Effect of polyploidization in the production of essential oils in Lippia integrifolia

Investigation of Morphological and Anatomical Changes in Catharanthus Roseus (L.) G. Don Due to Colchicine Induced Polyploidy

“…The increase in the size of Vinca pollen diameter (Table, 3 and Fig., 3) was documented in the present study which agrees with previous observations of Abdoli et al (2013) on Echinacea purpurea (L.) who found that the diameter of tetraploid pollen grains was considerably larger than those in the corresponding diploid plants, Kumar and Dwivedi (2014) applied aqueous solution of colchicine of 0.3, 0.4, 0.5, 0.6% to Brassica campestris L. seedlings and found that pollen diameter in autotetraploid and diploid plants was 67.95±0.02 and 26.05±0.28 μm, respectively and Iannicelli et al (2016) exposed Lippia integrifolia explants to 0.01% of colchicine and obtained differences in pollen grains size between the new tetraploids and the diploids. In addition, our results displayed the increment in tetraploid pollen viability percentage (Table, 3 and Fig., 4) over diploid, which consistent with Oates et al (2012) on in vitro treated shoot apices with 30 mM oryzalin for 5 days as increased pollen staining of allotetraploids of Rudbeckia subtomentosa compared to their diploid counterparts.…”

“…In the present study colchicine induction in Vinca produced tetraploid significantly different in leaf area and leaf thickness (Table, 2). These results were in harmony with those of Tharawoot et al (2012) on Impatiens patula Craib who found that tetraploid plants had the highest average of leaf thickness than diploid plants, Kumar and Dwivedi, (2014) found a reduction in leaf length and leaf breadth of Brassica campestris L. tetraploid and Iannicelli et al (2016) reported that tetraploid displayed considerable changes from the field mother plant in size of Lippia integrifolia leaves. Similarly, the increment in the mean length and width of the stomata of tetraploid plants than in diploid plants (Table, 2 and Fig., 2) hence, stomata size is another source of data which can be used as a tool for primarily verify the status of tetraploid.…”

Induction of Synthetic Polyploids and Assessment of Genomic Stability in Lippia alba

“…The metabolic activity may be increased due to alteration of gene expression or changes in the concentration of the secondary metabolites (Fasano et al, 2016;Iannicelli et al, 2020). Evidence of the genetic duplication affecting metabolic profiles of different plant species has been widely reported (Caruso et al, 2011;Dehghan et al, 2012;Trojak-Goluch and Skomra, 2013;Gomes et al, 2014;Xu et al, 2014;Tavan et al, 2015;Iannicelli et al, 2016) which reinforces the application of genome duplication protocols for manipulating the biosynthesis of compounds of economic interest.…”

“…The induction of polyploids has been widely used as a strategy to investigate the effects of artificial genomic duplication on several plant species. Morphological, histological, physiological, agronomic, and genomic traits have been evaluated in different studies (Adams and Wendel, 2005;Buggs et al, 2008;Rêgo et al, 2011;Hegarty et al, 2013;Gomes et al, 2014;Tavan et al, 2015;Gao et al, 2016;Iannicelli et al, 2016;Yan et al, 2016;Sadat-Noori et al, 2017;Salma et al, 2017;Zhou et al, 2017). Although polyploidy induction has been widely recognized as an important strategy for chromosome duplication in plants, the protocols are still associated with low efficiency.…”

Induced autopolyploidy—a promising approach for enhanced biosynthesis of plant secondary metabolites: an insight

“…(dissolved in 2% DMSO)➔ sterile H 2 O washing Shoot tip Colchicine; 0.3%; 12 h Diploid➔tetraploid (2n=4x=56) Increase in betulinic acid (69.73%), oleanolic acid (42.76%), ursolic acid (140.67%) [ 25 ] Stevia rebaudiana Bertoni Treatment in field condition NM Colchicine; 2.5%; NM Diploid➔tetraploid (2n=4x=44) Increase in rebaudioside-A (6.21%) [ 26 ] Colchicine; 1.50%; NM Mixoploid Increase in steviosisde (13.50%) Tetradenia riparia Submerged in colchicine soln. Seed Colchicine; 0.01 g/l; 24 h Diploid➔tetraploid (2n=4x=) Increase in essential oi production (3.5-fold) [ 27 ] Echinacea purpurea L. MS + 0.4 mg/l BA + 0.01 mg/l NAA + Colchicine Petiole Colchicine; 100 mg/l; 37 d Diploid➔tetraploid (2n=4x=44) Tetraploid➔octaploid (2n=8x=88) Increase in cichoric acid content in octoploid (13.76 and 29.84 mg/g) and tetraploid (10.38 and 22.32 mg/g) than diploid plantlets (8.21 and 18.42 mg/g) aboveground and underground parts, respectively [ 28 ] Miscanthus × giganteus NM NM Colchicine; NM; NM Triploid➔hexaploid (2n=6x=114) Lower phenolic compounds in hexaploids (1266 μg/g and 1349 μg/g) than triploid (1570 μg/g and 2268 μg/g) [ 29 ] Lippia integrifolia (MS + 2.2 μM BA) + colchicine Nodal segment Colchicine; 0.01%; 15 d Diploid➔tetraploid (2n=4x=72) Increase in total monoterpene (9%) [ 30 ] Pogostemon cablin MSL + colchicine➔ shaking at 100 rpm at 25°C Cluster bud Colchicine; 0.05%; 72 h Tetraploid➔octaploid (2n=8x=128) Increase in patchoulic alcohol (2.6-fold) [ 31 ] Chamaemelum nobile L. MSL + 1 μM NAA + 0.5 μM BAP + Colchicine; 25 °C; Dark for 12 h Callus Colchicin...…”