Effect of Polylactide/Glycolide (PLGA) Membranes Loaded With Metronidazole on Periodontal Regeneration Following Guided Tissue Regeneration in Dogs

Kurtiş, Bülent; Ünsal, Berrin; Çetiner, Deniz; Gültekin, Elif; Özcan, Gönen; Çelebi, Nevin; Ocak, Ömer

doi:10.1902/jop.2002.73.7.694

Cited by 28 publications

(26 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Metronidazole (MNA), which has selective activity against anaerobic microorganisms including bacteria and protozoa [11], has been successfully used for the treatment of anaerobic bacterial infections for more than 45 years [12]. MNA-loaded poly(lactic-co-glycolic acid) membrane fabricated by solvent casting showed a significant improvement on the periodontal regeneration following GTR in dogs [13].Our purpose is to develop an anti-infective GTR/GBR membrane with sufficient localized MNA release.…”

Section: Introductionmentioning

confidence: 99%

Drug loaded homogeneous electrospun PCL/gelatin hybrid nanofiber structures for anti-infective tissue regeneration membranes

Xue

Liu³

et al. 2014

Biomaterials

330

219

View full text Add to dashboard Cite

Email address: sharell@126.com (R. Shi). AbstractInfection is the major reason for guided tissue regeneration/guided bone regeneration (GTR/GBR) membrane failure in clinical application. In this work, we developed GTR/GBR membranes with localized drug delivery function to prevent infection by electrospinning of poly(ε-caprolactone) (PCL) and gelatin blended with metronidazole (MNA). Acetic acid (HAc) was introduced to improve the miscibility of PCL and gelatin to fabricate homogeneous hybrid nanofiber membranes. The effects of the addition of HAc and the MNA content (0,1,5,10,20,30, and 40 wt.% of polymer) on the properties of the membranes were investigated. The membranes showed good mechanical properties, appropriate biodegradation rate and barrier function. The controlled and sustained release of MNA from the membranes significantly prevented the colonization of anaerobic bacteria. Cells could adhere to and proliferate on the membranes without cytotoxicity until the MNA content reached 30%.Subcutaneous implantation in rabbits for 8 months demonstrated that MNA-loaded membranes evoked a less severe inflammatory response depending on the dose of MNA than bare membranes. The biodegradation time of the membranes was appropriate for tissue regeneration. These results indicated the potential for using MNA-loaded PCL/gelatin electrospun membranes as anti-infective GTR/GBR membranes to optimize clinical application of GTR/GBR strategies.

show abstract

Section: Introductionmentioning

confidence: 99%

Drug loaded homogeneous electrospun PCL/gelatin hybrid nanofiber structures for anti-infective tissue regeneration membranes

Xue

Liu³

et al. 2014

Biomaterials

330

219

View full text Add to dashboard Cite

show abstract

“…Many biomaterials have been analyzed to promote periodontal regeneration of tissues lost by periodontal disease, but the majority of studies used acute defects (Robert & Frank 1994, Kurtis et al 2002 or non-standardized size defects (Christgau et al 2003, Garcia et al 2011. Thus, it is believed that the experimental model described in detail here will be useful for assessment in conditions similar to those of naturally occurring periodontal disease, but more objective and standardized.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a model for induction of periodontal disease in dogs

