Effect of plant biostimulants on root and plant health and the rhizosphere microbiome of citrus trees in huanglongbing-endemic conditions

Castellano‐Hinojosa, Antonio; Meyering, Bo; Nuzzo, Andrea; Strauss, Sarah L.; Albrecht, Ute

doi:10.1007/s00468-021-02133-8

Cited by 20 publications

(7 citation statements)

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“…Fibrous roots for microbial analyses were placed in 50-mL sterile centrifuge tubes, immediately flash frozen in liquid nitrogen, and stored at −80° until analysis. Rhizosphere soil for RNA extraction was isolated using sterile phosphate-buffered saline (PBS) solution as described previously [ 32 ].…”

Section: Methodsmentioning

confidence: 99%

“…Citrus is a globally important perennial fruit crop, but its production faces challenges, particularly from the devastating disease HLB [ 26 – 28 ]. Several strategies, including the use of selected rootstock genotypes [ 9 , 29 ], ground application of specific nutrients [ 30 ], and soil amendments (e.g., compost and plant biostimulants such as humic substances, seaweed extracts, and microbial inoculants) [ 31 , 32 ], have been proposed to improve root health and crop production in citrus. In addition, there is increased interest to understand the composition and function of the citrus microbiome to help optimize and maximize future agricultural microbiome engineering solutions [ 33 – 35 ].…”

Section: Introductionmentioning

confidence: 99%

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Interactions between rootstocks and compost influence the active rhizosphere bacterial communities in citrus

2023

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Background While the rootstock genotype (belowground part of a plant) can impact rhizosphere microbial communities, few studies have examined the relationships between rootstock genotype-based recruitment of active rhizosphere bacterial communities and the availability of root nutrients for plant uptake. Rootstocks are developed to provide resistance to disease or tolerance of abiotic stresses, and compost application is a common practice to also control biotic and abiotic stresses in crops. In this field study, we examined: (i) the effect of four citrus rootstocks and/or compost application on the abundance, diversity, composition, and predicted functionality of active rhizosphere bacterial communities, and (ii) the relationships between active rhizosphere bacterial communities and root nutrient concentrations, with identification of bacterial taxa significantly correlated with changes in root nutrients in the rhizosphere. Results The rootstock genotype determined differences in the diversity of active rhizosphere bacterial communities and also impacted how compost altered the abundance, diversity, composition, and predicted functions of these active communities. Variations in the active bacterial rhizobiome were strongly linked to root nutrient cycling, and these interactions were root-nutrient- and rootstock-specific. Direct positive relationships between enriched taxa in treated soils and specific root nutrients were detected, and potentially important taxa for root nutrient uptake were identified. Significant differences in specific predicted functions were related to soil nutrient cycling (carbon, nitrogen, and tryptophan metabolisms) in the active bacterial rhizobiome among rootstocks, particularly in soils treated with compost. Conclusions This study illustrates that interactions between citrus rootstocks and compost can influence active rhizosphere bacterial communities, which impact root nutrient concentrations. In particular, the response of the rhizobiome bacterial abundance, diversity, and community composition to compost was determined by the rootstock. Specific bacterial taxa therefore appear to be driving changes in root nutrient concentrations in the active rhizobiome of different citrus rootstocks. Several potential functions of active bacterial rhizobiomes recruited by different citrus rootstocks did not appear to be redundant but rather rootstock-specific. Together, these findings have important agronomic implications as they indicate the potential for agricultural production systems to maximize benefits from rhizobiomes through the choice of selected rootstocks and the application of compost.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Interactions between rootstocks and compost influence the active rhizosphere bacterial communities in citrus

2023

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“…Additionally, Wagner et al (2014) confirmed that rhizosphere microorganisms can affect flowering phenology and selection on flowering time. There is growing evidence of the ability of rhizosphere microorganisms to alter the root morphological structure and improve root functions, which in turn improves plant nutrient uptake and physiology (Vacheron et al, 2013;Castellano-Hinojosa et al, 2021). Furthermore, some rhizosphere microorganisms can also severely constrain plant growth and development.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the diversity and structure of the rhizosphere microbial community between the straight and twisted trunk types of Pinus yunnanensis

Gan

et al. 2023

Front. Microbiol.

