Effect of polymeric biocide polyhexamethylene guanidine hydrochloride on morpho-physiological and biochemical parameters of wheat seedlings under copper stress

Lyoshyna, L.; Tarasyuk, Oksana; Bulko, Olga; Rogalsky, Sergiy; Kamenieva, T.M.; Кучук, М. В.

doi:10.15407/agrisp7.01.049

Cited by 5 publications

(2 citation statements)

References 33 publications

(46 reference statements)

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“…Furthermore, Atabayeva et al [23] indicated that high doses of Cu treatment suppressed shoot length and dry weight in wheat varieties significantly. In addition, Lyoshyna et al [24] proved a notable reduction in green mass and shoot length of wheat seedlings after Cu application. To our knowledge, this is the first study to show that Trifloxystrobin pretreatment reduces Cu stress-induced growth restriction in wheat.…”

Section: Resultsmentioning

confidence: 99%

Trifloxystrobin Pretreatment Alleviates Excessive Copper Stress in Wheat (Triticum aestivum L.)

Macar

Karaköy

2023

Cumhuriyet Science Journal

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Protective role of Trifloxystrobin pretreatment against excessive copper-related abiotic stress in Triticum aestivum L. was determined in two Turkish wheat cultivars, Sönmez and Gerek 79. Ten-day-old seedlings were pretreated with 20 µM and 80 µM Trifloxystrobin. A group of seedlings was harvested without exposure to Trifloxystrobin as a control. Two days after, seedlings were treated with copper(II) chloride. Seedlings were harvested on the 20th day after sowing. The growth level of the groups was evaluated by measuring the length, fresh weight and shoot dry weight of shoots. Chlorophyll a + b, carotenoid and anthocyanin contents as well as proline levels were assessed. Lipid peroxidation and total activities of superoxide dismutase, peroxidase and glutathione reductase were analyzed to predict the oxidative stress levels. Both cultivars exhibited similar responses to the treatments. Trifloxystrobin doses did not cause damage on plants when applied alone. Seedlings subjected to excessive doses of copper showed significant reductions in growth parameters, as well as chlorophyll and carotenoid pigments. Conversely, copper caused a remarkable increase in anthocyanin, proline and malondialdehyde accumulation. Superoxide dismutase and peroxidase activities increased, while glutathione reductase activity decreased in copper-stressed plants. Trifloxystrobin pretreatment strengthens the antioxidant defense system. All parameters were positively affected by Trifloxystrobin pretreatment. As the dose of Trifloxystrobin increased, the severity of stress decreased in both genotypes. Trifloxystrobin pretreatment is a promising method for reducing copper-induced damage in T. aestivum.

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Section: Resultsmentioning

confidence: 99%

Trifloxystrobin Pretreatment Alleviates Excessive Copper Stress in Wheat (Triticum aestivum L.)

Macar

Karaköy

2023

Cumhuriyet Science Journal

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“…It should also be noted that our earlier studies indicated the possibility of using PHMGH not only in veterinary medicine and animal breeding but also in plant cultivation, for instance, to to stimulate seed germination and germination energy (Lysytsya A et al, 2013;Lyoshyna L et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Effect of hydrochloric polyhexamethylene guanidine (PHMGH) and polyhexamethylene biguanidine (PHMBH), also in combination with plant essential oils and ZnO nanoparticles on some eukaryotic cattle and pig cells

Lysytsya¹,

Kryvoshyya²,

Kvartenko³

et al. 2022

Agric. sci. pract.

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Aim. To investigate both toxic (hemolytic), and stimulating effects of two polymeric derivatives of guani- dine, in particular, polyhexamethylene guanidine (PHMG) and polyhexamethylene biguanidine (PHMB) both in the hydrochloride form, on eukaryotic cells depending on the concentration of the preparation; to study the possibility of using wound-healing and stimulating properties of these preparations in veterinary medicine. Methods. The hemolytic activity (toxicity) of PHMGH and PHMBH preparations in the concentration of 0.1% towards cattle and pig erythrocytes was determined by titration. Primary cell cultures of fetal kidney cells of calves and piglets were used to determine the influence of PHMGH and PHMBH both alone and in combination with the following biologically active substances: essential oils of Pinus sylvestris, Eucalyptus globulus, Citrus sinensis, Monarda didyma, ZnO nanoparticles (size c. 25 nm), and electrochemically acti- vated water – anolyte (Eh = –800 mV, pH 6.5–7.0). The concentration of the cells in the nutrient medium was determined via photocolorimetry. Results. It was found that depending on the concentration, PHMGH, and PHMBH preparations can cause the lysis of erythrocytes, and stimulate cell proliferative activity, including the formation of a monolayer of kidney cells of calves and piglets They cause hemolysis of cattle erythro- cytes in the concentrations commonly used for disinfection, i.e., about 0.1 %, in the average titers of 1 : 7 for PHMGH and 1 : 2.5 for PHMBH. Therefore, PHMBH shows greater hemolytic (biocidal) activity for cattle erythrocytes than PHMGH (in ≈2.8x). The high molecular weight fraction of PHMBH (M2 ≈ 2,000–7,000 Da) demonstrated a lower (in ≈2.4x) hemolytic activity than the low molecular weight basic fraction (M1 ≈ 500–2,000 Da). The experiments on the kidney cell cultures of pigs and cattle have shown that at non-toxic concentrations (10–5 %) PHMBH can effectively stimulate (from 27 to 65 % increase) the proliferative activ- ity of eukaryotic cells and accelerate the formation of a monolayer of cells. The combinations of PHMGH with some essential oils of medicinal plants also show a good effect (from 52 to 95 % increase), and PHMBH shows a good effect with oil of pine for pig kidney cells (20 % increase) and oil of horsemint for cattle kidney cells (67 % increase). Conclusions. PHMGH and PHMBH can possibly be used in agricultural production not only as disinfectants or antiseptics, but also in wound healing. Although their toxicity is also significant to eukaryotic cells, yet they can possibly be used in veterinary medicine in low concentrations (0.005–0.5 %) for the treatment of wounds of various origin, including burns, in the composition of ointments, gels, bandages, or plasters, which we have presently in investigation.

