Effect of Plant Hormone Gibberellic Acid-3 on Rat Testicular Biomarkers and Its Histology

Suriamurthy, Muthu

doi:10.20959/wjpps20179-10021

Cited by 4 publications

(10 citation statements)

References 45 publications

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“…Suriamurthy and Srikumar [42] registered too differential response to the gibberellic acid (GA3) doses offered to rats. Levels of cholesterol, triglyceride, protein, albumin, urea, uric acid and creatinine were augmented with that of malondialdehyde (MDA) and glutathione peroxidise (GPx) at all doses of GA3 used.…”

Section: Discussionmentioning

confidence: 99%

Dietary polyethylene inclusion affecting rabbit's performance

Abdelhamid

Hamid

Hassan³

et al. 2021

J. Appl. Sci. Eng. Technol. Educ.

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A feeding experiment was conducted with rabbits as experimental animals for 2 months at 4 treatment groups under the same environmental conditions. The aim was to study the toxic effects of dietary graded levels of polyethylene (PE, 0, 1, 2, and 4 g/ton diet). The main effects were that some animals fed the PE-contaminated diets suffered from mange, diarrhea, weakness, and/or loss of weight. Post-mortem examination of the contaminated-diets fed rabbit revealed presence of bad smell liquids in its abdomen, residues of collective PE in the small intestine, collective feces in one part of the intestine and the parts were filled with aqueous accumulation, the color of one hepatic lobule was pale, and the lungs' color was bloody red. Yet, feed intake and water consumption increased by feeding the contaminated diets. Moreover, animals offered the contaminated diets reflected heavier body weight. However, the feed conversion (utilization) was negatively affected by the PE- contaminated diet; since, dietary PE-inclusion increased apparently feed consumption than the real body weight gain. Slaughter test of the PE-treated animals reflected many remarks including loss of rabbit's weight; increased skin weight, skin was thick and dough, changes in the color and quality of the carcass flesh, easy broken bone. When these animals were eviscerated, it were observed white-colored lungs, with necrosis and bloody infiltration, small-sized liver, with blackly-red color, enlarged gall bladder, narrow diameter of the small intestine, presence of collective mass of PE on the internal wall of the small intestine. The presence of PE in the experimental diets was responsible for significant lowering the specific gravity of the treated animals' bone, as well as their flesh moisture and fat; yet, their crude protein increased besides their edible parts and boneless meat percentages increased too, but not significantly. Dietary contamination with PE reflected its residues in the rabbits' flesh and increased the flesh content of ash (P>0.05). The contaminated diets were responsible for significantly (P≤0.05) evaluating lymphocytes %, MCV, MCH, and PLT and lowering both monocytes and granulocytes % as well as RBCs count. Significant (P≤0.05) increases in albumin, creatinine and uric acid concentrations and decreases of ALT activity were recorded with feeding rabbits the PE-contaminated diets; but, Cho. and Trig. concentrations were decreased (P≤0.05). There was a significant increase of scores of hepatic congestion and necrosis in liver from rabbits group received 0.4 PE when compared with control group. Statistical analysis shows significant increase of scores of renal congestion, fibrosis and acute tubular necrosis in group received 0.4 PE when compared with control group. Hence PE negatively affected rabbits' performance, health and quality, and may affect the consumers' health too.

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Section: Discussionmentioning

confidence: 99%

Dietary polyethylene inclusion affecting rabbit's performance

Abdelhamid

Hamid

Hassan³

et al. 2021

J. Appl. Sci. Eng. Technol. Educ.

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“…Our findings are in accordance with several studies that proved the histopathological alterations induced by gibberellic acid. GA resulted in damage of testicular cells, reduced testicular weight, induced a disturbance in the testicular architecture, disorganization of spermatogenic cells, widening of intercellular spaces as well as intertubular spaces, focal separation of germinal epithelium from the basement membrane, cytoplasmic vacuolation, pyknosis also exfoliation of spermatogenic cells into the tubules lumen [5,15,30] . Some studies demonstrated that GA caused morphometric alterations in testicles and disorders in spermatogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…GA caused a reduction in the size of seminiferous tubules, a significant decrease of both germinal epithelium height and the diameter of seminiferous tubules. Concerning spermatogenesis, GA induced hypospermatogenesis and a reduction in all stages of spermatogenesis, as well as a significant decrease in sperm count in the lumen of seminiferous tubules and epididymal sperm viability [1,5,30,31] Previous studies have reported that testicular toxicity induces alterations in morphometric assessments and spermatogenesis. A significant reduction in the diameter and germ cell height of seminiferous tubules was recorded in testes of rats treated with tacrolimus when compared with the control rats [32] .…”

