Effect of polyunsaturated fatty acid supplementation on biophysical parameters and chilling sensitivity of ewe oocytes

Zeron, Yoel; Sklan, D.; Arav, Amir

doi:10.1002/mrd.1156

Cited by 177 publications

(159 citation statements)

References 26 publications

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“…This may be significant because PUFAs are known to influence follicle number during natural cycles in ruminants (Zeron et al 2002, Wathes et al 2007). …”

Section: Dietary Effects On Ovarian Follicular Steroidogenesismentioning

confidence: 99%

Dietary omega-3 and -6 polyunsaturated fatty acids affect the composition and development of sheep granulosa cells, oocytes and embryos

Wonnacott¹,

Kwong²,

Hughes³

et al. 2010

Reproduction

131

104

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The evidence that omega-3 (n-3) and -6 (n-6) polyunsaturated fatty acids (PUFAs) have differential effects on ovarian function, oocytes and embryo quality is inconsistent. We report on the effects of n-3 versus n-6 PUFA-enriched diets fed to 36 ewes over a 6-week period, prior to ovarian stimulation and follicular aspiration, on ovarian steroidogenic parameters and embryo quality. Follicle number and size were unaltered by diet, but follicular-fluid progesterone concentrations were greater in n-3 PUFA-fed ewes than in n-6 PUFA-fed ewes. The percentage of saturated FAs (mostly stearic acid) was greater in oocytes than in either granulosa cells or plasma, indicating selective uptake and/or de novo synthesis of saturated FAs at the expense of PUFAs by oocytes. High-density lipoproteins (HDLs) fractionated from sera of these ewes increased granulosa cell proliferation and steroidogenesis relative to the FA-free BSA control during culture, but there was no differential effect of n-3 and n-6 PUFAs on either oestradiol or progesterone production. HDL was ineffective in delivering FAs to embryos during culture, although n-6 PUFA HDL reduced embryo development. All blastocysts, irrespective of the treatment, contained high levels of unsaturated FAs, in particular linoleic acid. Transcripts for HDL and low-density lipoprotein (LDL) receptors (SCARB1 and LDLR) and stearoyl-CoA desaturase (SCD) are reported in sheep embryos. HDL reduced the expression of transcripts for LDLR and SCD relative to the BSA control. The data support a differential effect of n-3 and n-6 PUFAs on ovarian steroidogenesis and pre-implantation development, the latter in the absence of a net uptake of FAs.

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“…This may be significant because PUFAs are known to influence follicle number during natural cycles in ruminants (Zeron et al 2002, Wathes et al 2007). …”

Section: Dietary Effects On Ovarian Follicular Steroidogenesismentioning

confidence: 99%

Dietary omega-3 and -6 polyunsaturated fatty acids affect the composition and development of sheep granulosa cells, oocytes and embryos

Wonnacott¹,

Kwong²,

Hughes³

et al. 2010

Reproduction

131

104

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“…Yet, given the beneficial effect on oocyte maturation and early embryo development of α-linolenic acid, which is a precursor of EPA and DHA, it is reasonable to anticipate that, provided there is adequate antioxidative protection, there may be specific beneficial effects of dietary supplements of fish oil on the oocyte and early embryo. The study by Zeron et al (2002) demonstrating greater numbers of high quality oocytes with improved membrane integrity on chilling when ewes received diets supplemented with fish oil adds credence to this anticipation of specific benefits. So too does the recent observation by Warzych et al (2011) of enhanced expression of a marker gene (EEF1A1) for oocyte competence in the oocytes of pigs given a dietary supplement of docosahexaenoic acid.…”

Section: Introductionmentioning

confidence: 85%

Impact of contrasting fish oil concentrations in the diet on ovine embryo development <i>in vivo</i> and of corresponding diet-specific derivative sera during <i>in vitro</i> culture

McEvoy¹,

Önal²,

Speake³

et al. 2012

J. Anim. Feed Sci.

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In the first of two experiments, numbers of ovulations and day 5 embryo yields were unaffected by fish oil (0, 3 or 6% w/w) in ewe diets; oestradiol concentrations and numbers of large ovarian follicles were increased. Fish oil tended to suppress embryo development and limit blastocyst diameter and cell number but not protein synthesis. In the second experiment IVF-derived embryos were cultured in medium supplemented with foetal calf serum (FCS) or, as in vitro equivalents to Experiment 1, serum from ewes fed rations supplemented with 0 (C), 3 (3F) or 6 (6F)% fish oil. Cleavage rates were not affected by serum source. More Grade 1 to 1.5 blastocysts were produced when using 3F serum (32% of blastocysts) than with C (27%; ns) or 6F (16%; P<0.05) or FCS (10%; P<0.005). It is suggested that at less than 3% inclusion in the diet of ewes, there may be beneficial effects of fish oil fatty acids on embryo production in vivo and of the corresponding ewe serum during in vitro culture. Higher concentrations are counter-productive.

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“…Not only intracellular lipids, but also the lipid composition of the membranes contributes to differences in sensitivity to cooling injuries. Some factors, such as the origin of the oocyte or the embryo (in vitro produced or in vivo derived, species, breed, physiological state and nutrition of donor), proved to be determinant for the lipid content (ABE et al, 2002;GENICOT et al, 2005;McEVOY et al, 2000;STEEL;HASLER, 2004;ZERON et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Informação estrutural de lipídios a partir de um único embrião equino pela técnica de MALDI-MS

Gonçalves

Ferreira

Orlandi

et al. 2014

Braz. J. Vet. Res. Anim. Sci.

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It was reported the potential of MALDI-MS for the characterization of lipid species present in a single equine embryo, and studied some lipid structures detected by collision induced dissociation (CID) experiments. In the positive ion mode spectrum, it were observed mostly protonated and sodiated species of sphingomyelins (SM), phosphatidylcholines (PC) and triacylglycerols (TAG). In the negative ion mode, it were observed phosphatidylethanolamines (PE) and phosphatidylinositols (PI). MS/MS spectrum of most intense lipid ions was performed to show MALDI-MS/MS structural information potential.

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Effect of polyunsaturated fatty acid supplementation on biophysical parameters and chilling sensitivity of ewe oocytes

Cited by 177 publications

References 26 publications

Dietary omega-3 and -6 polyunsaturated fatty acids affect the composition and development of sheep granulosa cells, oocytes and embryos

Dietary omega-3 and -6 polyunsaturated fatty acids affect the composition and development of sheep granulosa cells, oocytes and embryos

Impact of contrasting fish oil concentrations in the diet on ovine embryo development <i>in vivo</i> and of corresponding diet-specific derivative sera during <i>in vitro</i> culture

Informação estrutural de lipídios a partir de um único embrião equino pela técnica de MALDI-MS

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