Effect of Platelet Lysate on the Functional and Molecular Characteristics of Mesenchymal Stem Cells Isolated from Adipose Tissue

Castegnaro, Silvia; Chieregato, Katia; Maddalena, Marta; Albiero, Elena; Visco, Carlo; Madeo, Domenico; Pegoraro, Maurizio; Rodeghiero, Francesco

doi:10.2174/157488811795495440

Cited by 37 publications

(39 citation statements)

References 37 publications

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“…However, Ferreira et al (2005) found 50% as the optimum concentration for osteoblast proliferation. On the other hand, 10% PRP was sufficient to induce a marked cell proliferation of MSC (Lucarelli et al, 2003;Castegnaro et al, 2011), derived from adipose tissue. Similarly, 2% PRP (extracted from umbilical cord blood) showed the highest proliferation rate of dental stem cells DPSCs, PDLSCs and SHED (Lucarelli et al, 2003).…”

Section: Discussionmentioning

confidence: 93%

Comparison of osteo/odontogenic differentiation of human adult dental pulp stem cells and stem cells from apical papilla in the presence of platelet lysate

Abuarqoub

Awidi

Abuharfeil

2015

Archives of Oral Biology

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Section: Discussionmentioning

confidence: 93%

Comparison of osteo/odontogenic differentiation of human adult dental pulp stem cells and stem cells from apical papilla in the presence of platelet lysate

Abuarqoub

Awidi

Abuharfeil

2015

Archives of Oral Biology

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“…However, Ferreira et al (25) found that 50% PRP was the optimum concentration for osteoblast proliferation. On the other hand, 10% PRP was sufficient to induce a marked cell proliferation of MSC, derived from adipose tissue (28). In addition, 5% PL considered as the optimum concentration for MSC and DPSCs proliferation and osteogenesis (29).…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Human Platelet Rich Fibrin Exudate vs Fetal Bovine Serum on Proliferation and Differentiation of Dental Pulp Stem Cells

Saeed

Elrahman

Helal

et al. 2017

IJSC

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Background and ObjectivesThe imperative role of dental pulp stem cells (DPSCs) in regenerative therapy demands an in-vitro expansion which must deal with the safety and ethical problems associated with fetal bovine serum (FBS). The primary aim of this study was to compare the effects of human platelet rich fibrin (hPRF) exudate Vs FBS on proliferation and osteodifferentiation of human dental pulp stem cells (hDPSCs). The secondary one was to determine the optimum concentration of hPRF exudate inducing hDPSCs proliferation and osteodifferentiation.MethodsThe direct method was used to prepare hPRF exudate. hDPSCs were isolated from impacted mandibular third molars of twelve donors by the outgrowth method. For cell viability and proliferation rate testing, 96 well plates were used and the assay was done in duplicate and the trial repeated four times under the same conditions. Six wells were used to contain 10% FBS, serum free media, 1%, 5%, 10% and 20% concentrations of hPRF exudates, respectively. The proliferation assay was carried out by MTS tetrazolium cell proliferation assay kit and Elisa reader. The study design for osteodifferentiation protocol was exactly as the proliferation one and instead the assay was carried out by alizarin red with Elisa reader.ResultsCompared to 10% FBS, 10% hPRF exudate was the optimum concentration for hDPSCs proliferation, while 1% hPRF exudate was the optimum concentration for osteodifferentiation of hDPSCs.ConclusionsAvoiding the risk of zoonosis which may be occurred with FBS, it is recommended to use 10% hPRF exudate for proliferation and 1% for osteodifferentiation.

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“…PL can be produced from a pool of donors which allows in advance production of a GMP-approved product without extensive testing (Ploderl et al 2010; Shih et al 2010; Prins et al 2009). Several characteristics of human PL-cultured ASC have been investigated previously, indicating enhanced proliferation rate and a similar cell surface marker profile (Blande et al 2009; Kakudo et al 2008; Kocaoemer et al 2007; Bieback et al 2010; Crespo-Diaz et al 2011; Shih et al 2010; Castegnaro et al 2011). Before translating PL culturing to clinical use, more characteristics of PL-cultured ASC need to be analyzed.…”

Section: Introductionmentioning

confidence: 86%

Human platelet lysate as a fetal bovine serum substitute improves human adipose-derived stromal cell culture for future cardiac repair applications

et al. 2012

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Adipose-derived stromal cells (ASC) are promising candidates for cell therapy, for example to treat myocardial infarction. Commonly, fetal bovine serum (FBS) is used in ASC culturing. However, FBS has several disadvantages. Its effects differ between batches and, when applied clinically, transmission of pathogens and antibody development against FBS are possible. In this study, we investigated whether FBS can be substituted by human platelet lysate (PL) in ASC culture, without affecting functional capacities particularly important for cardiac repair application of ASC. We found that PL-cultured ASC had a significant 3-fold increased proliferation rate and a significantly higher attachment to tissue culture plastic as well as to endothelial cells compared with FBS-cultured ASC. PL-cultured ASC remained a significant 25% smaller than FBS-cultured ASC. Both showed a comparable surface marker profile, with the exception of significantly higher levels of CD73, CD90, and CD166 on PL-cultured ASC. PL-cultured ASC showed a significantly higher migration rate compared with FBS-cultured ASC in a transwell assay. Finally, FBS- and PL-cultured ASC had a similar high capacity to differentiate towards cardiomyocytes. In conclusion, this study showed that culturing ASC is more favorable in PL-supplemented medium compared with FBS-supplemented medium.

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Effect of Platelet Lysate on the Functional and Molecular Characteristics of Mesenchymal Stem Cells Isolated from Adipose Tissue

Abstract: hPL represents an effective and safe supplement for MSC expansion to be used in the clinical setting.

Cited by 37 publications

References 37 publications

Comparison of osteo/odontogenic differentiation of human adult dental pulp stem cells and stem cells from apical papilla in the presence of platelet lysate

Comparison of osteo/odontogenic differentiation of human adult dental pulp stem cells and stem cells from apical papilla in the presence of platelet lysate

Efficacy of Human Platelet Rich Fibrin Exudate vs Fetal Bovine Serum on Proliferation and Differentiation of Dental Pulp Stem Cells

Human platelet lysate as a fetal bovine serum substitute improves human adipose-derived stromal cell culture for future cardiac repair applications

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