Spinning represents the foundational step in textile manufacturing, with yarn quality significantly influencing subsequent processes. Consequently, enhancing yarn quality remains a primary concern for spinners. Of various spinning techniques, ring spinning stands out for its blend of productivity and quality. The final ring yarn is produced by the ring frame, a machine encompassing multiple functional zones. A pivotal component within this frame is the spacer between the top and bottom aprons in the drafting zone. It modulates drafting pressure and fibre movement, directly affecting yarn quality. This research explored the impact of spacer size on yarn quality by employing seven distinct spacer sizes to produce 20 Ne cotton combed yarn. Results indicated that smaller spacer sizes often yielded inferior yarn quality. As the size increased, quality improved; however, after reaching an optimal size, further enlargement led to a decline in five key quality metrics: CVm (%), IPI, Hairiness, Tenacity, and Elongation %. This fluctuation can be attributed to changes in fibre movement and drafting pressure in the drafting zone. The study employed Interpretive Structural Modelling (ISM), a methodology grounded in expert opinions, to rank these quality parameters in importance. Through ISM calculations, yarn tenacity emerged as the most crucial quality parameter, aligning with its significance in producing woven fabrics. Furthermore, a Cronbach's Alpha score of 0.66 underscores the robust internal consistency and interrelation attesting to the study's reliability.