Effect of plant growth regulators and explant types on induction and growth of callus of <i>Primula veris</i> L.

Morozowska, Maria; Wesołowska, Maria; Głowicka-Wołoszyn, Romana; Kosińska, Anna Magdalena

doi:10.2478/hepo-2013-0013

Cited by 3 publications

(4 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results of this study partially conflicted with Chutipaijit [22] since the callus growth under 24 h light exposure recorded a better callus induction (75%) compared to the callus grown under continuous dark (50%), and 16h/8 light exposure (25%). On the other hand, a study by Ozarowski [23] on tobacco plants reported similar results to this study. The leaf blade of Motihari achieved 97.20% of callus formation under light condition.…”

Section: Anova Analysissupporting

confidence: 90%

See 1 more Smart Citation

The Influence of Plant Growth Regulators and Light Supply on Bitter Cassava Callus Initiation for Starch Production

Mohamad Puad,

Ibrahim,

Azmi

et al. 2024

IIUMEJ

View full text Add to dashboard Cite

The application of cassava starch in the biopolymers industry has been growing significantly due to its low cost, good oxygen barrier in the dry state, and biodegradability. Plant culture technology is an alternative to traditional propagation as it does not require large areas for production, has a higher rate of multiplication, and produces disease-free plants. However, the application of cassava callus culture for starch production is limited. This study focuses on identifying the significant culture parameters for a maximum Rayong cultivar cassava callus growth and evaluation of its starch content. Cassava stems petiole and leaf explants were cultured on MS medium containing different combinations of 2,4-D (8, 12, and 15 mg/L) and BAP (1, 3, and 5 mg/L) under three light conditions (0, 16, and 24 h). The screening of the most influential parameter was done using the 2-level Factorial Design in Design Expert v13 by analyzing the frequency of callus formation. All leaf explant turned brown with no callus induction. The highest frequency of callus formation derived from stem petiole explant was achieved by the combination of 8 mg/L 2,4-D and 1 mg/L BAP under the light condition (75%) followed by 8 mg/L 2,4 D + 1 mg/L BAP under the dark condition (50%). Based on the ANOVA analysis, the individual supply of 2,4-D and BAP respectively, have a negative effect on callus formation while the combination of 2,4-D and BAP has a positive effect. Light supply did not significantly affect cassava callus formation. The amount of starch in the cassava callus was then investigated using an iodine test which yielded 0.21% of the total weight of the callus (0.0101g). The amount of starch is relatively low considering that the callus was not grown under the optimum condition for starch production. The findings of this study open prospects for future research in cassava cultures in favor of starch production. ABSTRAK: Penggunaan kanji ubi kayu dalam industri biopolimer telah meningkat secara mendadak disebabkan oleh faktor kosnya yang rendah, rintangan oksigen yang baik dan sifat keterbiodegradasi. Teknologi kultur tisu tumbuhan merupakan alternatif kepada pembiakan secara tradisional yang mana teknologi ini tidak memerlukan kawasan penanaman yang luas, penghasilan yang lebih singkat dan menghasilkan tumbuhan bebas penyakit. Walau bagaimanapun, kajian mengenai penggunaan kanji dari kultur ubi kayu masih belum meluas. Kajian ini bertujuan mengenal pasti parameter penting dalam pertumbuhan maksimum kalus kultivar ubi kayu Rayong dan kandungan kanjinya. Eksplan dari tangkai petiol dan daun ubi kayu dikulturkan dalam medium MS yang mengandungi pelbagai kombinasi hormon tumbuhan 2,4-D (8, 12 dan 15 mg/L) dan BAP (1, 3 dan 5 mg/L) di bawah tiga tempoh masa pencahayaan (0, 16 dan 24 jam). Saringan parameter paling berpengaruh dilakukan menerusi reka bentuk faktorial 2-peringkat perisian Design Expert v13 melalui analisa frekuensi pembentukan kalus. Semua eksplan daun telah bertukar dari hijau ke perang dan tidak menunjukkan induksi kalus. Bagi eksplan batang daun, frekuensi pembentukan kalus tertinggi diperoleh dari kombinasi 8 mg/L 2,4-D dan 1 mg/L BAP di bawah pencahayaan 24 jam (75%) diikuti dengan 8 mg/L 2,4 D + 1 mg/L BAP di bawah pencahayaan malap (50%). Berdasarkan analisis ANOVA, medium 2,4-D dan BAP masing-masing menunjukkan kesan negatif kepada pembentukan kalus, sementara kombinasi 2,4-D dan BAP memberikan kesan positif. Dapatan ini menunjukkan bahawa pencahayaan tidak signifikan terhadap pembentukan kalus kultur ubi kayu. Kandungan kanji di dalam kalus ubi kayu kemudiannya diuji dengan larutan iodin, dan menunjukkan dapatan sebanyak 0.21% dari berat kalus (0.0101 g). Secara relatif, nilai ini adalah rendah memandangkan eksperimen ini bukan dihasilkan di bawah keadaan optimum bagi pembentukan kanji. Hasil kajian ini membuka peluang kepada kajian-kajian lain di masa hadapan dalam penghasilan kanji dari kultur tisu ubi kayu.