Section: Discussionmentioning

confidence: 99%

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Ameliorating Impact of Phoenix dactylifera L. Leaves Oil Extract on Testicular Toxicity Induced by Gibberellic Acid: Histomorphometric and Immunohistochemical Studies

Ahmed

Nofal²

2020

Egyptian Journal of Histology

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Background: Gibberellic acid is an endogenous plant growth regulator, it has many toxic effects including testicular toxicity. Phoenix dactylifera L. (date palm) is widely used in traditional medicine as anticancer, hepatoprotective, neuroprotective, nephroprotective, gastrointestinal protective and antimicrobial agent. Aim: The present work aimed to clarify the impact of Phoenix dactylifera L. leave oil extract on testicular toxicity induced by gibberellic acid in albino rats. Materials and Methods: Five groups of rats were used. Group1: control, Group 2: corn oil group, Group 3: Phoenix dactylifera L. leaves oil extract group , Group 4: Gibberellic acid group .Group 5 : Phoenix dactylifera L. leaves oil extract and Gibberellic acid group. Histomorphometric, Histochemical, immunohistochemical and biochemical alterations were investigated in testicular tissue and serum. Results: Gibberellic acid induced histopathological alterations, significantly decreased the Johnsen score of spermatogenesis, and significantly decreased the germ cell proliferation index (PI). , significantly decrease the epithelial height and tubular diameter and total glycogen content. Immuohistochemically, Gibberellic acid significantly decreased PCNA and GULT-3, while α-SMA was significantly increased. Gibberellic acid decreased serum testosterone, progesterone and LH hormones and testicular antioxidants, and significantly increased testicular MDA. Treating rats with both Phoenix dactylifera L. leaves oil extract and Gibberellic acid ameliorated all histopathological morphometric, histochemical, immunohistochemical and biochemical alterations induced by Gibberellic acid. Conclusion: Phoenix dactylifera L. leaves oil extract could ameliorate the histopathological and toxicological alterations induced by Gibberellic acid.

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“…A long-term high dose administration of GA3 has been shown to cause increased testicular weight and decreased sperm count in male albino rats (16). Another study found markedly reduced aspartate transaminase (AST) and alanine transaminase (ALT) enzyme activities in the testes of rats exposed to GA3, which was explained by the possible toxic effect of GA3 on the testes, resulting in injury to testicular cells (17). A study with neonatal rats The change in IL-10 levels in the subchronic treatment group.…”

Section: Discussionmentioning

confidence: 99%

Effect of Gibberellic Acid on the Levels of Tumor Necrosis Factor-α, Interleukin-4 and Interleukin-10 in Rats

Öztürk¹,

Erin²,

Balcı³

2019

Turk J Endocrinol Metab

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Objective: Gibberellic acid is a plant growth factor. Several studies have demonstrated the effect of gibberellic acid on tumor development. Tumor necrosis factor-α, interleukin-4 and interleukin-10 are important cytokines involved in the regulation or prevention of cancer. The aim of this study was to investigate whether changes in cytokine balance have any role in the tumor-inducing effect of gibberellic acid. Material and Methods: In order to examine the acute and subchronic effect of gibberellic acid, 50 male Wistar rats were divided into five groups. For the subchronic treatments, the first group received 2 mg/kg gibberellic acid, the second group received 20 mg/kg gibberellic acid, and the third group received only the solvent via intraperitoneal injections given three times a week for a 30 days. In the acute treatment group, a single does of either 20 mg/kg gibberellic acid or the solvent alone was administered via intraperitoneal injection, and the tissues were dissected three hours after the injection. Results: During the study period (30 days), both doses of gibberellic acid resulted in a decrease in Tumor necrosis factor-α levels, and a dose-dependent decrease in interleukin-4 levels was observed. However, no change was observed in interleukin-10 levels. Conclusion: We conclude that gibberellic acid influenced the cytokine balance. The resulting changes may be responsible for the development or advancement of inflammatory diseases and malignancies.

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Effect of Plant Hormone Gibberellic Acid-3 on Rat Testicular Biomarkers and Its Histology

Abstract: ABSTRACT

Cited by 4 publications

References 45 publications

Dietary polyethylene inclusion affecting rabbit's performance

Dietary polyethylene inclusion affecting rabbit's performance

Ameliorating Impact of Phoenix dactylifera L. Leaves Oil Extract on Testicular Toxicity Induced by Gibberellic Acid: Histomorphometric and Immunohistochemical Studies

Effect of Gibberellic Acid on the Levels of Tumor Necrosis Factor-α, Interleukin-4 and Interleukin-10 in Rats

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