Effect of plant growth regulators on ovary culture of coconut (Cocos nucifera L.)

Perera, P. I. P.; Vidhanaarachchi, V.R.M.; Gunathilake, Tharuka; Yakandawala, Deepthi; Hocher, Valérie; Verdeil, Jean‐Luc; Weerakoon, L.K.

doi:10.1007/s11240-009-9577-z

Cited by 28 publications

(14 citation statements)

References 43 publications

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“…A high auxin concentration in association with activated charcoal has been widely used in the induction of somatic embryogenesis of palm species (Pérez-Nuñez et al 2006;Saénz et al 2006;Steinmacher et al 2007b;Othmani et al 2009;Perera et al 2009;Al-Khayri 2010). For A. aculeata, Moura et al (2009) showed that activated charcoal was essential for avoiding tissue browning and stimulating somatic embryo formation.…”

Section: Discussionmentioning

confidence: 99%

Somatic embryogenesis in Acrocomia aculeata Jacq. (Lodd.) ex Mart using the thin cell layer technique

et al. 2015

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Considering the necessity of Acrocomia aculeata propagation for large-scale production, the aim of this study was to establish a somatic embryogenesis protocol using the thin cell layer (TCL) technique. Aerial parts of in vitro plants were transversally cut at the base into eight TCLs and placed in a culture medium for callus induction. The induction medium was composed of Y3 salts and Morel´s vitamins and supplemented with 150, 300 or 600 μM picloram. After 12 weeks the calli were transferred to a medium supplemented with BAP or 2-iP (12.5 or 25 μM). After 18 weeks, the somatic embryo clusters were transferred to a conversion medium (plant growth regulator-free medium). Primary callus induction rate was higher in the first three TCLs and in media containing 150 or 300 μM picloram. The best maturation results were obtained in medium containing 12.5 μM 2-iP or 12.5 μM BAP. Few somatic embryos converted into plants. The histological analyses showed that callus induction started adjacent to vascular bundles after two days of culture, and somatic embryos arose in the periphery of nodular calli. This study showed that the TCL embryogenesis protocol is promising for in vitro multiplication of A. aculeata.

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Section: Discussionmentioning

confidence: 99%

Somatic embryogenesis in Acrocomia aculeata Jacq. (Lodd.) ex Mart using the thin cell layer technique

et al. 2015

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“…According to Garcia and co-workers (2007), light can modulate the action of different growth regulators inducing various morphogenic responses. ABA has been found to adversely affect shoot regeneration in Cocos nucifera (Perera et al 2009). This hormone also inhibited regeneration of Medicago sativa somatic embryos (Kępczyńska and Zielińska 2006).…”

Section: Discussionmentioning

confidence: 99%

Endogenous ABA content in relation to maturation of somatic embryos in Tulipa (L.) ‘Apeldoorn’ cultures

2016

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The aim of the present study was to estimate the endogenous abscisic acid (ABA) content in tulip 'Apel-doorn' torpedo and mature somatic embryos. Moreover, the effect of exogenous ABA and/or its inhibitor fluridone on somatic embryo maturation and conversion into plantlets was investigated. Torpedo-stage somatic embryos were subcultured on media containing 5 lM of picloram and 1 lM of 6-benzyl-aminopurine (BAP)-control, and combinations of ABA (0 or 10 lM) and/or fluridone (0 or 30 lM) for 1 week. Then, the torpedo embryos were transferred to a maturation medium containing 0.25 lM of a-naphthaleneacetic acid (NAA) and 2.5 lM of BAP, without ABA and fluridone treatment, and cultivated under darkness or light for ten weeks. Endogenous ABA content (first time measured in tulip somatic embryos) was evaluated by ELISA test. The obtained results revealed that the highest level of endogenous ABA, at 17.45 nmol g-1 dry weight (DW), was recorded in torpedo-stage of tulip embryo development, only after 1 week of ABA treatment, and was nearly 10 times higher in comparison with the control. Simultaneous addition of ABA and fluridone to the medium resulted in the lowering of the ABA concentration to 9.58 nmol g-1 DW. During ten weeks of maturation of the embryos, the endogenous ABA content in mature tissue of tulip somatic embryo considerably decreased to an amount 0.87-1.33 nmol g-1 DW (irrespective of ABA and fluridone treatment) and did not differ significantly from control (0.59 nmol g-1 DW). Exogenous ABA and fluridone significantly decreased the growth value of fresh weight (FW) of the tulip torpedo-shaped and mature embryos under light conditions. Percentage of the DW of the torpedo embryos treated with exogenous ABA was significantly higher (15.43-17.02) in comparison with the control (10.87). Three to three and a half times more malformed mature embryos were noted under light conditions than in darkness, irrespective of ABA and fluridone treatment. The highest percentage of mature embryos forming shoots (conversion) was observed under light conditions in the control and after fluridone treatment (26 and 20%, respectively).

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“…It occurs in the palm family (Arecaceae) and is the sole species of the genus Cocos within the subfamily Arecoideae which also contains other economically important species including date palm and oil palm [4]. Prasanthi Perera, et al [6] add that it is a cross-pollinating palm, propagated only by seeds, whilst tissue culture is the only vegetative propagation method available for coconut. According to the description of the coconut tree, it has many names based on its taxonomic hierarchy as shown below (Table 1).…”

Section: Characteristics Of Coconut Plants In Tropical Coastal Regionsmentioning

confidence: 99%

The role of coconut plants in relation to disaster management in the tropical coastal regions

Rajendra

Sumariati

2018

MATEC Web Conf.

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This paper investigates the role of coconut plants growing in the tropical coastal regions in relation to disaster management. The presence of coconut trees on the beach is not solely as a part of landscape architecture but it can also be used as an option in emergency rescue in the occurrence of disaster or tsunami. For this purpose, the investigation was carried out through literature studies and documentation in data collection methods. Video documentation on the attacks of tsunami taking place in some parts of the world were important sources in collecting data. The results of the data collection were then analyzed into qualitative descriptive for verifying the roles of coconut plants in the emergency rescue. Although their roles may not be effective for children, elder and disable people, they are very useful for protection along the coast. The installation of the trees must not only be in public areas but also in private areas along the coast. Since the improvement of disaster management has to cover safety in all aspects, the result of this paper, therefore, may be useful for coastal planning and design guidelines.

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Effect of plant growth regulators on ovary culture of coconut (Cocos nucifera L.)

Cited by 28 publications

References 43 publications

Somatic embryogenesis in Acrocomia aculeata Jacq. (Lodd.) ex Mart using the thin cell layer technique

Somatic embryogenesis in Acrocomia aculeata Jacq. (Lodd.) ex Mart using the thin cell layer technique

Endogenous ABA content in relation to maturation of somatic embryos in Tulipa (L.) ‘Apeldoorn’ cultures

The role of coconut plants in relation to disaster management in the tropical coastal regions

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