Effect of plasma components on the stability and permeability of microcapsule

Chen, Li; Zhang, Ying; Shen, Li; Wang, Xiuli; Li, Na; Wang, Yu; Guo, Xin; Zhao, Shan; Wu, Yu; Sun, Guangwei; Liu, Yang; Ma, Xiaojun

doi:10.1002/jbm.a.34907

Cited by 7 publications

(3 citation statements)

References 37 publications

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Section: Discussionmentioning

confidence: 99%

“…Nonetheless, little attention has been paid to the osmolarity adjusting agents as a tool to regulate the final outcome of the therapy. Considering that microcapsule formation occurs by electrostatic interactions, this process is directly influenced by the presence of electrolytes, especially divalent cations, in the surrounding media (Chen et al., 2014 ; Thu et al., 1996 ). Hence, we hypothesized that in the attempt to design solutions that meet the standards for cell culture, the choice of different types of osmolarity adjusting agents may play a pivotal role in the mechanics of the capsule.…”

Section: Discussionmentioning

confidence: 99%

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The role of osmolarity adjusting agents in the regulation of encapsulated cell behavior to provide a safer and more predictable delivery of therapeutics

et al. 2017

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Transplantation of cells within alginate microspheres has been extensively studied for sustained drug delivery. However, the lack of control over cell behavior represents a major concern regarding the efficacy and the safety of the therapy. Here, we demonstrated that when formulating the biosystem, an adequate selection of osmolarity adjusting agents significantly contributes to the regulation of cell responses. Our data showed that these agents interact in the capsule formation process, influencing the alginate crosslinking degree. Therefore, when selecting inert or electrolyte-based osmolarity adjusting agents to encapsulate D1 multipotent mesenchymal stromal cells (MSCs), alginate microcapsules with differing mechanical properties were obtained. Since mechanical forces acting on cells influence their behavior, contrasting cell responses were observed both, in vitro and in vivo. When employing mannitol as an inert osmolarity adjusting agent, microcapsules presented a more permissive matrix, allowing a tumoral-like behavior. This resulted in the formation of enormous cell-aggregates that presented necrotic cores and protruding peripheral cells, rendering the therapy unpredictable, dysfunctional, and unsafe. Conversely, the use of electrolyte osmolarity adjusting agents, including calcium or sodium, provided the capsule with a suitable crosslinking degree that established a tight control over cell proliferation and enabled an adequate therapeutic regimen in vivo. The crucial impact of these agents was confirmed when gene expression studies reported pivotal divergences not only in proliferative pathways, but also in genes involved in survival, migration, and differentiation. Altogether, our results prove osmolarity adjusting agents as an effective tool to regulate cell behavior and obtain safer and more predictable therapies.ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The role of osmolarity adjusting agents in the regulation of encapsulated cell behavior to provide a safer and more predictable delivery of therapeutics

et al. 2017

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“…Chen et al assessed the stability of various alginate-based microcapsules in plasma, finding that alginate-a-poly (L-lysine)alginate (a-APA) microcapsules demonstrated superior stability over alginate-ϵ-poly (L-lysine)-alginate (ϵ-APA) and alginatechitosan-alginate (ACA) capsules. The stability of these capsules was influenced by different factors, with heparin significantly affecting a-APA microcapsules, while HCO3-and H2PO4-/ HPO42-impacted ϵ-APA and ACA capsules, respectively (67). Liu introduced a method for creating porous alginate beads (PABs) using an aqueous two-phase system (ATPS) emulsion technique, blending a cell/dextran (Dex) mixture with an alginate (Alg)/ polyethylene glycol (PEG) mixture.…”

Section: Future Directions In Hepatocyte Encapsulationmentioning

confidence: 99%

A comprehensive review of advances in hepatocyte microencapsulation: selecting materials and preserving cell viability

Wang,

Wen,

Jiang

et al. 2024

Front. Immunol.

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Liver failure represents a critical medical condition with a traditionally grim prognosis, where treatment options have been notably limited. Historically, liver transplantation has stood as the sole definitive cure, yet the stark disparity between the limited availability of liver donations and the high demand for such organs has significantly hampered its feasibility. This discrepancy has necessitated the exploration of hepatocyte transplantation as a temporary, supportive intervention. In light of this, our review delves into the burgeoning field of hepatocyte transplantation, with a focus on the latest advancements in maintaining hepatocyte function, co-microencapsulation techniques, xenogeneic hepatocyte transplantation, and the selection of materials for microencapsulation. Our examination of hepatocyte microencapsulation research highlights that, to date, most studies have been conducted in vitro or using liver failure mouse models, with a notable paucity of experiments on larger mammals. The functionality of microencapsulated hepatocytes is primarily inferred through indirect measures such as urea and albumin production and the rate of ammonia clearance. Furthermore, research on the mechanisms underlying hepatocyte co-microencapsulation remains limited, and the practicality of xenogeneic hepatocyte transplantation requires further validation. The potential of hepatocyte microencapsulation extends beyond the current scope of application, suggesting a promising horizon for liver failure treatment modalities. Innovations in encapsulation materials and techniques aim to enhance cell viability and function, indicating a need for comprehensive studies that bridge the gap between small-scale laboratory success and clinical applicability. Moreover, the integration of bioengineering and regenerative medicine offers novel pathways to refine hepatocyte transplantation, potentially overcoming the challenges of immune rejection and ensuring the long-term functionality of transplanted cells. In conclusion, while hepatocyte microencapsulation and transplantation herald a new era in liver failure therapy, significant strides must be made to translate these experimental approaches into viable clinical solutions. Future research should aim to expand the experimental models to include larger mammals, thereby providing a clearer understanding of the clinical potential of these therapies. Additionally, a deeper exploration into the mechanisms of cell survival and function within microcapsules, alongside the development of innovative encapsulation materials, will be critical in advancing the field and offering new hope to patients with liver failure.

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Improved membrane stability of alginate-chitosan microcapsules by crosslinking with tannic acid

Chen

Jiang

et al. 2023

Biotechnol Lett

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Effect of plasma components on the stability and permeability of microcapsule

Cited by 7 publications

References 37 publications

The role of osmolarity adjusting agents in the regulation of encapsulated cell behavior to provide a safer and more predictable delivery of therapeutics

The role of osmolarity adjusting agents in the regulation of encapsulated cell behavior to provide a safer and more predictable delivery of therapeutics

A comprehensive review of advances in hepatocyte microencapsulation: selecting materials and preserving cell viability

Improved membrane stability of alginate-chitosan microcapsules by crosslinking with tannic acid

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