Effect of photobiomodulation therapy on radiodermatitis in a mouse model: an experimental animal study

Park, Ji-Hye; Byun, Hyun Jeong; Kim, Hyun Je; Oh, Se Jin; Choi, Changhoon; Noh, Jae Myung; Oh, Dongryul; Lee, Jonghee; Lee, Dong‐Youn

doi:10.1007/s10103-020-03123-x

Cited by 14 publications

(12 citation statements)

References 39 publications

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“…However, it could distinguish between three different groups: low dose (3 and 4.4 Gy/f), intermediate dose (5-6.5 Gy/f) and high dose (7.5 and 8.5 Gy/f). The macroscopic and microscopic changes seen in the skin of the lower lip were compatible with radiation dermatitis reported in mouse skin after SD [33] and fractionated irradiation with fewer fractions than we used [7,14,34,35]. Even though the irradiation protocol and doses differ between the present work and earlier studies, the histological changes in the skin are similar.…”

Section: Discussionsupporting

confidence: 87%

A preclinical model to investigate normal tissue damage following fractionated radiotherapy to the head and neck

Juvkam

Zlygosteva

Arous

et al. 2022

Journal of Radiation Research

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Radiotherapy (RT) of head and neck (H&N) cancer is known to cause both early- and late-occurring toxicities. To better appraise normal tissue responses and their dependence on treatment parameters such as radiation field and type, as well as dose and fractionation scheme, a preclinical model with relevant endpoints is required. 12-week old female C57BL/6 J mice were irradiated with 100 or 180 kV X-rays to total doses ranging from 30 to 85 Gy, given in 10 fractions over 5 days. The radiation field covered the oral cavity, swallowing structures and salivary glands. Monte Carlo simulations were employed to estimate tissue dose distribution. The follow-up period was 35 days, in order to study the early radiation-induced effects. Baseline and post irradiation investigations included macroscopic and microscopic examinations of the skin, lips, salivary glands and oral mucosa. Saliva sampling was performed to assess the salivary gland function following radiation exposure. A dose dependent radiation dermatitis in the skin was observed for doses above 30 Gy. Oral mucositis in the tongue appeared as ulcerations on the ventral surface of the tongue for doses of 75–85 Gy. The irradiated mice showed significantly reduced saliva production compared to controls. In summary, a preclinical model to investigate a broad panel of normal tissue responses following fractionated irradiation of the H&N region was established. The optimal dose to study early radiation-induced effects was found to be around 75 Gy, as this was the highest tolerated dose that gave acute effects similar to that observed in cancer patients.

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Section: Discussionsupporting

confidence: 87%

A preclinical model to investigate normal tissue damage following fractionated radiotherapy to the head and neck

Juvkam

Zlygosteva

Arous

et al. 2022

Journal of Radiation Research

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show abstract

“…However, it could distinguish between 3 different groups: low dose (3 and 4.4 Gy/f), intermediate dose (5-6.5 Gy/f), and high dose (7.5 and 8.5 Gy/f). The macroscopic and microscopic changes seen in the skin of the lower lip were compatible with radiation dermatitis reported in mouse skin after SD (33) and fractionated irradiation with fewer fractions than we used (7,14,34,35). Even though the irradiation protocol and doses differ between the present work and earlier studies, the histological changes in the skin are similar.…”

Section: Discussionsupporting

confidence: 87%

A preclinical model to investigate normal tissue damage following fractionated radiotherapy to the head and neck

Juvkam

Zlygosteva

Arous

et al. 2022

Preprint

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Radiotherapy of head and neck cancer is known to cause both early and late-occurring toxicities. To better appraise normal tissue responses and their dependence on treatment parameters such as radiation field and type, as well as dose and fractionation scheme, a preclinical model with relevant endpoints is required. 12-week old female C57BL/6J mice were irradiated with 100 or 180 kV X-rays to total doses ranging from 30 to 85 Gy, given in 10 fractions over 5 days. The radiation field covered the oral cavity, swallowing structures, and salivary glands. Monte Carlo simulations were employed to estimate tissue dose distribution. The follow-up period was 35 days, in order to study the early radiation-induced effects. Baseline and post irradiation investigations included macroscopic and microscopic examinations of the skin, lips, salivary glands, and oral mucosa. Saliva sampling was performed to assess the salivary gland function following radiation exposure. A dose dependent radiation dermatitis in the skin was observed for doses above 30 Gy. Oral mucositis in the tongue appeared as ulcerations on the ventral surface of the tongue for doses of 75-85 Gy. The irradiated mice showed significantly reduced saliva production compared to controls. In summary, a preclinical model to investigate a broad panel of normal tissue responses following fractionated irradiation of the head and neck region was established. The optimal dose to study early radiation-induced effects was found to be around 75 Gy, as this was the highest tolerated dose that gave acute effects similar to what is observed in cancer patients.

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“…Previous studies have shown that application of PBM increases adenosine‐3′,5′‐cyclic‐ monophosphate and reduces tumor necrosis factor levels, increases peroxisome proliferator‐activated receptors activity and synthesis of heat shock protein 70, inhibition of prostaglandin E2 production and cyclooxygenase messenger RNA expression 34–36 . PBM also decreases intracellular reactive oxygen species.…”

Section: Discussionmentioning

confidence: 99%

Photobiomodulation with polychromatic light (600–1200 nm) improves fat graft survival by increasing adipocyte viability, neovascularization, and reducing inflammation in a rat model

et al. 2021

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Objectives Unpredictability with the final volume and viability of the graft are the major concerns in fat grafting. An experimental study was conducted to increase graft retention using photobiomodulation (PBM) with polychromatic light in near‐infrared region (600–1200 nm) by utilizing its stimulatory effects on angiogenesis, neovascularization, adipocyte viability, and anti‐inflammatory properties. Methods A total of 24 rats were divided into four groups (n = 6) according to the applied polychromatic light protocol to the recipient site (none, before fat transfer, after fat transfer, and combined). In all groups, inguinal fat pad was excised, measured for volume and weight, and transferred to the dorsum of the rat. At the end of the experiment, fat grafts were harvested from the recipient site for volume and weight measurements, histological, and immunohistochemical evaluation. Results Intergroup comparison revealed that fat graft retention regarding weight and volume, was significantly superior in Group IV (p = 0.049 and p = 0.043, respectively), which polychromatic light was applied both before and after transfer of the graft. Hematoxylin–eosin and Masson's trichrome stained sections showed absence of necrosis, fibrosis, inflammation, cyst formation, and increased vascularization of both inner and outer zones of the grafts in Group IV. Also, immunohistochemical staining scores for perilipin (indicator for adipocyte viability), CD31 and VEGF (indicators for angiogenesis and neovascularization) were significantly higher (p < 0.001). Ki67 scores were significantly lower in this group because of anti‐inflammatory environment (p < 0.001). Conclusions Application of PBM to the recipient site before and after fat transfer improved outcomes in rats at 56 day after fat grafting by means of volume retention, increased neovascularization and adipocyte viability and reduced necrosis, fibrosis and inflammation.

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Effect of photobiomodulation therapy on radiodermatitis in a mouse model: an experimental animal study

Cited by 14 publications

References 39 publications

A preclinical model to investigate normal tissue damage following fractionated radiotherapy to the head and neck

A preclinical model to investigate normal tissue damage following fractionated radiotherapy to the head and neck

A preclinical model to investigate normal tissue damage following fractionated radiotherapy to the head and neck

Photobiomodulation with polychromatic light (600–1200 nm) improves fat graft survival by increasing adipocyte viability, neovascularization, and reducing inflammation in a rat model

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