Effect of plasma progesterone on oocyte recovery, oocyte quality, and early in-vitro developmental competence of embryos in Bos indicus dairy cows

Saad, Muhammad; Sarwar, Zaeem; Saleem, Muhammad Hamzah; Arshad, Usman; Shahzad, Muhammad; Mushtaq, Muhammad; Husnain, A.; Riaz, Amjad; Ahmad, Nasim

doi:10.1016/j.anireprosci.2019.02.001

Cited by 18 publications

(20 citation statements)

References 48 publications

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“…In all treatment protocols and all cows (n=666), the pregnancy rate of cows with >2 CLs on their ovaries at PG was higher than for cows with ≤2 CLs on their ovaries at the same time (Table 3). This result is consistent with the results of other studies that have confirmed the positive effects of high serum concentrations of P4 in the luteal phase before insemination on the pregnancy rate of cows (Rivera et al, 2011;Bisinotto et al, 2015;Saad et al, 2019).…”

Section: Discussionsupporting

confidence: 93%

“…As a result, high serum concentrations of P4 in these cows had positive effects on the quality of the preovulatory follicle, oocyte and uterine environment for embryo development. It has been shown that high serum concentrations of P4 in the preinsemination dioestrous phase increases the quality of the ovulatory follicle and oocyte, and the chance of a successful pregnancy is increased following the ovulation of a qualified follicle (Rivera et al, 2011;Bisinotto et al, 2015;Saad et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, subgroup of cows with high serum concentrations of P4 at GnRH1 (>2.8 ng/mL) showed a statistically significant difference between the pregnancy rates of cows in the PO compared to the G6G protocol (P=0.03, Table 2). Various studies have shown the beneficial effects of high serum concentrations of P4 in the pre-insemination luteal phase on the pregnancy rate of cows (Rivera et al, 2011;Bisinotto et al, 2015;Saad et al, 2019). In the current study, since the serum concentration of P4 at GnRH1 was significantly higher in cows in the G6G compared to the PO protocol, this could Comparison of the effects of Presynch-Ovsynch, Presynch-Ovsynch+CIDR, and G6G protocols on the reproductive performance of lactating Holstein cows in the postpartum period Usporedba učinaka Presynch-Ovsynch, Presynch-Ovsynch+CIDR i G6G protokola na reproduktivne pokazatelje Holstein krava u laktaciji u razdoblju nakon teljenja VETERINARSKA STANICA xx (x), x-x, xxxx.…”

Section: Discussionmentioning

confidence: 99%

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Comparison of the effects of Presynch-Ovsynch, Presynch- Ovsynch+CIDR, and G6G protocols on the reproductive performance of lactating Holstein cows in the postpartum period

Kohsari

Berenjian²

2021

Vet. stn. (Online)

