Effect of Plasmodium inactivation in whole blood on the incidence of blood transfusion-transmitted malaria in endemic regions: the African Investigation of the Mirasol System (AIMS) randomised controlled trial

Allain, Jean‐Pierre; Owusu-Ofori, Alex; Assennato, Sonny Michael; Marschner, Susanne; Goodrich, Raymond P.; Owusu‐Ofori, Shirley

doi:10.1016/s0140-6736(16)00581-x

Cited by 115 publications

(146 citation statements)

References 13 publications

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“…16 The clinical implication of "old" RBC units is a controversial issue, because results provided by retrospective studies and randomized clinical trials are not in agreement. [18][19][20][21][22][23][24][25][26][27] Even if the full clinical impact of the storage lesion 28 and, specifically, of this multiphase metabolic decay, remains to be elucidated, it can be argued that all the metabolomics studies performed so far showed almost complete overlap of metabolic profiles of RBCs stored in different storage additives, despite interlaboratory strategies and metabolomics platforms. 13 For these reasons, we believe that an underlying signature of the RBC storage lesion exists across various media solutions and processing techniques, and that it can be translated in biomarkers that define the metabolic stage of stored RBCs.…”

Section: Introductionmentioning

confidence: 99%

Biomarkers defining the metabolic age of red blood cells during cold storage

Paglia

D’Alessandro

Rolfsson

et al. 2016

Blood

127

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• Eight extracellular biomarkers define the metabolic age of stored RBCs.• Metabolomics defines a universal signature of RBC storage lesion.Metabolomic investigations of packed red blood cells (RBCs) stored under refrigerated conditions in saline adenine glucose mannitol (SAGM) additives have revealed the presence of 3 distinct metabolic phases, occurring on days 0-10, 10-18, and after day 18 of storage. Here we used receiving operating characteristics curve analysis to identify biomarkers that can differentiate between the 3 metabolic states. We first recruited 24 donors and analyzed 308 samples coming from RBC concentrates stored in SAGM and additive solution 3. We found that 8 extracellular compounds (lactic acid, nicotinamide, 5-oxoproline, xanthine, hypoxanthine, glucose, malic acid, and adenine) form the basis for an accurate classification/regression model and are able to differentiate among the metabolic phases. This model was then validated by analyzing an additional 49 samples obtained by preparing 7 new RBC concentrates in SAGM. Despite the technical variability associated with RBC processing strategies, verification of these markers was independently confirmed in 2 separate laboratories with different analytical setups and different sample sets. The 8 compounds proposed here highly correlate with the metabolic age of packed RBCs, and can be prospectively validated as biomarkers of the RBC metabolic lesion. (Blood. 2016;128(13):e43-e50)

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Section: Introductionmentioning

confidence: 99%

Biomarkers defining the metabolic age of red blood cells during cold storage

Paglia

D’Alessandro

Rolfsson

et al. 2016

Blood

127

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“…Pathogen-reduction technology offers an attractive method to reduce transfusion-related infection risks. Several studies have shown that this technology can reduce the risk of pathogen transmission due to contaminated blood transfusions [23, 24]. …”

Section: Discussionmentioning

confidence: 99%

Mirasol pathogen reduction technology treatment of human whole blood does not induce acute lung injury in mice

et al. 2017

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In resource-limited settings and in the military theater, fresh human whole blood is commonly transfused, but infectious risks are a concern. Sophisticated molecular testing for potential infectious agents in the whole blood is often unavailable. To address this unmet need, pathogen reduction technology (PRT) has been developed, and it is an effective approach to inactivate a broad range of pathogens found in human blood. However, studies are needed to determine if it is harmful to blood cells and whether these cells could damage the transfused recipient, including the development of acute lung injury/acute respiratory distress syndrome. In this study, we used a commercial PRT system to treat human whole blood that was then transfused into immunodeficient mice, and the development of acute lung injury was determined. In a model of transfusion-related acute lung injury (TRALI), BALB/c SCID mice developed more robust lung injury when challenged with a MHC Class I monoclonal antibody compared to BALB/c wild-type and NOD/SCID mice. Transfusion of control versus Mirasol PRT-treated whole blood (25% blood volume exchange) into BALB/c SCID mice did not produce lung injury at storage day 1. However, mild lung injury at storage days 14 and 21 was observed without significant differences in lung injury measurements between Mirasol PRT-treated and control groups. The mild storage-dependent acute lung injury correlated with trends for increased levels of cell-free hemoglobin that accumulated in both the control and Mirasol PRT-treated groups. Neutrophil extracellular traps were elevated in the plasma of BALB/c SCID mice in the monoclonal antibody TRALI model, but were not different in mice that received exchange transfusions. In conclusion, exchange transfusion of human whole blood into immunodeficient mice produces mild lung injury that is storage-dependent and not related to pathogen reduction treatment.

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“…A Phase Ⅲ clinical trial for evaluating the efficacy of the Mirasol-treated system for whole blood has been completed. Allain et al demonstrated the efficacy and safety of this technology in reducing the incidence of transfusiontransmitted malaria in epidemic areas [14]. All of these studies offer possibility that we may achieve great breakthroughs of PI treatment for the whole blood and RBCs in the near future.…”

Section: Pi Treatments For Whole Blood and Rbcsmentioning

confidence: 98%

Pathogen Inactivation Plays an Irreplaceable Role in Safeguarding the Safety of Blood Transfusion

Yao¹

2016

J Antivir Antiretrovir

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Effect of Plasmodium inactivation in whole blood on the incidence of blood transfusion-transmitted malaria in endemic regions: the African Investigation of the Mirasol System (AIMS) randomised controlled trial

Cited by 115 publications

References 13 publications

Biomarkers defining the metabolic age of red blood cells during cold storage

Biomarkers defining the metabolic age of red blood cells during cold storage

Mirasol pathogen reduction technology treatment of human whole blood does not induce acute lung injury in mice

Pathogen Inactivation Plays an Irreplaceable Role in Safeguarding the Safety of Blood Transfusion

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