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Biotechnology of groundwater purification for water supply systems, using Gallionella and Lepthothrix ferrobacteria

Kvartenko,

Lysytsya,

Shadura

et al. 2024

Agric. sci. pract.

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Aim. The aim of the study was to consider the possibility of using the consortia of chemolithoautotrophic ferrobacteria from Gallionella genus and heterotrophic bacteria from Lepthothrix genus for the biological method of groundwater purification. Methods. The photocolorimetric method to determine the concentrations of ammonium and iron ions, the titrimetric method to determine the hydrocarbon and total alkalinity, the method of determining the permanganate oxidizability using the Kubel method, the potentiometric method to determine the values of рН and Еh, the electronic microscopy using the X-ray spectral analysis of matrix structures of bio-minerals, microbiological and statistical methods. Results. The main technological parameters of the water deironing process were defined as follows: the filtration velocity of the bioreactor – 7–11 m/h, and of the filters – 3.5–5 m/h; the filter-cycle duration – 48 h. It was found that the application of the two-stage technology of biological deironing in the bioreactor and filters provided for the possible removal of Fe2+ compounds up to 5.0 mg/cdm, ammonium nitrogen — up to 1.5 mg/cdm, soluble organic substances by PO – up to 6.0 mg O2/cdm. It was determined that the optimal parameters for the process of biological purification of neutral groundwaters, containing increased concentrations of Fe2+ cations were as follows: рН 7.0–7.2; hydrocarbon alkalinity 2.5–2.2 mmol/cdm; content of soluble oxygen – 1.5–2.0 mg/cdm. The ability of concentrated (Dos 200 mg/cdm) matrix structures of Gallionella and Lepthothrix ferrobacteria to remove Cr6+ ions from natural groundwaters was determined. The study found no considerable differences in the efficiency of applying disinfectants, produced using polyhexamethylene guanidine chloride (PHMGchl) or polyhexamethylene biguanidine chloride (PHMBchl). In concentrations of 0.25–0.5 %, they effectively disinfect pathogenic microorganisms, including Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. The same was found true for the bacteria, most common in the systems of technical reverse water supply, the systems of water circulation, plant watering, and fire tanks, including Aeromonas hydrophila, Aeromonas salmonicida, Pseudomonas sp., E. coli, Flavobacterium columnare, Micrococcus lysodeikticus. Conclusions. This was the first study on the possibility of applying the biotechnology of groundwater purification from excessive amounts of iron in conditions of uneven hydraulic burden, notable for the water supply systems of rural areas and most agricultural enterprises in the north-western and northern regions of Ukraine. The specificities and perspectives of PHMGchl application in the systems of water preparation were studied. It was found that from the standpoint of safety and efficiency, the use of the water deironing processes involving iron bacteria was reasonable in the systems of water consumption and technical water supply, and the disinfection of water using PHMG was possible only in the second case, due to some toxicity of the preparation. The optimal parameters for the process of biological purification of neutral groundwaters, containing increased concentrations of Fe2+ cations were determined.

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Effect of polymeric biocide polyhexamethylene guanidine hydrochloride on morpho-physiological and biochemical parameters of wheat seedlings under copper stress

Cited by 5 publications

References 33 publications

Trifloxystrobin Pretreatment Alleviates Excessive Copper Stress in Wheat (Triticum aestivum L.)

Trifloxystrobin Pretreatment Alleviates Excessive Copper Stress in Wheat (Triticum aestivum L.)

Effect of hydrochloric polyhexamethylene guanidine (PHMGH) and polyhexamethylene biguanidine (PHMBH), also in combination with plant essential oils and ZnO nanoparticles on some eukaryotic cattle and pig cells

Biotechnology of groundwater purification for water supply systems, using Gallionella and Lepthothrix ferrobacteria

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