Effect of Plant Lectins on the Larval Development of European Corn Borer (Lepidoptera: Pyralidae) and Southern Corn Rootworm (Coleoptera: Chrysomelidae)

Czapla, T.H.; Lang, Bruce A.

doi:10.1093/jee/83.6.2480

Cited by 168 publications

(97 citation statements)

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“…The European corn borer (Ostrinia nubilalis) and the Southern corn rootworm (Diabrotica undecrmpunctata) both feed on maize plants (Czapla and Lang, 1990). WGA and the Baukinia purpurea seed lectin were lethal to neonate Ostrinia nubilalis larvae at fairly low concentrations.…”

Section: The Anti-insect Actlvlty Of Plant Lectinsmentioning

confidence: 99%

Lectins as Plant Defense Proteins

Peumans¹,

Damme²

1995

Plant Physiol.

1,040

597

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Section: The Anti-insect Actlvlty Of Plant Lectinsmentioning

confidence: 99%

Lectins as Plant Defense Proteins

Peumans¹,

Damme²

1995

Plant Physiol.

1,040

597

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“…Based on glycan-binding specificity, JRLs are divided into two groups: Galspecific and Man-specific agglutinins (Peumans et al, 2001). Jacalin has been shown to exhibit insecticidal activity against an important pest of maize (Zea mays), the Southern corn rootworm (Diabrotica undecimpunctata; Czapala and Lang, 1990), thus suggesting the role of plant defense for JRLs. In continuation of our previous work on the molecular characterization of wheat Hfr-1 (Subramanyam et al, 2006), we investigated the function of HFR1 protein.…”

mentioning

confidence: 99%

Functional Characterization of HFR1, a High-MannoseN-Glycan-Specific Wheat Lectin Induced by Hessian Fly Larvae

et al. 2008

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We previously cloned and characterized a novel jacalin-like lectin gene from wheat (Triticum aestivum) plants that responds to infestation by Hessian fly (Mayetiola destructor) larvae, a major dipteran pest of this crop. The infested resistant plants accumulated higher levels of Hfr-1 (for Hessian fly-responsive gene 1) transcripts compared with uninfested or susceptible plants. Here, we characterize the soluble and active recombinant His 6 -HFR1 protein isolated from Escherichia coli. Functional characterization of the protein using hemagglutination assays revealed lectin activity. Glycan microarray-binding assays indicated strong affinity of His 6 -HFR1 to Mana1-6(Mana1-3)Man trisaccharide structures. Resistant wheat plants accumulated high levels of HFR1 at the larval feeding sites, as revealed by immunodetection, but the avirulent larvae were deterred from feeding and consumed only small amounts of the lectin. Behavioral studies revealed that avirulent Hessian fly larvae on resistant plants exhibited prolonged searching and writhing behaviors as they unsuccessfully attempted to establish feeding sites. During His 6 -HFR1 feeding bioassays, Drosophila melanogaster larvae experienced significant delays in growth and pupation, while percentage mortality increased with progressively higher concentrations of His 6 -HFR1 in the diet. Thus, HFR1 is an antinutrient to dipteran larvae and may play a significant role in deterring Hessian fly larvae from feeding on resistant wheat plants.

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“…Lectins from snowdrop, pea, wheat, rice, castor, soybean, mungbean, garlic, sweet potato, tobacco, chickpea and groundnut have been isolated and characterised. Lectins produce chronic effects on survival and development of insect pests elonging to different insect orders (Shukle and Murdock, 1983;Czapla and Lang, 1990;Habibi et al 1992;Powell et al 1995). Transgenic tobacco expressing pea lectin has shown adverse effects against H. virescens (Boulter et al 1990).…”

Section: Plant Lectinsmentioning

confidence: 99%

Prospects for using transgenic resistance to insects in crop improvement

Sharma¹,

Sharma²,

Seetharama³

et al. 2000

Electron. J. Biotechnol.

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Integrated pest management has historically placed great hopes on host plant resistance. However, conventional host-plant resistance to insects involves quantitative traits at several loci. As a result, the progress has been slow and difficult to achieve. With the advent of genetic transformation techniques, it has become possible to clone and insert genes into the crop plants to confer resistance to insect pests. Resistance to insects has been demonstrated in transgenic plants expressing genes for δ δ-endotoxins from Bacillus thuringiensis (Bt), protease inhibitors, enzymes and plant lectins. Most of the plant derived genes produce chronic rather than toxic effects and some insect pests are not sensitive to some of these factors. The potential of plant derived genes can be realised by deploying them in combination with host plant resistance and exotic genes. Genes conferring resistance to insects have been inserted into crop plants such as maize, cotton, potato, tobacco, potatoes, rice, broccoli, lettuce, walnuts, apples, alfalfa and soybean. Genetically transformed crops with Bt genes have been deployed for cultivation in USA, China and Australia. However, very little has been done to use this technology for improving crop production in the harsh environments of the tropics, where the need for increasing food production is most urgent. International agricultural research centres, advanced research institutes and the seed sector should make an effort to use these new tools for increasing food *Corresponding author production in poorer regions of the world. There is an urgent need to develop a scientifically sound strategy to deploy exotic and plant derived genes for minimising the extent of losses caused by insect pests. Equally important is the need for following the biosafety regulations, more responsible public debate, social attitude and better presentation of the benefits for a rational deployment of the genetically transformed plants.

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Effect of Plant Lectins on the Larval Development of European Corn Borer (Lepidoptera: Pyralidae) and Southern Corn Rootworm (Coleoptera: Chrysomelidae)

Cited by 168 publications

References 0 publications

Lectins as Plant Defense Proteins

Lectins as Plant Defense Proteins

Functional Characterization of HFR1, a High-MannoseN-Glycan-Specific Wheat Lectin Induced by Hessian Fly Larvae

Prospects for using transgenic resistance to insects in crop improvement

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