Effect of polysaccharides extracts of algae Ulva rigida on growth, antioxidant, immune response and resistance of shrimp, Litopenaeus vannamei against Photobacterium damselae

Abstract

This study was carried out to investigate the effect of water‐soluble polysaccharides extract of algae Ulva rigida (WPU) as dietary supplement on growth performance, antioxidant enzyme activity, lysozyme and phenoloxidase activity, and resistance of shrimp (Litopenaeus vannamei) subjected to bacterial infection with Photobacterium damselae. Three replicate groups of shrimp (1.0 g) were fed four diets containing four levels, 0 or control, 0.5, 1 and 1.5 g/kg of WPU for 8 weeks over the growth trial. Thereafter, 30 shrimps from each dietary treatment were infected with bacteria P. damselae to evaluate disease resistance of infected shrimp. The results of this study showed that WPU was effective as a growth promoter for L. vannamei. The best growth rate was observed in shrimp fed 1.5 g/kg of WPU diet. Regarding antioxidant defences, the diets supplemented with three levels of WPU stimulated glutathione peroxidase and catalase activates in experimental shrimps. MDA content of L. vannamei‐fed diet containing WPU 1.5 and WPU 1.0 was lower than WPU 0 and WPU 0. 5 diets. Also, lysozyme and phenoloxidase activities of shrimp receiving WPU at 1.0 and 1.5 level were significantly higher than those fed WPU 0 and WPU 0.5 diets. In addition, using WPU extract in all diets decreased mortality in L. vannamei in a dose‐dependent manner after challenge with P. damselae. These results suggest that incorporation of water‐soluble polysaccharides from green algae U. rigida at 1.5 g/kg doses improves growth and antioxidant activity and enhances the immune responses in shrimp L. vannamei.

The ameliorative role of ascorbic acid against blood disorder, immunosuppression, and oxidative damage of oxytetracycline in rainbow trout (Oncorhynchus mykiss)

“…This is well established that increased reactive oxygen species (ROS) levels will lead to oxidative stress, which may negatively affect the sh and crustaceans' health and cause structural damage in tissue cells (Akbary and Aminikhoei 2018;Tavabe et al 2020). Antioxidant defense system includes some pivotal enzymes such as superoxide dismutase (SOD) and catalase (CAT) that tend to prevent ROS formation (Yonar et al 2014).…”

Growth, survival, nutritional value and phytochemical, and antioxidant state of Litopenaeus vannamei shrimp fed with premix extract of brown Sargassum ilicifolium, Nizimuddinia zanardini, Cystoseira indica, and Padina australis macroalgae

“…According to the results of this study, the inclusion of MPE in the diet improved the antioxidant activity of the L. vannamei shrimp as compared with shrimps on the control diet. Akbary and Aminikhoei (2018a) reported that the inclusion of 1.5 g kg − 1 of U. rigida algae extract in the diet of shrimps signi cantly increased the activity of SOD and reduced glutathione.…”

The ameliorative role of ascorbic acid against blood disorder, immunosuppression and oxidative damage of oxytetracycline in rainbow trout (Oncorhynchus mykiss)

“…Similar to our results, Roosta et al (2014) observed an increase in the activity of mucus alkaline phosphatase in Rutilus rutilus caspicus following dietary supplementation with high levels of AA. This is well established that increased reactive oxygen species (ROS) levels will lead to oxidative stress, which may negatively affect the sh and crustaceans' health and cause structural damage in tissue cells (Akbary and Aminikhoei 2018;Tavabe et al 2020). Antioxidant defense system includes some pivotal enzymes such as superoxide dismutase (SOD) and catalase (CAT) that tend to prevent ROS formation (Yonar et al 2014).…”