Effect of Polysulfated Glycosaminoglycan on DNA Content and Proteoglycan Metabolism in Normal and Osteoarthritic Canine Articular Cartilage Explants

Sevalla, Katherine; Todhunter, Rory J.; Vernier‐Singer, Margaret; Budsberg, Steven C.

doi:10.1053/jvet.2000.9139

Cited by 23 publications

(13 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In contrast, Adequan ® alone did not increase the aggrecan core protein steadystate mRNA levels. This is similar to the findings of Sevalla et al 2000, in which a significant increase or decrease of aggrecan synthesis in articular cartilage explant culture was not shown, in response to PSGAG administration (28). However, the current data did not support the findings of Verbruggen et al (1999) who showed increased aggrecan synthesis and aggregate size, in response to PSGAGs in a three-dimensional model of chondrocyte culture (38).…”

Section: Discussionsupporting

confidence: 69%

“…ed chondroitin sulphate) to diminish matrix molecule degradation (28,34) and in some studies enhance matrix molecule synthesis (12). Little is known about the effects of PSGAGs upon gene transcription.…”

mentioning

confidence: 99%

“…Little is known about the effects of PSGAGs upon gene transcription. Recently, it was reported that PSGAG increased the DNA content of canine cartilage explants, presumably as a result of maintaining chondrocyte viability or stimulating mitotic activity (28). Nevertheless, the mechanism by which PSGAG affects articular matrix metabolism has remained unknown.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Polysulphated glycosaminoglycans modulate transcription of interleukin-1β treated chondrocytes in monolayer culture

Mertens¹,

MacLeod²,

Fubini³

et al. 2003

Vet Comp Orthop Traumatol

View full text Add to dashboard Cite

SummaryThe ability of polysulphated glycosaminoglycans (PSGAGs, Adequan®) to modulate the transcription of major articular cartilage matrix proteins and enzymes was examined. Northern blot analyses were used to compare steady-state mRNA levels of type-II procollagen, aggrecan core protein, matrix metalloproteinase (MMP)-1, MMP-3, tissue inhibitor of metalloproteinase (TIMP)-1, and TIMP-3 in equine chondrocytes grown in monolayer culture. The groups included: control, low-dose (0.1mg/ml) Adequan®, high-dose (1.0mg/ml) Adequan®, IL-1 β (10ng/ml), IL-1 β with low-dose Adequan®, and IL-1 β with high-dose Adequan®. The responses (p<0.05) to treatment were compared with one-way analysis of variance and post hoc least significant difference test. Interleukin-1 β significantly decreased steady-state levels of type-II procollagen (0.5x) and aggrecan core protein (to undetectable levels) and significantly increased MMP-1 (11.7x), MMP-3 (1.8x), TIMP-1 (1.9x), and TIMP-3 (5.1 x). Adequan® alone significantly increased steady-state mRNA levels of type-II procollagen (1.6x) and decreased MMP-1 (0.3x at high-dose only), MMP-3 (0.5x), and TIMP-1 (0.3x at high-dose only). In IL-1 β-treated chondrocytes, Adequan® significantly increased steady-state mRNA levels of type-II procollagen (1.4x of IL-1 β-treated levels), aggrecan core protein (IL-1 β -treated had no detectable expression), and MMP-3 (3.6 x at high dose) and significantly decreased steady-state mRNA levels of MMP-1 (0.3 x), and TIMP-1 (0.2x). This study is the first to demonstrate that Adequan® alters steady-state mRNA levels of key matrix proteins and enzymes; that it counteracts some of the deleterious effects of IL-1 β; and that these effects are in part dose-dependent.

show abstract

Section: Discussionsupporting

confidence: 69%

mentioning

confidence: 99%

See 1 more Smart Citation

Polysulphated glycosaminoglycans modulate transcription of interleukin-1β treated chondrocytes in monolayer culture

Mertens¹,

MacLeod²,

Fubini³

et al. 2003

Vet Comp Orthop Traumatol

View full text Add to dashboard Cite

show abstract

“…Durch diese Mechanismen können GAGPs als chondroprotektive Arzneimittel Knorpelschädigungen vorbeugen, hinauszögern oder sogar umkehren. (10,13,19,22,28,31).…”

Section: In Das Entzündungsgeschehen Eingreifenunclassified

Klinische Wirksamkeit von Glukosaminoglykanpolysulfat

Baisch¹

2009

pferde spiegel

View full text Add to dashboard Cite

“…PSGAG seems to have the potential for anti-inflammatory activity and direct stimulation of cartilage neosynthesis. Various studies have shown that PSGAG can stimulate hyaluronan, collagen, and GAG synthesis as well as chondrocyte replication; however, effects seem to be concentration and joint dependent, with some effects only being obtained with intra-articular injection [141,143].…”

Section: Polysulfated Glycosaminoglycanmentioning

confidence: 99%

Managing chronic arthritis

Malone

2002

Veterinary Clinics of North America: Equine Practice

View full text Add to dashboard Cite

Many compounds are being investigated for the control of symptoms of osteoarthritis in people and animals. Ideally, treatment should include analgesia, inflammation control, and chondroprotection. With further progress in this area, combination therapies tailored to the needs of the individual animal should enable us to maximize efficacy and minimize side effects. Only a few of the newer therapies and pharmaceutic agents have been investigated in the horse, however. With more rigorous investigation, they may be determined to be ineffective or unsafe. Meanwhile, as much information should be gathered from manufacturers as possible so as to ensure that appropriate recommendations are made.

show abstract

Effect of Polysulfated Glycosaminoglycan on DNA Content and Proteoglycan Metabolism in Normal and Osteoarthritic Canine Articular Cartilage Explants

Abstract: If the results of this study extend to in vivo use, treatment with PSGAG may modify the progression of OA in articular cartilage by maintaining chondrocyte viability or stimulating chondrocyte division as well as protecting against extracellular matrix degradation.

Cited by 23 publications

References 25 publications

Polysulphated glycosaminoglycans modulate transcription of interleukin-1β treated chondrocytes in monolayer culture

Polysulphated glycosaminoglycans modulate transcription of interleukin-1β treated chondrocytes in monolayer culture

Klinische Wirksamkeit von Glukosaminoglykanpolysulfat

Managing chronic arthritis

Contact Info

Product

Resources

About