Effect of polyamines on in vitro anther culture of Citrus clementina Hort. ex Tan

Abstract

The improvement of the induction rate in Citrus anther culture is important for taking practical advantage of the haploid potential in breeding. The influence of polyamines on anther culture of Citrus clementina, cv Nules, with particular attention to the free, soluble and insoluble-conjugated polyamine levels, has been investigated. Putrescine, spermidine and putrescine plus spermidine, were added to the standard induction medium. Before culture, spermidine was the most abundant among the free polyamines detected in anthers. The exogenous supply of either putrescine or spermidine, either independently or combined, effected greater uptake and accumulation of polyamines. The addition of 2 mM spermidine to the medium stimulated gametic embryogenesis in clementine Nules, whereas putrescine did not influence embryo production. Regenerants were mostly tri-haploids; a few doubled-haploids and no haploid plants were obtained. © 2006 Springer Science+Business Media B.V

Effect of polyamines on in vitro anther cultures of carrot (Daucus carota L.).

“…However, putrescine was more efficient than spermidine and spermine in increasing callus induction in green plant regeneration in rice anther cultures of Taiper 309. The best concentration to increase green plant regeneration was 10 -3 M. Chiancone et al (2006), in their studies of androgenesis in clementines using putrescine, spermidine, and a mixture of both, achieved an improvement in the efficiency of androgenesis under the influence of spermidine. By contrast, putrescine did not positively affect the formation of embryos in Citrus clementina.…”

“…An improvement in the efficiency of gynogenesis in onion has been achieved through the exogenous application of polyamines (Martinez et al, 2000;Geoffriau et al, 2006). The role of polyamines in androgenesis has been investigated by Tiainen (1992) in potatoes, Dewi et al (2004) and Dewi and Purwoko (2008) in rice, Kumar et al (2004) in cucumbers, Hema et al (2008) in niger (Guizotia abisinica L.), and Chiancone et al (2006) in clementines.…”

Antioxidant response of Nicotiana tabacum cv. Bright Yellow 2 cells to cadmium and nickel stress

“…Polyamines, which are involved in the control of numerous cellular functions, including free radical scavenging and antioxidant activity, have been found to confer protection from abiotic stresses Values are the means of 3 replicates ±SEM but their mode of action is not fully understood (Groppa and Benavides 2008). Polyamines have also been shown to play a role in cell culture induction, growth and development (Chiancone et al 2006;Santa-Catarina et al 2007;Steiner et al 2007). In sunflower, polyamines have been shown to increase dramatically in response to high Cd concentrations (1 mM) (Groppa et al 2007), which may be a mechanism of cell defense to the oxidative stress induced by Cd.…”

Androgenesis: A Fascinating Doubled Haploid Production Process

“…Various studies have indicated that 2,4-D help to enhance/promote callus growth while NAA and IAA supports direct embryogenesis (Liang et al 1987 ). The supplementation of medium with polyamines has also improved the frequency of embryos in clementine (Chiancone et al 2006 ), Cucumis sativus (Kumar et al 2004 ), and wheat (Rajyalakshmi et al 1995 ). Similar fi ndings in cereal anther culture have also been stated using arabinogalactans (Letarte et al 2006 ) and ovary co-culturing (Broughton 2008 ).…”