Effect of polyethylene particles on human osteoblastic cell growth

Martı́nez, Marı́a Eugenia; Medina, Sonia; Campo, Ma Teresa del; Garcı́a, J.A.; Rodrigo, Ana Moreno; Munuera, L.

doi:10.1016/s0142-9612(97)00250-0

Cited by 38 publications

(20 citation statements)

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“…Other investigators have shown that alumina particles increased the mortality of macrophages using Trypan blue dye exclusion and measurement of LDH release in the culture supernatant. 3 In a recent study, Martinez et al 1 showed an inhibitory effect of alumina particles (<80 m in size) on osteoblastic cell proliferation by cell count. In our work, determination of elemental intracellular ion concentration appeared to be a more sensitive probe of cell integrity than Trypan blue dye exclusion, which takes into consideration only cells with irreversible damage.…”

Section: Discussionmentioning

confidence: 98%

In vitro effects of zirconia and alumina particles on human blood monocyte-derived macrophages: X-ray microanalysis and flow cytometric studies

Nkamgueu

Adnet

Bernard

et al. 2000

J. Biomed. Mater. Res.

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The cytocompatibility of two particulate bioceramics, zirconia and alumina, was studied using human blood monocytes driven to differentiate into mature macrophages with granulocyte macrophage-colony-stimulating factor. Changes in individual cell elemental composition, particularly sodium and potassium content, were assessed by X-ray microanalysis of ultrathin freeze-dried sections. Phagocytosis and respiratory burst of macrophages exposed to biomaterial for 7 days were analyzed under flow cytometry using uptake of fluorescent latex beads and 2'7'-dichlorofluorescien diacetate oxidation, respectively. Zirconia and alumina particles were found to decrease the intracellular potassium/sodium ratio (an index of cell vitality) significantly (p<.01) in 7-day-cultured macrophages compared to control cells cultured out of material. Phagocytosis of both ceramic particles by macrophages was followed by a concomitant decrease in cell phagocytic ability (27%) and a marked altered oxidative metabolism (>2 times reduced by zirconia and >5 times reduced by alumina). The present study clearly demonstrates that reduction of the phagocytic capacity of macrophages associated with altered oxidative metabolism caused by biomaterial particles is characterized by changes in intracellular elemental content. Thus, investigation of cellular homeostasis by electron probe microanalysis together with analysis of functional changes may improve estimation of biomaterial cytocompatibility.

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Section: Discussionmentioning

confidence: 98%

In vitro effects of zirconia and alumina particles on human blood monocyte-derived macrophages: X-ray microanalysis and flow cytometric studies

Nkamgueu

Adnet

Bernard

et al. 2000

J. Biomed. Mater. Res.

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“…Most studies usually used osteoblasts obtained from cell lines [8,10,[37][38][39], except some studies where osteoblasts were isolated from human bone pieces removed during orthopedic surgical treatment [40,41]. The use of osteoblasts isolated from human bone pieces, especially at the hip or knee location, represents an interesting in vitro model as it has been shown that the reaction of osteoblasts to particles may depend on the cell lines used [37].…”

Section: Article In Pressmentioning

confidence: 99%

The influence of wear particles in the expression of osteoclastogenesis factors by osteoblasts

Pioletti

Kottelat

2004

Biomaterials

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Orthopedic implant failures are often associated with peri-implant osteolysis. Particles generated from the wear process have been suspected to play an important role in this situation. Indeed, the peri-implant osteolysis could be due to the presence of particles stimulating the osteoclastogenesis process. We hypothesize then that the presence of a low particle concentration positively influences osteoblasts to produce osteoclastogenesis factors. If true, this hypothesis would then support the idea that the particles could be at the origin of the process leading to implant loosening. To check the validity of this hypothesis, we quantified in vitro the production of different genes involved in the osteoclastogenesis process using primary isolated human osteoblasts treated or not with particles. Results showed that low concentrations of particles might have a stimulating effect on osteoblasts to produce osteoclastogenesis factors as demonstrated by the increase of RANKL and CSF-1 gene expression in the particle group. r