et al. 2014

View full text Add to dashboard Cite

Pesq. Vet. Bras. 34(6):562-568, junho 2014 562 RESUMO.-[Avaliação de um modelo para indução de doença periodontal em cães.] Existem vários métodos para indução de doença periodontal em modelos animais, sendo o do defeito ósseo um dos mais descritos. Este estudo objetiva avaliar esse modelo em cães, por análises clínica, radiográfica, tomográfica e histológica, fornecendo assim dados padronizados para trabalhos futuros. Doze cães foram submetidos ao protocolo de indução. Em um primeiro procedimento cirúrgico, um retalho mucoperiosteal foi produzido na face vestibular do terceiro e do quarto dentes pré--molares direitos e foi criado um defeito, expondo a furca e parte das raízes mesial e distal, com as dimensões: 5 mm corono-apical, 5 mm mesio-distal e 3 mm vestíbulo-lingual. O ligamento periodontal e o cemento foram curetados e o defeito foi então preenchido com poliéster de moldagem, que foi removido após 21 dias em um novo procedimento cirúrgico. Exames clínicos e radiográficos foram realizados após as duas cirurgias e antes da coleta dos dentes e tecidos associados para análise tomográfica e histológica. Todos os animais apresentaram exposição de furca grau II em ambos os dentes. O nível clínico de inserção aumentou após a indução. O tamanho do defeito não apresentou alteração nas medidas corono-apical e vestíbulo-lingual, enquanto o tamanho mesio-distal foi significativamente maior que o produzido. Análise radiográfica mostrou diminuição da ra- There are several methods for inducing periodontal disease in animal models, being the bone defect one of the most reported. This study aimed to evaluate this model, through clinical, radiographic, tomographic and histological analyzes, thus providing standardized data for future regenerative works. Twelve dogs were subjected to the induction protocol. In a first surgical procedure, a mucoperiosteal flap was made on the buccal aspect of the right third and fourth premolars and a defect was produced exposing the furcation and mesial and distal roots, with dimensions: 5mm coronoapical, 5mm mesiodistal, and 3mm buccolingual. Periodontal ligament and cementum were curetted and the defect was filled with molding polyester, which was removed after 21 days on new surgical procedure. Clinical and radiographic examinations were performed after the two surgeries and before the collection of parts for dental tomography and histological analysis. All animals showed grade II furcation exposure in both teeth. Clinical attachment level increased after induction. Defect size did not change for coronoapical and buccolingual measurements, while mesiodistal size was significantly higher than at the time of defect production. Radiographic analysis showed decreased radiopacity and discontinuity of lamina dura in every tooth in the furcation area. The horizontal progression of the disease was evident in micro-computed tomography and defect content in the histological analysis. Therefore, it is concluded that this method promotes the induction of periodontal disease in dogs in a standar...

show abstract

“…These GTR membranes are usually degradable and have been modified with tetracycline, 80 doxycycline, 81 chlorhexidine 82 and metronidazole. 83 Furthermore, Bone grafting materials are utilized for bone regeneration in combination with GTR membranes. There are four classes of bone-grafting materials based upon the mode of action:…”

Section: Surgical Debridement and Bone Graftingmentioning

confidence: 99%

Peri-Implantitis: A Review of the Disease and Report of a Case Treated with Allograft to Achieve Bone Regeneration

Rashid¹,

Sheikh²,

Vohra³

et al. 2015

Dent Open J

View full text Add to dashboard Cite

Dental implants offer excellent tooth replacement options however; peri-implantitis can limit their clinical success by causing failure. Peri-implantitis is an inflammatory process around dental implants resulting in bone loss in association with bleeding and suppuration. Dental plaque is at the center of its etiology, and in addition, systemic diseases, smoking, and parafunctional habits are also implicated. The pathogenic species associated with peri-implantitis include, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Tannerella forsythia. The goal in the management of peri-implantitis is the complete resolution of peri-implant infection with function. Therapies using various biomaterials to deliver antibiotics have been used in the treatment of peri-implantitis e.g. fibers, gels, and beads. The use of guided tissue regeneration barrier membranes loaded with antimicrobials has shown success in re-osseointegrating the infected implants in animal models. Several uncertainties still remain regarding the management of peri-implantitis. The purpose of this article is to present a background of peri-implantitis along with a case of peri-implantitis successfully treated for bone regeneration.

show abstract

Effect of Polylactide/Glycolide (PLGA) Membranes Loaded With Metronidazole on Periodontal Regeneration Following Guided Tissue Regeneration in Dogs

Abstract: These results suggest that PLGA membranes with and without metronidazole may have a beneficial effect on periodontal regeneration.

Cited by 28 publications

References 30 publications

Drug loaded homogeneous electrospun PCL/gelatin hybrid nanofiber structures for anti-infective tissue regeneration membranes

Drug loaded homogeneous electrospun PCL/gelatin hybrid nanofiber structures for anti-infective tissue regeneration membranes

Evaluation of a model for induction of periodontal disease in dogs

Peri-Implantitis: A Review of the Disease and Report of a Case Treated with Allograft to Achieve Bone Regeneration

Contact Info

Product

Resources

About