Effect of polymeric adsorbents on the production of sanguinarine by Papaver somniferum cell cultures

Abstract

The suitability of adsorbent polymeric resins, Amberlite XAD-4 and XAD-7 (Rohm and Hass, Inc.), was investigated for the accumulation of sanguinarine from Papaver somniferum cell cultures. The adsorption and desorption of sanguinarine from aqueous solution was most effective with XAD-7. In addition to sanguinarine, the resins were found to absorb growth regulators and vitamins from the culture medium. Growth inhibition was overcome by delaying for approximately 4 days resin addition after cell inoculation in fresh medium. Resin addition (5% wt/vol) to actively growing uneclicited cultures led to increases in sanguinarine production and release of 30% to 40% and 60%, respectively. The addition of resins to elicited cultures led to increases in alkaloid production of up to 50% to 85% with similar increases in alkaloid release as observed for nonelicited cells. Overall yield of sanguinarine increased from 21 mg . g biomass dry weight(-1) (dw) for elicited cultures to more than 39 mg . gdw(-1) when elicitation was combined with resin addition. Higher quantities of resin (10% to 20% wt/vol) increased marginally the release of sanguinarine into the medium, and on the resin, up to 85% of total production. The use of resin appears promising for the development of a bioprocess for sanguinarine production by cultured plant cells.

In situ extraction strategy affects benzophenanthridine alkaloid production fluxes in suspension cultures ofEschscholtzia californica

“…As described by Brodelius and Pedersen (1993), transport of the secondary metabolites is redirected towards the extracellular space instead of being directed towards the vacuole, whose storage capacity limits alkaloid production. Results reported by Williams et al (1992) seem to support this hypothesis since the addition of extractive resins into cell suspensions reduced sanguinarine concentrations in both cells and medium by about 50%. However, in other cultures, the addition of an adsorptive phase did not substantially decrease cellular concentrations of alka- loids, despite increases in total production (Byun et al, 1990;Byun and Pedersen, 1994).…”

“…For example, Asada and Shuler (1989) reported a 67% increase in ajmalicine production with the addition of XAD-7 resins to Catharanthus roseus cultures, with 63% recovery on resins. Williams et al (1992) found that the addition of XAD-7 resins to Papaver somniferum cell cultures improved total sanguinarine production by 50 to 85%, with about 75% of the alkaloid recovered in the resin. A more recent report on the addition of XAD-7 to Catharanthus roseus showed that increase in production and the recovery of products were both dependant on the timing of the addition (Lee-Parsons and Shuler, 2002).…”

Critical influence of Eschscholzia californica cells nutritional state on secondary metabolite production

“…Cells were then cultured for 7 days in a production medium containing the elicitor and an extraction resin as recently described in Klvana et al (2004) (based on Archambault et al, 1996). An extractive resin was used to maximize production (Williams et al, 1992). Crude chitin (Sigma-Aldrich, Cat.…”

The effect of ajmalicine spiking and resin addition timing on the production of indole alkaloids from Catharanthus roseus cell cultures

“…In literature, the deleterious eect of resin addition at day 0 on cell viability has been attributed to the adsorption of essential hormones or vitamins from the medium (Robin and Williams et al, 1992). Williams et al (1992) reported that XAD-4 and XAD-7 resin can adsorb signi®cant levels of hormones and vitamins such as thiamin, kinetin, 2,4-dichlorophenoxyacetic acid, naphthalene acetic acid, and benzyladenine.…”