Effect of plant growth regulator, light and media formulation on initiation and growth of Parkia biglobosa (Jacq.) R. Br. Ex Don callus cultures from cotyledons and mature zygotic embryos

Adesoye, Adenubi; Orkpeh, Uterdzua

doi:10.4314/njhs.v14i1.62160

Cited by 2 publications

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“…The callus appeared healthy and green in color and friable in nature. This finding is in accordance with the results of Adesoye and Orkpeh (2009) and Behbahani et al (2011) who also favored WPM over MS. After 120 days, shoot regeneration was first observed in treatment SI33 (1 mg/l -1 BAP and 0. mg/l 2, 4-D) with 87.50% of callus induction and 75% of callus regeneration frequency.…”

Section: Resultssupporting

confidence: 93%

Expression Pattern of in vitro Organogenesisassociated Genes as Transcriptional Marker in Sandalwood (Santalum album L.) Micropropagation

Pandey

Patil

Modi

et al. 2022

Plant Tissue Cult. & Biotech.

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The world's most expensive wood, sandalwood (Santalum album L.), requires a stringent mass-propagation technique to prevent future scarcity. Plant tissue culture is an efficient method that regenerates the whole plant from a single cell on a hormone-based growth medium. To efficiently regulate the formation of plants, it is important to understand the developmental organogenesis pathways (i.e., direct and indirect) through gene expression studies. Therefore, an effective protocol for the direct and indirect organogenesis of sandalwood was developed, followed by the characterization of gene expression patterns in investigation. Five in vitro organogenesis genes namely, Alternative oxidase (ao), Late embryogenesis abundant (lea), Cytochrome p450 (cyt-p450), ABC transporter (abct), and Serine-threonine phosphatase (stp), were screened from three stages of sandalwood development; (1) inoculated leaf, (2) proliferated leaf shoot/callus formation and (3) shoot formation. The best treatments for plant regeneration in Woody Plant Media (WPM) were SI24 (2.5 mg/l 2,4-D) for indirect organogenesis and SD14 (2.0 mg/l BAP and 0.4 mg/l-1 NAA) for direct organogenesis. During the initial stages of organogenesis, ao, cyt-p450 and abct showed no/little change in expression in the direct pathway however up-regulation of ao and abct and downregulation of cyt-p450 were observed in the indirect pathway. Expression of lea was increased up to 70-fold during direct and dropped to half during indirect organogenesis. The optimization of the sandalwood organogenesis regeneration medium and the identification of distinct gene expression patterns will serve as transcriptional markers for the early prediction of the organogenesis stage, assisting in the sandalwood conservation. Plant Tissue Cult. & Biotech. 32(2): 103-113, 2022 (December)

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Section: Resultssupporting

confidence: 93%

Expression Pattern of in vitro Organogenesisassociated Genes as Transcriptional Marker in Sandalwood (Santalum album L.) Micropropagation

Pandey

Patil

Modi

et al. 2022

Plant Tissue Cult. & Biotech.

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show abstract

“…Among all treatments tested, SI24 (2.5 mgL -1 2,4-D) followed by the treatment SI18 (3 mgL -1 2,4-D and 1 mgL -1 glycine and 25 mgL -1 ADS) and SI33 (1 mgL -1 BAP and 0.5 mgL -1 2,4-D) reflected as the best treatments for achieving early callus induction response after 40 days. This finding is in accordance with the results of Adesoye and Orkpeh (2009) and Behbahani et al (2011) who also supported WPM over MS. Rashmi and Trivedi (2014) postulated that 2,4-D may have encouraged the synthesis of endogenous purines and cytokinins ultimately resulting in higher rates of cell division. The days to callus induction ranged from 35 to 37 days while the callus induction % ranged from 10% to 50%.…”

Section: In Vitro Organogenesissupporting

confidence: 93%

Expression pattern of in vitro organogenesis-associated genes as transcriptional marker in Indian Sandalwood ( Santalum album L.).

et al. 2021

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Indian sandalwood (Santalum album L.) is an expensive wood that requires reproducible method for mass propagation to ensure consistent production and sustainable use of sandalwood. For mass propagation of sandalwood, plant organogenesis requires different combinations of the tissue culture medium. The media is composed of exogenous phytohormones which decides the explant's morphological stages such as shooting or rooting induction. Early prediction of morphological stage from explant can potentially help in selecting the exogenous phytohormones combinations thereby saving time and resources for mass sandalwood propogation. An efficient protocol for the direct and indirect organogenesis (up to shooting development phase) of sandalwood were developed using Woody Plant Media (WPM). WPM supplemented with various concentrations of 6-Bezylaminopurine (BAP) and 1-Naphthaleneacetic acid (NAA) were tested for direct organogenesis, while different treatments consisting of various levels of 2,4-dichlorophenoxyacetic acid (2,4-D), NAA, BAP, Adenine sulphate (ADS), glycine and potassium nitrate were tested for indirect organogenesis. Three stages of leaf development were selected viz., the leaf just after inoculation in WPM media, initial stage of callus formation from leaf and shoot formation for expression pattern analysis. The targeted genes were Alternative oxidase (ao), Late embryogenesis abundant (lea), Cytochrome P450 (cyt-p450), ABC transporter (abct), and Serine-threonine phosphatase (stp) which are associated with in vitro organogenesis. The expression patterns were evaluated to identify a transcription marker. During the initial stages of organogenesis, ao, cyt-p450 and abct showed no/little change in expression in the direct pathway but up-regulation of ao and abct and downregulation of cyt-p450 were observed in the indirect pathway. Expression of lea was increased up to 70-fold during direct and dropped to half during indirect organogenesis.

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Effect of plant growth regulator, light and media formulation on initiation and growth of Parkia biglobosa (Jacq.) R. Br. Ex Don callus cultures from cotyledons and mature zygotic embryos

Cited by 2 publications

References 0 publications

Expression Pattern of in vitro Organogenesisassociated Genes as Transcriptional Marker in Sandalwood (Santalum album L.) Micropropagation

Expression Pattern of in vitro Organogenesisassociated Genes as Transcriptional Marker in Sandalwood (Santalum album L.) Micropropagation

Expression pattern of in vitro organogenesis-associated genes as transcriptional marker in Indian Sandalwood ( Santalum album L.).

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