited by 46 publications

(11 citation statements)

References 6 publications

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“…This is not surprising, since it is known that a substantial amount of ATP is used by T. ferrooxidans for CO 2 fixation [20]. These results support the previous observations regarding a lower bioleaching activity for T. ferrooxidans in the absence of phosphate [9,10].…”

Section: Resultssupporting

confidence: 90%

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Response of Thiobacillus ferrooxidans to phosphate limitation

Seeger

1993

FEMS Microbiology Reviews

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Phosphate ion is an essential nutrient for all cells. Consequently, starvation for this component may constitute a stressing condition which affects the bioleaching capacity of the biomining microorganisms. Therefore, we have studied the manner in which the chemolithotroph Thiobacillus ferrooxidans responds to phosphate limitation. Under these circumstances the bacteria reduced its growth rate, capacity to oxidize ferrous iron and to fix CO,. Concomitant with these changes, the cells showed an increased synthesis of several proteins, some of which were exclusively synthesized during phosphate starvation. When intact cells grown in the absence of phosphate were labelled with z251, several proteins were iodinated in addition of those observed in control cells, suggesting that the lack of phosphate induces some proteins located in the membranes or the periplasmic space of the bacteria. It is expected that by measuring the levels of expression of some of the proteins induced by the shortage of phosphorus, it might be possible to estimate in situ the relative physiological condition of the bacteria in a given bioleaching operation.

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Section: Resultssupporting

confidence: 90%

“…Phosphate ion (Pi) is an essential nutrient for all living organisms. Therefore, the lack of phosphorus may be a stressing condition which can affect the bioleaching of minerals [9,10].…”

Section: Introductionmentioning

confidence: 99%

Response of Thiobacillus ferrooxidans to phosphate limitation

Seeger

1993

FEMS Microbiology Reviews

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“…This higher efficiency corresponds t o about 4.5 millimoles CO, fixed per mole of Fe2+ oxidized, corresponding t o 54 mg C0,-carbon or a dry weight yield (50% carbon) of 108 mg bacteria. BECK and SHAFIA (1964) obtained growth yields of 100 mg dry wt per mole FeSO,, indicating comparable efficiencies of GO,-fixation t o operate during growth. I n cultures exposed t o normal air, the amounts of 0, and CO, dissolved a t 30 "C may be calculated for pure water as the product of the BUNSEN absorption coefficient and actual volume percentage of each gas present.…”

mentioning

confidence: 77%

“…It is thus not necessary to invoke special adaptation in these organisms to enable them t o survive in conditions of low dissolved CO,, for although dissolved (30:and HCO, will be a t very low levels, they must be formed immediately when dissolved CO, enters the cell cytoplasm, which is a t the more nearly neutral pH a t which the enzymes are known t o function (GALE and BECK 1967). BECK and SHAFIA (1964) demonstrated that C0,-fixation by non-growing T . ferrooxidans a t pH 2.7 was markedly affected by the partial pressure of CO, in WAR-BURG flasks, such that 0.7 ymoles CO, were fixed per 100 ymoles 0, consumed with ea.…”

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confidence: 99%

Biology of Thiobacillus ferrooxidans in relation to the microbiological leaching of sulphide ores

Tuovinen

Kelly

1972

J of Basic Microbiology

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Although chemolithotrophy in bacteria using iron oxidation as a source of energy for CO, assimilation and growth was proposed by WINOGRADSKY (1888) during the earliest studies of autotrophic bacteria, the existence of such bacteria was subsequently seriously doubted (BAAS BECKING and PARKS 1927, LEES 1955). I n the past 20 years, studies on Thiobacillus ferrooxidans have demonstrated beyond all doubt that the oxidation of ferrous to ferric iron can supply all the energy needed by it to support growth. These bacteria require low pII and generally exhibit a remarkable tolerance to dissolved metal ions. As well as oxidizing Fe2+ they will also oxidize inorganic sulphur compounds and can thus be classified as thiobacilli. Three distinct names are, however, used in the literature : Thiobacillus ferrooxidans, Ferrobacillus ferrooxidans and F . sulfooxidans (TEMPLE and COLMER 1951, LEATHEN et al. 1956, KINSEL 1960. I n our view, supported by a number of published comments, all types are basically the same, and the name given to the earliest named iron-oxidizer, Thiobacillus ferrooxidans HINKLE 1947, TEMPLE andCOLMER 1951) is the only correct one for all the "ferrobacilli", and is used in this paper to describe all the strains used hitherto.Our aim in this paper is to review current views and recent advances relating to the physiology and ecology of T . ferrooxidans and to consider these facts in relation to its role in mineral leaching and other biotechnological applications in industrial metallurgy.

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“…Reducing power required is supplied through energy-requiring reversed electron flow [6][7][8]. The oxidation of ferrous iron is the only energy-generating step in the growth of the organism, but instead of being stimulated by uncouplers as in mitochondria it is actually inhibited by uncouplers [9]. The oxidation is also inhibited by permeant anions and weak acids which dissipate Dp by affecting Dw and DpH [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Effect of uncouplers on endogenous respiration and ferrous iron oxidation in a chemolithoautotrophic bacterium Acidithiobacillus (Thiobacillus) ferrooxidans

CHEN

2004

FEMS Microbiology Letters

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Oxidation of ferrous iron (Fe2+) to ferric iron (Fe3+) with oxygen (O2) by Acidithiobacillus (Thiobacillus) ferrooxidans is considered to be inhibited by uncouplers. Oxidation of the endogenous substrates (presumably NADH) with O2 or Fe3+, on the other hand, was stimulated by uncouplers, 2,4-dinitrophenol (DNP) and carbonylcyanide-m-chlorophenyl-hydrazone (CCCP), as expected in respiratorily controlled mitochondria or heterotrophic bacteria. Amytal and rotenone were inhibitory. Fe3+ reduction by endogenous substrates was studied extensively and was found to be stimulated by a permeable anion, SCN- and weak acids, as well as the above uncouplers. Proton translocating properties of some of these stimulators were shown by following a pH change in the cell suspension. It was concluded that any compounds that destroy proton electrochemical gradient, Deltap, stimulated endogenous respiration. Stimulation of Fe2+ or ascorbate oxidation by lower concentrations of uncouplers was successfully demonstrated by shortening the reaction time, but only to a small extent. Uncouplers at concentrations stimulatory to endogenous respiration inhibited Fe2+ oxidation if present before Fe2+ addition. The inhibition by 10 microM CCCP was reversed by washing the cells in a buffer. Complex I inhibitors, atabrine, rotenone and amytal inhibited Fe2+ oxidation, more strongly in the presence of 0.1 mM DNP. It is proposed that Fe2+ oxidation required Deltap perhaps to climb an energetically uphill reaction or to reduce NAD+ to NADH by reversed electron flow for CO2 fixation. The latter interpretation implies some obligatory coupling between Fe2+ oxidation and NAD+ reduction.

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EFFECT OF PHOSPHATE ION AND 2,4-DINITROPHENOL ON THE ACTIVITY OF INTACT CELLS OF THIOBACILLUS FERROOXIDANS

Cited by 46 publications

References 6 publications

Response of Thiobacillus ferrooxidans to phosphate limitation

Response of Thiobacillus ferrooxidans to phosphate limitation

Biology of Thiobacillus ferrooxidans in relation to the microbiological leaching of sulphide ores

Effect of uncouplers on endogenous respiration and ferrous iron oxidation in a chemolithoautotrophic bacterium Acidithiobacillus (Thiobacillus) ferrooxidans

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