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This study was performed on an industrial dairy farm near Tehran province, Iran, on 666 lactating Holstein cows from September 2019 to June 2020 in order to eliminate the harmful effects of heat stress on the reproductive performance of the cows. The hypothesis of the recent study was that by increasing the serum concentration of progesterone (P4) in the luteal phase before insemination in the form of Presynch-Ovsynch+CIDR (POC) and G6G protocols, the pregnancy rate of cows would be increased compared to the Presynch-Ovsynch (PO) protocol. The aim of the present study was to evaluate the effects of Presynch-Ovsynch (PGF2α-14d- PGF2α-12d-Ovsynch (OVS: GnRH(GnRH1)- 7d-PGF2α(PG)-2d-GnRH)-18h-Fixed-time artificial insemination (FTAI), n=212), Presynch-Ovsynch+CIDR (similar to the PO protocol, plus the use of a CIDR for 7 days, from the start of the OVS+FTAI protocol to the time of PG injection, n=230), and G6G (PGF2α- 2d-GnRH-6d-OVS+FTAI, n=224) protocols on the reproductive performance and pregnancy rate of lactating Holstein cows. The average body condition score (BCS) for the cows was about 2.5 at the time of the onset of the OVS+FTAI program. The average daily milk production for cows at the time of the start of the OVS+FTAI program was 38.3 kg/day. On average, the studied cows were inseminated 86 days postpartum. Implementation of the G6G protocol in comparison to the PO and POC protocols increased the serum concentration of P4 at GnRH1 (P=0.04). The cows that received the G6G protocol had a greater number of corpuses luteum (CL) on their ovaries at PG in comparison to the cows in the PO and POC protocols (P=0.03 and P=0.05, respectively). For all treatment protocols, the pregnancy rate of cows with >2 CLs on their ovaries at PG was significantly higher than for cows with ≤2 CLs on their ovaries at this time. The overall pregnancy rate for all cows studied was 42%. Separately, pregnancy rates for cows in PO, POC, and G6G protocols were calculated at 36.7%, 41.7%, and 47.3% respectively. The highest and the lowest pregnancy rates were obtained in the G6G and PO protocols, respectively (P=0.03). It can be concluded that the G6G protocol increased the pregnancy rate of cows in comparison to the PO but not to the POC protocol. This result can be attributed to the increase in serum concentration of P4 at GnRH1 and the greater number of CLs at PG following implementation of the G6G compared to the PO protocol. Although CIDR administration in the POC protocol led to an increase in the pregnancy rate of cows compared to the PO protocol, possibly by increasing the synchrony rate and the quality of the ovulatory follicle, this increase was not statistically significant (P=0.09).

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of the effects of Presynch-Ovsynch, Presynch- Ovsynch+CIDR, and G6G protocols on the reproductive performance of lactating Holstein cows in the postpartum period

Kohsari

Berenjian²

2021

Vet. stn. (Online)

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“…Even in the luteal phase, the follicles are sensitive to exogenous gonadotrophins that determine an increase in their size and, if the CL is lysed through prostaglandin administration, ovulation can occur as well, and the oocytes might even undergo regular fertilization. Many studies were therefore successfully carried out also in vitro and highlighted the fact that oocytes retrieved after LPS might result in good-quality blastocysts and viable offspring after assisted reproductive technology (ART) (18). Also, in the horse, it is well known that follicles respond to gonadotrophins and are selected in the presence of CL.…”

Section: A Glimpse Of Double Stimulation In Animal Modelsmentioning

confidence: 99%

DuoStim – a reproducible strategy to obtain more oocytes and competent embryos in a short time-frame aimed at fertility preservation and IVF purposes. A systematic review

Vaiarelli

Cimadomo

Petriglia

et al. 2020

Upsala Journal of Medical Sciences

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Recent evidence suggests that follicular development occurs in a wave-like model during the ovarian cycle, where up to three cohorts of follicles are recruited to complete folliculogenesis. This understanding overtakes the previous dogma stating that follicles grow only during the follicular phase of the menstrual cycle. Therefore, in in vitro fertilization (IVF), novel protocols regarding ovarian stimulation have been theorized based on the use of gonadotrophins to prompt the growth of antral follicles at any stage of the menstrual cycle. These unconventional protocols for ovarian stimulation aim at a more efficient management of poor-prognosis patients, otherwise exposed to conflicting outcomes after conventional approaches. DuoStim appears among these unconventional stimulation protocols as one of the most promising. It combines two consecutive stimulations in the follicular and luteal phases of the same ovarian cycle, aimed at increasing the number of oocytes retrieved and embryos produced in the short time-frame. This protocol has been suggested for the treatment of all conditions requiring a maximal and urgent exploitation of the ovarian reserve, such as oncological patients and poor responders at an advanced maternal age. At present, data from independent studies have outlined the consistency and reproducibility of this approach, which might also reduce the drop-out between consecutive failed IVF cycles in poor-prognosis patients. However, the protocol must be standardized, and more robust studies and cost-benefit analyses are needed to highlight the true clinical pros and cons deriving from DuoStim implementation in IVF.