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“…42 An earlier paper from our group reported a slight decrease in osteoblastic cell growth in the presence of 80 m ␣-alumina particles, but this decrease was less than the one observed in the presence of 80 m polyethylene particles. 16 Other papers have studied morphologic and activity changes in osteoblastic cells on alumina surfaces and demonstrated a high degree of cytocompatibility with this material. 43 These experiments have led many investigators to consider the material as bioinert, and they have recommended it as a reference material in biocompatibility studies.…”

Section: Discussionmentioning

confidence: 99%

“…16 Briefly, trabecular bone was minced into 0.3-0.5 Quartiles Q25, Q50, and Q75 indicate the percentages of each particle size in micra.…”

Section: Cell Culturementioning

confidence: 99%

“…2,[10][11][12] The biological effect of wear debris probably depends on the type of material used, as well as the shape, size, and number of particles. [13][14][15][16][17] A previous paper by our group has demonstrated that different sizes of polyethylene particles can decrease the human osteoblastic cell growth rate in primary cultures in accordance with particle size. 16 Several in vitro studies have focused on the effects of metallic or polymeric wear debris on macrophages, [18][19][20] and it has been shown that these cells release an important number of bone-resorbing agents.…”

Section: Introductionmentioning

confidence: 97%

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Effects of MA 956 superalloy and ?-alumina particles on some markers of human osteoblastic cells in primary culture

Rodrigo

Martı́nez

Martínez

et al. 2000

J. Biomed. Mater. Res.

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One of the problems associated with the modern biomaterials used in prostheses is osteolysis, which, although its exact origin is unknown, has been associated with wear particles. Osteoblasts seem to participate directly in this phenomenon. This paper investigates in vitro cellular response to the wear particles from the metal substrate and ceramic covering (alpha-alumina) of a new titanium yttrium aluminum alloy, MA 956, that has been proposed as a biomaterial because of its exceptional mechanical and electrochemical properties. The effect of different sizes (10 and 80 microm) of MA 956 and alpha-alumina particles on osteoblast function was studied in primary human bone cell cultures. Cells were harvested from trabecular bone fragments obtained during knee arthroplasty. Osteoblastic cell response to the particles was measured by assaying C-terminal type I procollagen (PICP), alkaline phosphatase, and osteocalcin secretion, with and without 1.25(OH)(2)D(3) stimulation, in the cell-conditioned medium. Both sizes of MA 956 and alpha-alumina particles decreased PICP secretion in nonstimulated osteoblastic cells, but this secretion was not affected in the cultures stimulated with 1.25(OH)(2)D(3). Only the 10 microm alpha-alumina particles inhibited alkaline phosphatase activity in 1.25(OH)(2)D(3)-stimulated and nonstimulated cultures. The rise in osteocalcin levels after 1.25(OH)(2)D(3) stimulation was lower in the presence of the 10 microm MA 956 particles than in the presence of alpha-alumina particles. Although both materials seem to have directly affected in vitro osteoblastic cell function, the increase in osteocalcin levels after 1.25(OH)(2)D(3) stimulation was lower after exposure to MA 956 particles than the increase observed after exposure to alpha-alumina particles. Therefore, it does not seem that osteocalcin stimulated bone resorption, suggesting that MA 956 would be less likely to provoke osteolysis.

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Effect of polyethylene particles on human osteoblastic cell growth

Cited by 38 publications

References 0 publications

In vitro effects of zirconia and alumina particles on human blood monocyte-derived macrophages: X-ray microanalysis and flow cytometric studies

In vitro effects of zirconia and alumina particles on human blood monocyte-derived macrophages: X-ray microanalysis and flow cytometric studies

The influence of wear particles in the expression of osteoclastogenesis factors by osteoblasts

Effects of MA 956 superalloy and ?-alumina particles on some markers of human osteoblastic cells in primary culture

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