Effect of photodynamic antimicrobial chemotherapy on Candida albicans in the presence of glucose

Oliveira-Silva, Tamires; Alvarenga, Letícia Heineck; Lima-Leal, Cintia; Godoy-Miranda, Bianca; Carribeiro, Pamela; Suzuki, Luis C.; Ribeiro, Martha S.; Kato, Ilka Tiemy; Pavani, Christiane; Prates, Renato Araújo

doi:10.1016/j.pdpdt.2019.05.032

Cited by 12 publications

(3 citation statements)

References 24 publications

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“…It seems that adding glucose along with 1 to yeast suspension increases the uptake of 1, thus increasing the effectiveness of PDT. However, de Oliveira-Silva et al came to the opposite conclusions [55]. In their study, the effect of glucose on 1 uptake by C. albicans was negligible and did not affect the efficacy of the PDT.…”

Section: Methylene Bluementioning

confidence: 81%

“…In their study, the effect of glucose on 1 uptake by C. albicans was negligible and did not affect the efficacy of the PDT. On the other hand, the researchers used a fivefold lower concentration (100 µM) of the PS and exposed the cells to glucose for 120 min (instead of 90 min) [55]. Chitosan was another carbohydrate that was investigated to influence the effectiveness of PACT using 1 [113].…”

Section: Methylene Bluementioning

confidence: 99%

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Photosensitizers Mediated Photodynamic Inactivation against Fungi

Ziental

Mlynarczyk

Czarczyńska-Goślińska

et al. 2021

Nanomaterials

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Superficial and systemic fungal infections are essential problems for the modern health care system. One of the challenges is the growing resistance of fungi to classic antifungals and the constantly increasing cost of therapy. These factors force the scientific world to intensify the search for alternative and more effective methods of treatment. This paper presents an overview of new fungal inactivation methods using Photodynamic Antimicrobial Chemotherapy (PACT). The results of research on compounds from the groups of phenothiazines, xanthanes, porphyrins, chlorins, porphyrazines, and phthalocyanines are presented. An intensive search for a photosensitizer with excellent properties is currently underway. The formulation based on the existing ones is also developed by combining them with nanoparticles and common antifungal therapy. Numerous studies indicate that fungi do not form any specific defense mechanism against PACT, which deems it a promising therapeutic alternative.

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Section: Methylene Bluementioning

confidence: 81%

Section: Methylene Bluementioning

confidence: 99%

Photosensitizers Mediated Photodynamic Inactivation against Fungi

Ziental

Mlynarczyk

Czarczyńska-Goślińska

et al. 2021

Nanomaterials

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“…Ferreira et al and de Oliveira-Silva et al achieved different reductions of C. albicans , 0.5 log 10 and 6 log 10 respectively, with 32 μg/mL of MB and a fluence of 30 J/cm 2 for 3–4 min. This shows the variability between experiments [ 29 , 30 ]. The results of Ferreira et al are closer to those of this work (32 μg/mL of MB does not produce complete photoinactivation) ( Table 2 ).…”

Section: Discussionmentioning

confidence: 99%

In Vitro Effect of Photodynamic Therapy with Different Lights and Combined or Uncombined with Chlorhexidine on Candida spp.

et al. 2021

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Candidiasis is very common and complicated to treat in some cases due to increased resistance to antifungals. Antimicrobial photodynamic therapy (aPDT) is a promising alternative treatment. It is based on the principle that light of a specific wavelength activates a photosensitizer molecule resulting in the generation of reactive oxygen species that are able to kill pathogens. The aim here is the in vitro photoinactivation of three strains of Candida spp., Candida albicans ATCC 10231, Candida parapsilosis ATCC 22019 and Candida krusei ATCC 6258, using aPDT with different sources of irradiation and the photosensitizer methylene blue (MB), alone or in combination with chlorhexidine (CHX). Irradiation was carried out at a fluence of 18 J/cm2 with a light-emitting diode (LED) lamp emitting in red (625 nm) or a white metal halide lamp (WMH) that emits at broad-spectrum white light (420–700 nm). After the photodynamic treatment, the antimicrobial effect is evaluated by counting colony forming units (CFU). MB-aPDT produces a 6 log10 reduction in the number of CFU/100 μL of Candida spp., and the combination with CHX enhances the effect of photoinactivation (effect achieved with lower concentration of MB). Both lamps have similar efficiencies, but the WMH lamp is slightly more efficient. This work opens the doors to a possible clinical application of the combination for resistant or persistent forms of Candida infections.

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