show abstract

Section: Anova Analysissupporting

confidence: 90%

“…The leaf blade of Motihari achieved 97.20% of callus formation under light condition. It was justified that the irregularities of reported results depend on the plant cultivar and type of explant used [23].…”

Section: Anova Analysismentioning

confidence: 99%

The Influence of Plant Growth Regulators and Light Supply on Bitter Cassava Callus Initiation for Starch Production

Mohamad Puad,

Ibrahim,

Azmi

et al. 2024

IIUMEJ

View full text Add to dashboard Cite

show abstract

“…On the other hand, 2,4-D and BA combinations generated friable white callus from leaf explant.Therefore, compact, fragile, greenish, whitish and brownish calli derived from various explants in various combinations and concentrations of growth regulators. However, these types of callus morphology were also found in some other plants like Ipomoea obscura L. (Mungole et al, 2009), Gynura procumbens (Nurokhman et al, 2019). A green compact callus was found in Orthosiphonsta mineus from a balanced concentration of NAA and BA (Elangomathavan et al, 2017).…”

Section: Resultsmentioning

confidence: 96%

Ex-Situ Conservation of An Endangered Medicinal Plant Andrographis Paniculata By Plant Tissue Culture

et al. 2022

View full text Add to dashboard Cite

An effective and rapid in vitro regeneration protocol of Kalmegh (Andrographis paniculata) was established by investigating the factors like combinations of plant growth regulators and explant types (stem, leaf and midrib). To find out the effective medium for callus induction and shoot regeneration, different explants of A. paniculata were cultured on MS media enriched with several concentrations of 6-benzylaminopurine (BA), α-naphthalene acetic acid (NAA) and 2, 4-dichlorophenoxy acetic acid (2,4-D). Stem explant was noticed more responsive than leaf and midrib explant both in callus initiation and shoot regeneration. The ranges of callus initiation from stem, leaf and midrib explants were 26.67 - 100%, 20 - 93.33% and 13.33 - 73.33%, respectively. The calli obtained from midrib explants were not used in shoot initiation because of its poor size. The stem explant exhibited the maximum 73.33% shoot regeneration frequency in a comparison with leaf explants (60%). The maximum callus induction (100%) and shoot regeneration (73.33%) from stem explants were noticed in MS medium strengthened with 0.5 mg/L NAA and 2.0 mg/L BA and half strength MS media complemented with 0.1 mg/L NAA and 3.0 mg/L BA respectively. The highest shoot regeneration from the stem explant may be due to presence of more active parenchymatous cells than that of leaf explant. Half MS medium fortified with 2.0 mg/L IBA considered as best root initiation medium as it resulted in maximum rooting (93.33%). After acclimatization, the plants were transferred to field and found identical to the mother plant.

show abstract

“…It spreads around Giresun, Gümüşhane, Trabzon, and Rize in the Eastern Black Sea region in Türkiye (Anşin et al, 1994). There are many studies on the genus Primula, and morphological (Smith and Fletcher, 1948;Zhang and Kadereit, 2005), cytological (Bruum, 1932), biochemical (Yayli, 2001;Morozowska, 2004;Fico et al, 2007. ), and molecular (Cservenka et al, 2002;Zhang and Kadereit, 2004;Zhang and Kadereit, 2005;Manfield et al, 2005;Gültepe et al, 2010) studies have been carried out.…”

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

Comprehensive assessment of genetic variation in native heterostylous primrose genotypes of Türkiye

TÜTÜNCÜ,

DALDA-SEKERCI,

BULUT

et al. 2023

Not Bot Horti Agrobo

View full text Add to dashboard Cite

Primula vulgaris L. is an essential species of ornamental plant value with attractive flowers distributed in humid and cold regions. It spreads around Giresun, Gümüşhane, Trabzon, and Rize in Türkiye’s Eastern Black Sea region. Through molecular and morphological markers, this study determined the genetic diversity and similarity levels of Primula genotypes sampled from different areas (Samsun, Trabzon, Ordu, and Giresun). Variation was determined regarding morphological characteristics, and that plant shape, flower, and leaf properties were highly variable. Molecular data support morphological features. Thirteen ISSR primers determined the dimensions of genetic diversity, and the genotypes’ similarity indexes were between 0.70 and 0.92. It is seen that there is no clustering in the two- and three-dimensional graphics created in line with the genetic parameters, and the genotypes are distributed. Also, plant characteristics were correlated with the ISSR data, and many DNA profiles were detected. This study has characterized Primula genotypes that naturally spread in the Black Sea region of Türkiye. It is a guiding study for transferring some observed properties to culture varieties.

show abstract

Effect of plant growth regulators and explant types on induction and growth of callus of Primula veris L.

Cited by 3 publications

References 14 publications

The Influence of Plant Growth Regulators and Light Supply on Bitter Cassava Callus Initiation for Starch Production

The Influence of Plant Growth Regulators and Light Supply on Bitter Cassava Callus Initiation for Starch Production

Ex-Situ Conservation of An Endangered Medicinal Plant Andrographis Paniculata By Plant Tissue Culture

Comprehensive assessment of genetic variation in native heterostylous primrose genotypes of Türkiye

Contact Info

Product

Resources

About