Effect of phylloquinone on indomethacin‐induced gastric ulceration in rats: Role of SIRT‐1

Abstract

Gastric ulcer is the most common gastrointestinal disorder affecting people globally.Although many drugs are available to treat ulcers, the mortality rate is relatively high, and drugs lack selectivity to treat ulcers without causing side effects. In this study, the potential therapeutic effects of phylloquinone were tested against indomethacininduced gastric ulcer in rats by giving rats a single oral dose of indomethacin (48 mg/kg), followed by phylloquinone (10 mg/kg) orally, once daily for six consecutive days. Phylloquinone significantly attenuated indomethacin-induced oxidative and inflammatory responses through hindering the inflammatory cascade by decreasing the levels of TNF-α, NF-κB, INOS and COX-2 which counteracts indomethacin effects. Also, it increased NAD + which enhanced SIRT-1 level. Furthermore, phylloquinone was effective in increasing mucus secretion, decreasing acid secretion, reversing histological effects caused by indomethacin and minimizing ulcer and lesion indices All these findings indicate that phylloquinone may be used in protection and treatment of indomethacin-induced gastric ulcer.

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“…AT extract has also exhibited an anti-inflammatory effect that causes significant decrease in TNF-α and IL-6 levels by stimulating prostaglandin. From histo-architectural point of view it may be stated that significant damage and erosion was noted in mucosa and submucosa layer of stomach tissue in all the indomethacin treated groups and severe lesion observed in severe ulcerated groups 48 . Hydro-methanol extract of AT treatment gives significant protection.…”

Histological and Immunohistochemical analysis of possible ameliorative effect of Ashwagandha on Methotrexate-Induced Oral Mucositis in Albino Rats

“…First, the sections were deparaffinized, rehydrated, and placed for 10 minutes in a 3% H 2 O 2 solution at room temperature, then immersed in an antigen retrieval solution. Next, the sections were incubated in 10% normal goat serum in phosphate buffered saline solution (PBS) to prevent non-specific protein binding, followed by the addition of ki67 primary antibody (Dako, M7240, 1:100) (20) or COX2 primary antibody (cat, RB-9072-P0) (21) Lab Vision, USA and incubated in a moist chamber overnight at 4 ̊C. Further, drops of streptavidin peroxidase were added for 30 minutes then rinsed in PBS.…”