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“…However, genetic exploitation through in-vitro embryo production (IVEP) in the farm animals remained challenging during the last three decades ( Thompson, 1997 ) The IVEP involves oocyte collection, maturation, sperm capacitation, fertilization, and embryo culture ( Rose and Bavister, 1992 ). Several factors influence the outcome of IVEP technique which include, oocytes obtained either from slaughtered or live animal ( Gasparrini, 2002 ), parity ( Snijders et al., 2000a ), age ( Armstrong, 2001 ), stage of the estrous cycle ( De Wit et al., 2000 ), blood progesterone concentration ( Saad et al., 2019 ), body condition score (BCS), milk production ( Snijders et al., 2000b ), season of the year ( Al-Katanani et al., 2002 ), cyclicity ( Boediono et al., 1995 ), capacitation method ( Parrish et al., 1995 ), sex preselection of embryos ( Chowdhury et al., 2019 ) and size of the follicle ( Pavlok et al., 1992 ; Lonergan et al., 1994 ). These factors are in addition to the laboratory procedures ( Gordon, 2003 ) which are the key determinants for the success of IVEP technique.…”

Section: Introductionmentioning

confidence: 99%

Effect of follicle size on oocytes recovery rate, quality, and in-vitro developmental competence in Bos indicus cows

et al. 2020

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The objective of the present study was to determine the effect of follicle size on recovery rate, quality, and in-vitro developmental competence of oocytes in Bos indicus cows. The ovaries (n = 507) of Bos indicus cows having age of 5-8 years, with mixed parity, BCS 2.75 ± 0.25, and clinically normal reproductive tracts were collected from the local abattoir. The follicles on the ovaries were divided into two groups based upon their size; 1) ≥6 mm diameter, and 2) <6 mm diameter. After initial evaluation of quality of the oocytes, the COCs were in vitro matured, fertilized, and cultured to determine the in vitro developmental competence. The oocyte recovery, quality, maturation, cleavage, 4-cell, 8-cell, and 16-cell stages were analyzed using PROC GLIMMIX procedure of SAS. However, the number of oocytes recovered per ovary was analyzed using MIXED procedure of SAS. Results revealed that the recovery of oocytes (LSM ± SEM) derived from the follicles having size <6 mm per ovary was greater (1.02 vs. 3.14 ± 0.13; P < 0. 0001). However, the percentage (n/n) recovery [69.8 (474/679) vs. 62.7% (1454/2320); P = 0.01] and grade I_+_II oocytes [68.4 (324/474) vs. 57.9% (842/1454); P < 0.0001] was greater in ≥6 mm as compared with <6 mm group, respectively. However, maturation rate did not differ [92.9 (288/310) vs. 92.2% (296/321); P = 0.98] between the groups. In contrast, cleavage rate [58.1 (180/310) vs. 47.4% (152/321); P = 0.01], the 4-cell [34.5 (107/310) vs. 18.7% (60/321); P = 0.0003], 8-cell [15.5 (48/310) vs. 7.8% (25/321); P = 0.008], and 16-cell [8.7 (27/310) vs. 2.1% (7/321); P = 0.004] stage embryos were greater in ≥6 mm group. It can be concluded that oocytes derived from follicle ≥6 mm have better in vitro developmental competence based on embryonic conversion in Bos indicus cows.

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Effect of plasma progesterone on oocyte recovery, oocyte quality, and early in-vitro developmental competence of embryos in Bos indicus dairy cows

Cited by 18 publications

References 48 publications

Comparison of the effects of Presynch-Ovsynch, Presynch- Ovsynch+CIDR, and G6G protocols on the reproductive performance of lactating Holstein cows in the postpartum period

Comparison of the effects of Presynch-Ovsynch, Presynch- Ovsynch+CIDR, and G6G protocols on the reproductive performance of lactating Holstein cows in the postpartum period

DuoStim – a reproducible strategy to obtain more oocytes and competent embryos in a short time-frame aimed at fertility preservation and IVF purposes. A systematic review

Effect of follicle size on oocytes recovery rate, quality, and in-vitro developmental competence in Bos indicus cows

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