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BackgroundPinus yunnanensis is a major silvicultural species in Southwest China. Currently, large areas of twisted-trunk Pinus yunnanensis stands severely restrict its productivity. Different categories of rhizosphere microbes evolve alongside plants and environments and play an important role in the growth and ecological fitness of their host plant. However, the diversity and structure of the rhizosphere microbial communities between P. yunnanensis with two different trunk types—straight and twisted—remain unclear.MethodsWe collected the rhizosphere soil of 5 trees with the straight and 5 trees with the twisted trunk type in each of three sites in Yunnan province. We assessed and compared the diversity and structure of the rhizosphere microbial communities between P. yunnanensis with two different trunk types by Illumina sequencing of 16S rRNA genes and internal transcribed spacer (ITS) regions.ResultsThe available phosphorus in soil differed significantly between P. yunnanensis with straight and twisted trunks. Available potassium had a significant effect on fungi. Chloroflexi dominated the rhizosphere soils of the straight trunk type, while Proteobacteria was predominant in the rhizosphere soils of the twisted trunk type. Trunk types significantly explained 6.79% of the variance in bacterial communities.ConclusionThis study revealed the composition and diversity of bacterial and fungal groups in the rhizosphere soil of P. yunnanensis with straight and twisted trunk types, providing proper microbial information for different plant phenotypes.

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“…As much as 30%–50% of root density is lost to the disease before the above‐ground symptoms even appear (Graham et al, 2013; Johnson et al, 2014). These observations laid the foundations for near‐term therapies that specifically reduce the levels of the pathogen in infected citrus trees and reverse disease impacts by improving root and soil health (Castellano‐Hinojosa et al, 2021; Johnson & Graham, 2015; Strauss & Albrecht, 2018; Stokes et al, 2020). Agronomic management practices impact soil health, alter soil microbiome, and activate natural biocontrol mechanisms that lead to the development of disease suppressive soils for managing few plant pathogens (Carrión et al, 2019; Ou et al, 2019; Trivedi et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…As much as 30%-50% of root density is lost to the disease before the above-ground symptoms even appear (Graham et al, 2013;Johnson et al, 2014). These observations laid the foundations for near-term therapies that specifically reduce the levels of the pathogen in infected citrus trees and reverse disease impacts by improving root and soil health (Castellano-Hinojosa et al, 2021;Johnson & Graham, 2015;Strauss & Albrecht, 2018;Stokes et al, 2020).…”

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confidence: 99%

Management‐induced shifts in rhizosphere bacterial communities contribute to the control of pathogen causing citrus greening disease

Bazany

Delgado‐Baquerizo

Thompson

et al. 2022

J of Sust Agri & Env

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Introduction Citrus greening (aka Huanglongbing, HLB) caused primarily by the bacterial pathogen Candidatus Liberibacter asiaticus (CLas) has devastating effects on the global citrus industry. Agricultural management‐induced changes in microbial communities are hypothesised to contribute toward HLB resistance by reducing pathogen titre and increasing root and soil health. However, we have a limited understanding of the impacts of management practices on the soil microbiome, making the extent of HLB management uncertain. Material and Methods Here we investigated the effect of agricultural management practices on reducing CLas titer via changes in rhizosphere‐associated bacterial communities. Rhizosphere and root samples were collected from two sites in Florida where different management practices (e.g., metalized reflective mulch ground covers, compost application and microbial inoculations) are currently being implemented to prevent HLB. Management‐induced changes in the rhizosphere bacterial community were assessed using amplicon sequencing. qPCR assays were used to quantify the titer of the pathogen CLas in roots. In addition, we measured soil properties and the activities of microbial enzymes involved in soil nutrient cycling. Results Our results indicated that certain management practices lead to shifts in the community structure of rhizosphere bacterial communities that negatively interact with the HLB pathogen. Management practices improved soil quality and reduced CLas titer. Additionally, we found that Actinobacteria were frequently enriched in the successful treatment sites, suggesting that Actinobacteria taxa could be indicators for HLB suppression properties in the soil. Conclusion Our results suggest that microbiome manipulation, either through changes in the management practices or microbial amendment, can increase the suppressive potential of soils, resulting in the reduction in CLas titer and potentially leading to HLB suppression in citrus groves.

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Effect of plant biostimulants on root and plant health and the rhizosphere microbiome of citrus trees in huanglongbing-endemic conditions

Cited by 20 publications

References 86 publications

Interactions between rootstocks and compost influence the active rhizosphere bacterial communities in citrus

Interactions between rootstocks and compost influence the active rhizosphere bacterial communities in citrus

Comparison of the diversity and structure of the rhizosphere microbial community between the straight and twisted trunk types of Pinus yunnanensis

Management‐induced shifts in rhizosphere bacterial communities contribute to the control of pathogen causing citrus greening disease

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