Effect of Polyspecific Rabbit Anti–Immunoglobulin Antisera on Human Lymphocytes &lt;i&gt;in vitro&lt;/i&gt;

Ss, Frøland; Natvig, J. B.

doi:10.1159/000230341

Cited by 46 publications

(33 citation statements)

References 13 publications

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“…Injections were given with Freund's complete adjuvant. The specificity of the antisera obtained has been de scribed earlier [11]. Normal rabbit serum (NRS) from the same stock of rabbits was used for control cultures.…”

Section: Methodsmentioning

confidence: 99%

“…Venous blood (40-90 ml) was drawn into sterile bottles containing preservative-free heparin (10 IU/ml blood). Lymphocytes were then isolated by the Isopaque-Ficoll-centrifugation technique, as described earlier [4,11], The cells obtained were counted in a Burker haemocytometer and resuspended in culture medium (McCoy's 5a with 20% fetal calf serum or, later, Medium 199 with Earle's balanced salt solution, both obtained from Grand Island Biological Company, New York, N. Y.…”

Section: Methodsmentioning

confidence: 99%

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Effect of Polyspecific Rabbit Anti-Immunoglobulin Antisera on the Response of Human Lymphocytes in Mixed Lymphocyte Culture

Ss¹,

Natvig²

1971

Int Arch Allergy Immunol

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The effect of pretreatment of human peripheral lymphocytes with rabbit anti-F(ab′)₂ on reactivity in mixed lymphocyte cultures (MLC) was studied in 19 experiments using a one-way system and in 10 experiments with two-way stimulation. In some ‘two-way cultures’, an additive effect of allogeneic lymphocytes and undiluted anti-F(ab′)₂ was observed. In the one-way system and in the majority of the two-way MLC tests a marked potentiating effect of the two stimulants could be seen, the activity in most cases being much higher than the sum of the single effects. With neither system could inhibition be observed. In the one-way MLC system, the effect of diluting the antiserum was studied to see if any inhibitory effect could be discovered. At the highest dilution studied (1:10,000), however, there was still no inhibition. It is concluded that the present study does not give evidence of immunoglobulin receptors necessary for the MLC response. The possibility that the lymphocytes stimulated by anti-F(ab′)₂ may be different from the cells engaged in the MLC response, is discussed.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Effect of Polyspecific Rabbit Anti-Immunoglobulin Antisera on the Response of Human Lymphocytes in Mixed Lymphocyte Culture

Ss¹,

Natvig²

1971

Int Arch Allergy Immunol

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“…Lymphocytes were separated from venous heparin blood (10 U/ml of heparin) by the Isopaque-Ficoll gradient centrifugation technique [4,10]. Blood was ob tained from healthy adults, either donors at a blood bank or laboratory personnel.…”

Section: Sources Of Blood Lymphocytesmentioning

confidence: 99%

“…The cultured cells were washed thoroughly in HBSS before the rosette test was performed. The lymphocyte cul ture technique employed has been described in detail earlier [10,11].…”

Section: Lymphocyte Cultures With Mitogensmentioning

confidence: 99%

Human Lymphocytes with Receptors for IgG

Wisløff

Michaelsen³

1974

Int Arch Allergy Immunol

136

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Human lymphocytes with receptors for IgG (EA-RFC) were detected in peripheral blood by their ability to form rosettes with human erythrocytes sensitized with human (Ripley anti-CD) or rabbit IgG. EA-RFC were shown to be different from T-lymphocytes forming rosettes with sheep erythrocytes (E-RFC), both by direct studies of mixed rosette suspensions and by separation of EA-RFC and E-RFC by depletion of either EA-RFC or E-RFC by gradient centrifugation of the corresponding rosettes, as well as by fractionation of cells on HSA/anti-HSA columns, which very effectively and selectively retain EA-RFC. Proportions of EA-RFC were also within the normal range in patients with profound lack of T-cells and were not increased during in vitro lymphocyte culture in the presence of T-cell mitogens. The majority of EA-RFC was also distinct from B-lymphocytes as identified by membrane-bound Ig. Thus, EA-RFC could be separated from B-lymphocytes by fractionation on nylon columns retaining B-lymphocytes, but not EA-RFC, by fractionation on HSA/anti-HSA columns retaining EA-RFC but not B-lymphocytes, as well as by depletion of EA-RFC by gradient centrifugation of rosettes. The correlation between EA-RFC and B-lymphocytes in blood was also very poor, both in patients with pathologically low proportions of either normal or malignant B-lymphocytes as well as with abnormally high proportions. It is therefore concluded that EA-RFC represent a population of lymphocytes largely unrelated to human T- and B-lymphocytes as identified by conventional markers.

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“…Cultures without mitogen were always included. Lymphocyte transformation was determined by 'H-thymidine incorporation as reported earlier (10,15). The results are presented as disintegrations per minute (dpm) and as the calculated transformation index (TI): dpm in Cultures with Mitogen dpm in Cultures without Mitogen TI = Antibodydependent cytotoxicity.…”

Section: Methodsmentioning

confidence: 99%

Lymphocytes from synovial tissue of a boy with x‐linked hypogammaglobulinemia and chronic polyarthritis

Abrahamsen

Frøland

Kåss

et al. 1979

Arthritis & Rheumatism

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Lymphocytes were eluted from synovial tissue of a boy with X-linked hypogammaglobulinemia and chronic polyarthritis. The cell suspension contained 53% lymphocytes and 28% peroxidase-positive, macrophagelike cells. No B lymphocytes, 83% T lymphocytes, and 10% Fc-receptor-bearing lymphocytes were detected. Lymphocyte transformation was induced by polyclonal mitogens (phytohemagglutinin and pokeweed mitogen) whereas no response to antigens (purified protein derivative and Cadi& albicans antigen) was obtained. The eluted cells displayed antibodydependent cytotoxicity.

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Effect of Polyspecific Rabbit Anti–Immunoglobulin Antisera on Human Lymphocytes <i>in vitro</i>

Cited by 46 publications

References 13 publications

Effect of Polyspecific Rabbit Anti-Immunoglobulin Antisera on the Response of Human Lymphocytes in Mixed Lymphocyte Culture

Effect of Polyspecific Rabbit Anti-Immunoglobulin Antisera on the Response of Human Lymphocytes in Mixed Lymphocyte Culture

Human Lymphocytes with Receptors for IgG

Lymphocytes from synovial tissue of a boy with x‐linked hypogammaglobulinemia and chronic polyarthritis

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