Effect of Photomodulation Therapy on Development of Oxidative Stress in Blood Leukocytes of Rats with Streptozocin-Induced Diabetes Mellitus

Karmash, O. I.; Liuta, M. Ya.; Korobov, A. M.; Sybirna, N. O.

doi:10.3103/s0095452720050114

Cited by 3 publications

(1 citation statement)

References 35 publications

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“…3). In our previous research, we revealed similar results for catalase and superoxide dismutase activity in leukocyte lysates [8]. We concluded that positive effects of PBM therapy occured not because of its influence on protoporphyrin, as we did not find significant changes in protoporphyrin content under irradiation.…”

Section: рис 1 типові криві дисоціації оксигемоглобіну щурів за досліджуваних умовsupporting

confidence: 81%

The effect of photobiomodulation therapy of some indices of rats’ blood cells functional state under experimental diabetes mellitus

et al. 2021

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Background. Diabetes mellitus is a chronic endocrine-metabolic disease caused by an absolute or relative insulin deficiency. During diabetes, there are perfect conditions for the development of oxidative stress: the content of substrates for oxidation increases, the content of natural antioxidants decreases and the activity of antioxidant systems is suppressed. It is known that photobiomodulation therapy produce antioxidant and antihyperglycemic effects. Here we investigated its influence on blood system functioning. Materials and Methods. The study was performed on male Wistar rats. Experimental diabetes mellitus was induced by the intraperitoneal injection of streptozotocin. Leukocyte formula was calculated using blood smears stained by Romanowsky–Giemsa. Catalase activity was determined spectrophotometrically. Affinity of hemoglobin to oxygen was evaluated by spectrophotometric method in Ivanov’s modification by drawing oxygenation curves. The protoporphyrin content in whole blood was measured by analyzing its fluorescence spectra. The content of NO2-, total and inducible NO synthase activity was determined spectrophotometrically. Results. Under the action of photobiomodulation therapy on healthy animals, there was a shift of oxygenation curves to the left and a decrease of P50, whereas under irradiation of rats with diabetes, there was a shift of oxygenation curves to the right and increase in P50 compared to indices in nonirradiated animals. During diabetes, there was a decrease in protoporphyrin content compared to control, but there was a tendency to increase under photobiomodulation. Photobiomodulation therapy of rats with diabetes increased catalase activity in erythrocyte hemolysates. We revealed significant changes in leukocyte formula under photobiomodulation. The total NO synthase activity in leukocytes of rats with diabetes was higher compared to healthy animals, but decreased under the action of photobiomodulation. We found an increase in inducible NO synthase activity in leukocytes of rats with diabetes and in leukocytes of irradiated healthy animals. An increase in NO2- content in leukocytes of rats with diabetes was detected. Under photobiomodulation, NO2- content was significantly lower in rats with diabetes. Conclusion. Photobiomodulation therapy produces a corrective action on blood system during diabetes, in particular, it improves oxygen release from hemoglobin and prevents hypoxia. Simultaneously with the increase in tissue oxygen saturation, a decrease in NO synthase activity and nitrite content along with an increase in catalase activity prevents the development of oxidative stress.

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Section: рис 1 типові криві дисоціації оксигемоглобіну щурів за досліджуваних умовsupporting

confidence: 81%

The effect of photobiomodulation therapy of some indices of rats’ blood cells functional state under experimental diabetes mellitus

et al. 2021

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Effect of photobiomodulation therapy on the regulation of glucose uptake by lymphocytes in diabetes mellitus (Review)

2021

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For most cells, including lymphocytes, glucose is a primary energy source, and, therefore, it is vital to understand the regulatory mechanisms that control the work of glucose transporters. Lymphocytes are pivotal for mediation of immune and inflammatory responses. A feature of lymphocytes is increasing glucose utilization during activation of the immune function, which is strongly dependent on glucose uptake. Some studies show that elevated glucose concentration in diabetes mellitus affects lymphocytes’ glucose transporters expression, whichcorrelates with impaired immune functions and may become one of the predisposing factors of contracting infectious diseases. Recent studies have focused on glucose transporters as therapeutic targets for a variety of diseases, including diabetes mellitus. This review demonstrates the effect of photobiomodulationtherapy on glucose uptake by Na+-coupled glucose carrier SGLT1 and facilitated diffusion glucose carriers of the GLUT family (GLUT1, GLUT3, GLUT4) in normal and diabetic lymphocytes.

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Photobiomodulation therapy protects red blood cells against nitrative stress during streptozotocin-induced diabetes

Maslakova¹,

Liuta²

2022

Biol. Stud.

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Background. According to the International Diabetes Federation Diabetes Atlas, 10th edition, diabetes is responsible for 6.7 million deaths in 2021. Diabetes mellitus is a group of metabolic disorders characterized by hyperglycemia secondary to either resistance to insulin, insufficient insulin secretion, or both. Oxidative and nitrative stress is a vital part of the complex mechanism by which diabetes and its complications develop. It is known that Photobiomodulation therapy accelerates diabetic wound healing, treats relegated inflammation, and increases oxygen availability for cells. Although some basic molecular mechanisms caused by photobiomodulation therapy in different cell types are already known, they have not been studied in erythrocytes and are different due to the absence of central organelles such as nucleus and mitochondria. The aim of the study was to investigate the effect of photobiomodulation therapy on the development of nitrative stress in blood plasma and erythrocytes of rats from different experimental groups. Materials and Methods. The study was performed on white outbred male rats weighing 130–180 g. The diabetes mellitus was induced by intraperitoneal injection of streptozotocin (60 mg/kg). Rats were exposed to photobiomodulation with light-emitting diodes at a wavelength of 630–660 nm daily for 10 days. The irradiation time was 5 minutes. The content of nitrite and nitrate anions, total NO synthase activity, as well as the activity of its endothelial and inducible isoforms in red blood cells of rats were determined spectrophotometrically. Results and Discussion. Under streptozotocin-induced diabetes mellitus, the content of nitrite and nitrate anions and NO synthase activity increased in the rats’ red blood cells, as well as in blood plasma. Moreover, we found an increase in inducible NO synthase activity and nitrate ion content in red blood cells of irradiated healthy rats. Also, there was an increase in nitrite and nitrate ion content after photobiomodulation therapy in the blood plasma of healthy animals. On the other hand, irradiation caused a decrease in NO synthase activity with a parallel reduction in both nitrite and nitrate anions content in erythrocytes and blood plasma of rats with experimental diabetes. Conclusion. Photobiomodulation therapy protects rats’ red blood cells from nitrative stress during streptozotocin-induced diabetes mellitus.

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Effect of Photomodulation Therapy on Development of Oxidative Stress in Blood Leukocytes of Rats with Streptozocin-Induced Diabetes Mellitus

Cited by 3 publications

References 35 publications

The effect of photobiomodulation therapy of some indices of rats’ blood cells functional state under experimental diabetes mellitus

The effect of photobiomodulation therapy of some indices of rats’ blood cells functional state under experimental diabetes mellitus

Effect of photobiomodulation therapy on the regulation of glucose uptake by lymphocytes in diabetes mellitus (Review)

Photobiomodulation therapy protects red blood cells against nitrative stress during streptozotocin-induced diabetes

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